1. Chapter 20: Instrumentation
Major Components
Depends on Type of Experiment
Absorption
Fluorescence
Emission
Source
Wavelength Selector
Sample
Detector
Signal Processor
Most Common
Placement depends on experiment
10. Instrument Components
Bandwidth – wavelength range passed by selector
High Quality: ~ 0.1 nm (monochromator)
Filter 50 nm
From Skoog, West, Holler
11. Sample Container
Holds the sample
Must be transparent in the region of interest
UV: Quartz
Vis: Glass
IR: Salt Crystals
12. 1. In the equation, A = εbc, what quantity is represented by "ε" ?
a) Absorptivity
b) Molar absorptivity
c) Path length
d) Concentration
2. Can one measure a smaller amount of a compound if it has
a high or low absorptivity
a) higher
b) lower
23. An 8.64 ppm solution of [FeSCN]Cl (149 g/mol) has a T of 0.295
at 580 nm when measured in a 1.00-cm cell. Calculate
the molar absorptivity coefficient for the complex
at this wavelength.
24. Cytochrome c has a molar absorptivity of 106,000 M-1
cm-1
.
A solution is prepared by diluting a 10.0 uM solution by a
hundred. What would be the absorbance of the
diluted solution if measured in a 1.00 cm cell.