3. -What is ADVIA2120i?
-Components of ADVIA2120i
-Principle
-Reagents and Loading reagents
-Running samples
-Quality Control
-Maintenance
4. The ADVIA2120/2120i Hematology System is a fully
automated diagnostic instrument with a throughput
of 120 samples / hour(CBC/diff)
The analyzer uses whole blood sample to provide
the following types of result :
-Complete blood count(CBC)
-CBC plus White cell differential count
-Reticulocyte absolute, percent & indices
count(retic)
-CBC/diff plus retic(CBC/diff/retic)
-CBC/retic
9. Data Management
Database 10,000-patient storage capacity
including graphics
Workstation Specifications
Computer Windows 2000,® 40-gigabyte
hard disk drive,256-MB memory, CD-RW,
full-size keyboard
10. The ADVIA2021i Hematology System is made up of 2
major components :
A- The analyzer contains all the electronic, pneumatic,
hydraulic & sampler mechanisms, as well as the on
board reagent storage for all reagents: Defoamer,
Wash solution except Sheath RINSE.
The waste system is a sub component of analyzer.
13. The auto sampler automatically transports,
mixes, identifies & aspirates samples in closed
tubes.
The auto sampler is a rack-style sampler with a
capacity of 150 sample tubes: 15 racks of 10
tubes. The individual racks are barcoded for
rack and position numbers.
14. In open tube
aspiration, sample
probe is immersed in
to the specimen.
Pressing the push to
aspirate plate , starts
the sampling
15. The capped vial is
inserted upside down
in to the centering
collar
16. This block is made up
of 8 plates which are
pathway for fluid, air
flow, valves & reaction
chambers.
Reaction Chambers are:
-Hb reaction chamber
-Baso reaction chamber
-RBC reaction chamber
-Retic reaction
chamber
-Perox reaction
chamber
17. Its made up of 2 ceramic discs
The rear disk is stationary .
The front disc rotates to shear or
divide sample into appropriate
aliquots for analysis
18. The perox optical assembly
directs light from halogen
lamp to the perox flow cell.
The use of a sheath stream
in the flow cell allows a
cell-by-cell measurement
of light scatter and
absorption.
19. The laser optical assembly uses
a laser light source.
This optical assembly is shared by
RBC/Plt, retic, and baso/lobularity
channels.
The use of a sheath stream in
the flow cell allows cell-by-cell
measurement of low- and
high-angle light scattering and
absorption.
20. The hemoglobin colorimeter assembly
takes voltage readings that correspond
to the amount of transmitted light that
passes through the reaction chamber.
The system uses the readings to derive
the hemoglobin concentration.
21. The perox sample & sheath
pumps are located on the
left side of the analyzer.
The RBC/baso/retic sample
& sheath pumps are
located on the right side
of the analyzer.
22. The touchpad, located on the lower right of the analyzer, by which you operate the
analyzer.
On – Provides power to the analyzer assemblies and power supplies. After On is pressed,
samples through the manual open- and closed-tube samplers can be processed within 2.5
minutes, while samples through the auto sampler can begin
processing within four minutes.
Standby – Sets the analyzer to a lower power state. To exit, press Standby.
Start/Stop Sampler – Starts the auto sampler operation or stops the auto sampler.
Eject Rack – Moves all racks within the auto sampler into the output queue.
Rack in Sampler – Lights up when a sample rack is in the auto sampler,
including the input queue.
Off – Turns off the
23. The manual barcode reader is used to enter
information from labels on sample tubes,
reagent containers, controls, and calibrators.
24. All analyzer waste is stored in
an 11-liter waste container.
This container can be a stand-alone
container, which has to be emptied
manually, or part of the automatic
waste removal system. When the fluid
level in either of the containers reaches
the maximum-level line (app. 8 liters),
an error message appears on the Status
line on the personal computer monitor.
The analyzer will not aspirate any more
samples until the waste container is emptied.
25.
26. Laminar flow is induced by injecting a slow
moving sample dilution into a stream of fast-
moving sheath fluid which prevents mixing.
The sheath fluid surrounds the diluted
sample and narrows it, so that cells flow
through the sensing zone in single file…this
process is known as hydrodynamic focusing.
31. To run patient samples from the manual open
tube sampler:
1.Run samples in the following order :
a. Controls(control label) optional
b. Patient samples(sample ID label)
2.Scan the tube label or enter the sample
information in the manual sample ID tab
32. NOTE :
Ensure the sample ID displays on the status
line before aspirating a sample
4.Aspirate the sample
a. Position tube so that probe is immersed
into mixed well sample
b. Press the aspirate plate sample light
flashes during aspiration ,when it stops
flashing , remove tube
5.Validate results when available
33. Recommended controls are ADVIA TESTpoint
Haematology Controls (Low, Normal and High) that
should be keep and store at 2-5 degree C.
*Run controls at the start of the patient workload,
evaluate all controls before reporting patient results.
Allow to reach the controls room temperature before
use.
*Mix controls properly before run.
*Scan tube barcode by barcode reader
*Sampler probe is immersed in to mix well sample
only deep enough (Approximately 0.25 inch)
*Press the aspirate plate.
34. Daily Maintenance
*System wash
Time 6.5 min
Analyser mode should be ready to run
At utilities menu, select Hydrolytic function
Select system wash, select 1 cycle and press
Start
NOTE: Automatic wash cycle has to be at
least 3 cycles a day.
35. Clean the shear Valve and aspiration pathway
in the UFC(Unified Fluid Circuit) by using 3%
bleach and Distilled water (DW)
36. Replace both sheath filter
1. PEROX filter (0.45 Um)
2. Laser side Filter (0.22 Um)
Clean air filter
Clean 50Um plunger
