Viscosity is a key parameter in the characterization of protein formulations. In this webinar we demonstrate how VROC® technology can be used to study applications such as protein structure (i.e. denaturation and folding) at a fraction of the sample volume required by conventional technologies.

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d
V1
d
Shear stress, t
𝛾 =
𝑉1
𝑑
𝜂 =
𝜏
𝛾
F
F
— What is Viscosity?

6. — Viscosity Fingerprint
Concentration Effect
pH Effect
Buffer Type
Excipient Type
Temperature
Denaturants
Others
Viscosity screening:
Injectability
Molecular Size in Formulation
Stability in Formulation

7. —Drug Development
7
Small Sample Volume
High-Throughput
Structure
High-Shear
Precision

8. — Viscometer/Rheometer-on-a-Chip

9. — Viscometer/Rheometer-on-a-Chip
𝜏 ~ Δ𝑃
𝛾 ~ 𝑄
𝜂 =
𝜏
𝛾

10. — Viscometers

11. — New Product Release!

12. — Smallest sample Volume
μ

13. — Dynamic Range
μ

14. — Dynamic Range
μ
0.01 1 100 10000 1000000
Shear Rate [1/s]

16. 


— Injectability

17. frictionpiston
R
L
RF w
n
n
b   )
2
(
2
Gauge Dn Db Ln Injection Rate, Q n Shear Rate Viscosity Force Difference %
mm mm mm ml/s 1/s N
29 0.184 6.35 12.7 0.1 1 163,511 30.00 42 0
29 0.184 6.35 12.7 0.1 0.8 173,730 14.03 21 50
29 0.184 6.35 12.7 0.1 0.5 204,389 9.53 17 60
𝜂 = 𝜂∞ +
(𝜂0 − 𝜂∞)
1 + (𝜏 𝛾)1−𝑛
— Non-Newtonian Therapeutics

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What is Intrinsic Viscosity?
How does viscosity
relate to molecular
interaction? 𝜂 = lim
𝐶→0
𝜂 𝑠𝑝
𝐶
𝜂 𝑠𝑝 = 𝜂 𝑟 − 1 and
𝜂 𝑟 = 𝜂/𝜂 𝑠
η
C

19. 𝜂 = 𝐾𝑀 𝑤
𝑎
𝑟ℎ =
3 𝑀 𝑤[𝜂]
10 𝜋 𝑁𝐴
1/3
𝑟ℎ
Complementary to DLS
*Brandrup 2001. Polymer Handbook
— Intrinsic Viscosity

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•
•
•
𝑟ℎ = 1.3 𝑛𝑚
*Lee et al. Analytical Chemistry 2005
— Molecular Weight and Size

21. —Concentration
μ

22. — Denaturation
𝛾 = 1480 1/𝑠

23. — Stability & Excipients
𝛾 = 1480 1/𝑠

24. — Denaturants
K. Kawahara and C. Tanford. J. Biological Chemistry, 1966

25. — Protein Therapeutics

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