Lab Report Name: ____________________
Section: ___________________
Microbes Everywhere
Exercise 1 - Finding Microbes:
Observations from Procedures:
2. Select six sources from your prioritized list that are from different areas of your home. Set up a data table similar to the one shown below to number your microbes and record information and observations about them for Exercise 1 and Exercise 2.
Location Of Microbes
Colony Growth Notes....
#
Description
Temp
Shape
Color
Size
Type
Observations
1
2
3
4
5
6
Use the hand magnifying lens to observe the growths of colonies within the tubes. Note their shape, size, color, and anything else distinguishing. Bacteria will grow in small circular colonies, whereas molds will spread out more and may look fuzzy. Yeasts tend to grow initially in tight, compact colonies and their color is somewhat darker than that of bacteria. Record what you see in the data table. Draw what you see for each. Try to deduce the types of microbes each cultured colony contains: bacteria, mold, or yeast. Some cultures may contain more than one type of microbe.
If some of the cultures are not yet well developed let all of the cultures continue to incubate for up to three additional days until you see visible growth in them all. Record final observations for each tube.
Questions:
From which samples did you observe the most and least microbial growth?
Why do you think there were differences in the number and types of microbes at the different sampling sites?
Did microbes from warmer or cooler sites multiply faster? Hypothesize why.
How could the information gained in this experiment be useful in your home?
Exercise 2 - A Closer Looks
Observations from Procedures:
Location Of Microbes
Gram Stain Notes.........
#
Description
Temp
Shape
Color
Size
Type
Observations
1
2
3
4
5
6
Examine the slides under your microscope. Describe and draw what you see.
Questions:
Was the structure or arrangement of the colonies of microorganisms different among what you identified as bacteria versus yeast versus mold?
Four slides are, of course, a very small sample, but regardless of this limitation, what can you hypothesize about differences in the microbe growth patterns? Does your hypothesis match the descriptions at the beginning of the experiment?
Were you able to see any individual microorganisms? If so what would you guess they are (e.g., mold, yeast, etc.)?
Do any of the slides appear to have more than one type of microorganism? Did you determine this by physical appearance of the culture or by color of stain?
Photosynthesis & Respiration
Exercise 1 – Photosynthesis Part 1
Bag mass ____ g + 1.5 g NaHCO3 = ____ Final mass (g)
Data Table 1. Photosynthetic rates.
Rate
Distance (mm)
Solution
starting measurement
Net photosynthesis
Respiration
Gross photosynthesis (net photosynthesis + res ...
1. Lab Report Name: ____________________
Section: ___________________
Microbes Everywhere
Exercise 1 - Finding Microbes:
Observations from Procedures:
2. Select six sources from your prioritized list that are from
different areas of your home. Set up a data table similar to the
one shown below to number your microbes and record
information and observations about them for Exercise 1 and
Exercise 2.
Location Of Microbes
Colony Growth Notes....
#
Description
Temp
Shape
Color
Size
Type
Observations
1
3. Use the hand magnifying lens to observe the growths of
colonies within the tubes. Note their shape, size, color, and
anything else distinguishing. Bacteria will grow in small
circular colonies, whereas molds will spread out more and may
look fuzzy. Yeasts tend to grow initially in tight, compact
colonies and their color is somewhat darker than that of
bacteria. Record what you see in the data table. Draw what you
see for each. Try to deduce the types of microbes each cultured
colony contains: bacteria, mold, or yeast. Some cultures may
contain more than one type of microbe.
If some of the cultures are not yet well developed let all of the
cultures continue to incubate for up to three additional days
until you see visible growth in them all. Record final
observations for each tube.
Questions:
From which samples did you observe the most and least
microbial growth?
Why do you think there were differences in the number and
types of microbes at the different sampling sites?
Did microbes from warmer or cooler sites multiply faster?
4. Hypothesize why.
How could the information gained in this experiment be useful
in your home?
Exercise 2 - A Closer Looks
Observations from Procedures:
Location Of Microbes
Gram Stain Notes.........
#
Description
Temp
Shape
Color
Size
Type
Observations
1
2
6. Questions:
Was the structure or arrangement of the colonies of
microorganisms different among what you identified as bacteria
versus yeast versus mold?
Four slides are, of course, a very small sample, but regardless
of this limitation, what can you hypothesize about differences in
the microbe growth patterns? Does your hypothesis match the
descriptions at the beginning of the experiment?
Were you able to see any individual microorganisms? If so
what would you guess they are (e.g., mold, yeast, etc.)?
Do any of the slides appear to have more than one type of
microorganism? Did you determine this by physical appearance
of the culture or by color of stain?
Photosynthesis & Respiration
Exercise 1 – Photosynthesis Part 1
Bag mass ____ g + 1.5 g NaHCO3 = ____ Final mass (g)
Data Table 1. Photosynthetic rates.
Rate
Distance (mm)
Solution
starting measurement
7. Net photosynthesis
Respiration
Gross photosynthesis (net photosynthesis + respiration)
Rate of photosynthesis (gross photosynthesis / 10 min)
Questions:
A. What molecules did the plant produce when exposed to light?
B. What resources were consumed to produce the molecule?
C. What part did light play in the reaction?
D. What molecule was consumed by the plant while it was in
the dark?
Plants consume Oxygen molecule (O2) . But oxygen can only be
consumed in conjunction with fuels to oxidize: glucose, amino
acidsand fatty acids at night in the process of respiration.
E. What molecule was produced by the plant while it was in the
8. dark?
In the dark, at night, plants respire just as we do, taking oxygen
molecules and glucose to produce ATP and carbon dioxide - the
later released as a gas
F. Where did the molecules go that were produced in the dark?
G. What critical service do plants provide humans?
H. Would animal life on Earth be possible without plants?
I. Would any life on Earth be possible without sunlight?
Exercise 2 – Photosynthesis Part 2
9. Data Table 2. Respiration results.
Experimental
Conditions
Starting distance (mm)
Final distance (mm)
Change in movement
(mm)
Time (min)
With Elodea
Without Elodea
Questions:
A. When you blew through the straw into the Bromothymol blue
solution, what gas did you add? What color change did you
observe in the solution and why?
10. B. What color change occurred after you placed the plant into
the respirometer and turned on the light?
C. What was happening when the solution in the horizontal
tubing started to move?
D. How long did it take the color change to occur after the
solution started to move?
E. Why did the color change?
F. When you repeated the experiment without a plant in the
respirometer, what happened and why?