4. RBC-Indices full name
MCV= • Mean corpuscular volume • Femto liter (fl)
MCH= • Mean cell hemoglobin • Pico gram (pg)
MCHC= • Mean corpuscular hemoglobin • % or gram (g)
RDW= • Red cell distribution width (RDW) • %
Useful for anemia
classification according to the size or content of RBC
give a available information especially in the nutrients anemia,
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Diagnostic Hematology - Dr.Alagbare
6. 6
Rule of 3
1-RBC x 9 → = PCV or Hct
2-RBC x 3 → = Hb
3-Hb x 3 → =PCV or Hct
4-PCV ÷ 3 →= 𝐻𝑏
5-PCV ÷ 9 →= 𝑅𝐵𝐶
The instrument calculate the Hct or PCV as
follow
Hct=MCV ×RBC count
The Hct or PCV calculated as follow
RBC x 9
Hb X 3
MCV x RBC
Diagnostic Hematology - Dr.Alagbare
7. what cause the Low RBC count and
low Hct with normal Hb?
cause falsely high calculations of MCH
and MCHC, respectively.
Ex .
• Hb 12g/dl
• Hct:29
• RBC: 3.2
MCHC= 12/27 x100 = 44
what cause the elevation of Hb on the
MCH and MCHC?
cause falsely high calculations of MCH
and MCHC, respectively.
Ex .
• Hb 18g/dl
• Hct:29
• RBC: 3.0
MCH= 18/3.0 x10 = 60
MCHC= 18/29 x 100 =62
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Diagnostic Hematology - Dr.Alagbare
8. Introduction of the new
parameters
• New of different parameters
available on complete blood
count (CBC) analyzers.
• Its has been approved and they
are put in routine practice
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Diagnostic Hematology - Dr.Alagbare
9. 9
New parameters Abbrev. Normal value
Nucleated RBC NRBC, zero
Immature granulocyte fractions IG% zero
Hemopoietic progenitor cell HPC % zero
Immature reticulocyte fraction IRF (%) 1.1-15.9
Immature platelet fraction IPF (%) 1.1-6.1
Reticulocyte hemoglobin (Hb)- Ret He (pg) 28.6-33.3
Diagnostic Hematology - Dr.Alagbare