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No-SelfisbetterthanSelf
Thesurprisingmechanismexplainingbiodiversity
StefanoMazzoleni
UniversityofNaplesFedericoII,Italy
www.ecoap.unina.it
VianadoCastelo8CongressoFlorestalNacional
12october2017
Thesurprisingmechanismexplainingbiodiversity:
frommodelsandtheoreticalreasoningto
experimentalevidence
fromexperimentalevidenceto
modelsandtheoreticalreasoning
observationofrealworldlogicalthinking
chickenandeggproblem
EmpiricalapproachTheoreticalapproach
InductionDeduction
Problem
Whattodonext?
Ineedmoredata!
Ineedamodel!
Idonotbelieve
inmodels!
SystemsBiology
studiesatdifferentinteractingomicscales
Keywords:genome,trascriptome,proteome,metabolome
clustersnetworks
SystemDynamics
complexsystemsassimpleprocessesinteractingintime
Keywords:stocksandflows,differentialequations,feedbacks
N°treespecies/ha
(DBH>10cm)
Boreal
forest
Temperate
forest
Tropical
forest
0
50
100
150
200
250
300
cMangroves
Openquestionsonbiodiversity
Whyplantsformrings?
Soilnegativefeedbackand
seedlingsurvivaldistribution
Distancefromparenttree
Numberofseeds(S)
NFandJanzen-Connelldistribution
(Packer&Clay2000,Nature)
Janzen-Connelldistribution
(Janzen1970,Am.Nat.Connel1971)
S
PR
Soilnegativefeedbackand
seedlingsurvivaldistribution
Probabilityofestablishment(P)
Numberofseeds(S)
Distancefromparenttree
P
NFandJanzen-Connelldistribution
(Packer&Clay2000,Nature)
Janzen-Connelldistribution
(Janzen1970,Am.Nat.Connel1971)
S
PR
Negativeplant-soilfeedback
andtropicalforestdiversity
Manganetal.2010,Nature
JCdistributionnotfoundinmangroves.Why?
NF,firstlyreportedforAmmophilaarenaria,speedsupprimary
successiononsanddunes(vanderPuttenetal.1993,Nature)
AmmophilaarenariaNFrelatedtosoilbornepathogensand
nematodes(vanderGoesetal.1995,NewPhytol.)
Plant-soilnegativefeedbackinecology
Allelopathyofplantlitter
15
?
Bonanomietal.2005.PlantEcology
Scirpusholoschoenus
Structureof
Scirpusholoschoenus
Seeds/m2 Seedlingnumber/150gsoil
Distancefromtussockcentre(m)
n°plantule/150gsuolon°semi/m2
Janzen-Connell
distributionofseedlings
OUT
IN
Nodifferencesinsoilanalysis
OUTINt-Test
Sabbia(%)
Argilla(%)
3730.5P=0.054
24.533.5P=0.12
38.536P=0.32
0.20.22P=0.27
7.417.46P=0.35
2.492.35P=0.11
0.280.28P=0.50
479496P=0.37
145139P=0.39
EC(mS/cm)
pH
SO(%)
P2O5(mg/kg)9.239.17P=0.48
28112716P=0.35
Parametrit-
Limo(%)
3730.5P=0.054
24.533.5P=0.12
38.536P=0.32
0.20.22P=0.27
7.417.46P=0.35
2.492.35P=0.11
0.280.28P=0.50
P=0.37
145139P=0.39
9.239.17P=0.48
P=0.35
K20(mg/kg)
CaO(mg/kg)
MgO(mg/kg)
Scirpusholoschoenus
Scirpusholoschoenus
Rootfunctionality
mgLi/kg
Distancefromtussockcentre(cm)
n°stems/25cm2
Menthaaquatica
Pulicariadesenterica
Islandsoffertility:facilitationonseedlingrecruitment
ProsopisglandulosaMedicagomarina
Totalnumberofreportednegativefeedbackcases-terrestrialecosystems:135
TotalnumberofJantzenandConneldistribution-terrestrialecosystems:197
-aquaticecosystems:0
-aquaticecosystems:1
TOTALSTUDY
CASES(N°)
INTER-SPECIFIC
FACILITATION(%)
INTRA-SPECIFIC
FACILITATION(%)
TERRESTRIAL
ECOSYSTEMS
155494.75.3
AQUATIC
ECOSYSTEMS
13265.134.9
Whyfacilitationismostlyheterospecific?
NegativefeedbackANegativefeedbackB
TheoreticalreasoningonNF
Bonamomi,GianninoandMazzoleni2005.Oikos
NF=0%
0
20
40
60
80
100
Biomass%
Time
GrA=0.6
GrB=0.5
TheoreticalmodellingofNF
7days
Control
Leafextracts
40days
Mazzolenietal.(2007)CommunityEcology
Modellingtheeffectsoflitterdecompositionontreediversitypatterns
Mazzolenietal.(2010)EcologicalModelling
Relativeabundance(%) Relativeabundance(%)
Modellingtheeffectsoflitterdecompositionontreediversitypatterns
Mazzolenietal.(2010)EcologicalModelling
Nutrientcycling
PROBLEM
Grasses-Sedges
Forbs
N-Fixing
Woody
SpeciesVegetationtypePlantmaterialDecomposition
(5)JunkuseffususL.Leaf-RootAerobic/Anaerobic
(2)CarexdistachyaDesf.Leaf-RootAerobic
(7)Phragmitesaustralis(Cav.)Trin.Leaf-RootAerobic/Anaerobic
(4)FestucadrymeiaM.etK.LeafAerobic/Anaerobic/Litterbag
(8)CoronillaemerusL.LeafAerobic/Anaerobic/Litterbag
(13)CistusmonspeliensisL.LeafAerobic/Anaerobic
(12)CistusincanusL.LeafAerobic
(16)QuercusilexL.LeafAerobic/Anaerobic/Litterbag
(25)TyphalatifoliaL.Leaf-RootAerobic/Anaerobic
(19)HederahelixL.LeafAerobic/Anaerobic/Litterbag
(6)Phleumsubulatum(Savi)AschetGr.LeafAerobic
(1)Ammophilalittoralis(Beauv.)Rothm.LeafAerobic
(3)DactylishispanicaRothLeaf-RootAerobic
(9)MedicagomarinaL.LeafAerobic
(10)MedicagominimaL.Leaf-RootAerobic
(11)MelilotusneapolitanaTen.Leaf-RootAerobic
(17)RosmarinusofficinalisL.LeafAerobic
(15)PinuspineaL.LeafAerobic
(14)FraxinusornusL.LeafAerobic
(23)TeucriumchamaedrysL.Leaf-RootAerobic
(24)TeucriumpoliumL.Leaf-RootAerobic
(21)PetrorhagiasaxifragaL.Leaf-RootAerobic
(22)PetrorhagiavelutinaGussLeaf-RootAerobic
(20)LobulariamaritimaL.Leaf-RootAerobic
(18)BellisperennisL.Leaf-RootAerobic
Functionalgroup
Wetland
Grassland
Wetland
Woodland
Grassland
Mobilesanddune
Grassland
Woodland
Mobilesanddune
Grassland
Grassland
Shrubland
Shrubland
Woodland
Shrubland
Woodland
Woodland
Wetland
Woodland
Grassland
Grassland
Grassland
Grassland
Grassland
Grassland
Bonanomietal.(2005)
Autotoxicity?
AC+
AC-
ControlQuercusilex
0Decomposition
days
1200120
HeterospecificConspecificLITTER
Toxicityexperiment
Littermaterial
1HNMR
EXPERIMENTALEVIDENCES
autotoxitywasdemonstrated,but:
thechemicalcompoundhadtobe:
DNA!
4-Species-specific
1-Resistant
2-Notremovablebycharcoal
3-Watersoluble
-unexpectedresultsoftimingofappearance:(
-timingcompatiblewithbuild-upofsoilsicknessinagriculture:)
Nutrientcycling
Modelidea
Inhibitoryeffectby
extracellularSELF-DNA
DNAconcentration
FreshlitterDecomposedlitterAgarosegel
501001502000ppm
Relativeabundance
Hederahelixundecomposed
Hederahelixdecomposed
for180days
13CCPMASNMR
13CCPMASNMR
DNA!
CONTROL+SELFDNA
Discovery(1)
>30plantspeciesNewPhytologist2015a
+HeterologousDNA+SelfDNA
DNAreleasebylitter
decompositionand
rootturnover
extracellularDNA
degradation
interactionswithminerals
andorganicmatter
uptake
Enhance
pathogen
attack
inhibitroot
functionality
inhibitcell
functionality
InhibitoryeffectofextracellularSELF-DNAasmechanismofNF
Inhibitoryeffectnotonlyforplants:
itisageneralbiologicalphenomenon!
Discovery(2)
NewPhytologist2015b
Historyofagriculture
No-SelfisbetterthanSelf!
No-SelfisbetterthanSelf!
BiodiversitySoilsickness
Butwhyplantsformrings?
?
???
Directionoftussockspread
Livingtillers
A)
Living
fineroots
Directionoftussockspread
Livingtillers
Deadtillers,
rhizomes
androots
B)
Living
fineroots
=2.5
t=10t=25t=50
=5 =10
y
xxx
y
y
Simulationresults
time
toxicityeffect
Ringformingplants
JournalofTheoreticalBiology2012
Simulationresults
tim
e
toxicity
effect
Ringformingplants
JournalofTheoreticalBiology2012
Vincenotetal.2017
Plants
Autotoxicity
Sesleriaappennina(Cartenietal.2012)
:)
www.ecoap.unina.it

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