Creative Biogene provides world-class packaging services for a variety of viral types using our QVirusTM platform. Creative Biogene's state-of-the-art facilities and highly experienced staff are available in order to assist in all areas of virus vector design and construction, as well as the generation of the virus in a quick turnaround. Our custom viral services are highlighted by a consistent high titer and large delivery viral counts.

1. Custom Viral Service
Creative Biogene
Email: info@creative-biogene.com
Website: http://www.creative-biogene.com

2. Creative Biogene provides world-class packaging services for a variety of viral
types using our QVirusTM platform. Creative Biogene's state-of-the-art facilities
and highly experienced staff are available in order to assist in all areas of virus
vector design and construction, as well as the generation of the virus in a quick
turnaround. Our custom viral services are highlighted by a consistent high titer
and large delivery viral counts.
Brief Introduction

3. Baculovirus ServiceAAV ServiceRetrovirus ServiceLentivirus Service
Content
Adenovirus Service
Viral vectors are a tool commonly used by molecular biologists to deliver genetic material into
cells. This process can be performed inside a living organism (in vivo) or in cell culture (in vitro).

4. Adenovirus Service
Adenovirus, a DNA virus, was first isolated in the 1950s in adenoid
tissue-derived cell cultures, hence the name. A large number of
acute respiratory, gastrointestinal and eye infections in humans are
caused by adenoviruses.
Recombinant adenoviruses are versatile and highly efficient tools used
for gene delivery and expression in mammalian cells. They provide a
versatile system for gene expression studies and therapeutic
applications in mammalian cells. Numerous biological features of
adenoviruses have made them the vector of choice for both in vitro
and in vivo applications. They can infect a broad range of cell types
with the highest efficiency.

5. Applications
01 Transient gene expression(cDNA, shRNA, miRNA etc.) in vivo and in vitro
02 Transient two or more transgenes expression from the same vector
03 Vectors containing two genes of interest
04 Vectors containing your gene of interest in the E1 region and a reporter gene in the E3 region

6. Lentivirus Service
Lentiviruses are very efficient at delivering genetic
materials to both dividing and non-dividing cells.
According to whether proviral DNA is formed in
transduced cells, lentiviruses can be further divided into
two groups: integrating lentivirus (ILV) and integrase-
deficient lentivirus (IDLV). ILV is first developed and
applied in scientific research and IDLV is constructed later
through genetic modification.

7. ILV and IDLV
ILV is the traditional lentivirus which can integrate the viral cDNA into its target cell genome to form proviral DNA.
The proviral DNA can replicate together with cell genome during cell dividing and exist in each new cell. This
feature makes ILV an ideal tool for stable cell line generation to allow long-term gene expression.
IDLV is derived from ILV via the use of integrase mutations. Unlike ILV, IDLV does not form proviral DNA. The viral
cDNA of IDLV exists as episomes which will be diluted and lost in the process of the cell dividing. IDLV can be used
for short-term gene expression.

8. Retrovirus is a highly efficient approach to deliver genes of interest for stable expression into a broad range of
dividing mammalian cells. Retroviral vectors are engineered to transfer and integrate target genetic material into
the genomes of host cells. Retroviruses have several distinct advantages over other vectors, especially when
permanent gene transfer is the preferred outcome. Some disadvantages of retroviruses are that they can only
transduce dividing cells, and that they integrate somewhat arbitrarily into genome which has the potential to
lead to oncogenesis.
Retrovirus Service

9. Retrovirus Service
 Synthesize your gene of interest/shRNA
 Clone gene of interest/shRNA into
appropriate retrovirus transfer vector
 Package retrovirus in HEK293T cells
 Retrovirus purification and titration

10. AAV Service
Adeno-associated virus (AAV) has been widely used by scientists to deliver exogenous genetic material into
cultured cells or live animal models. AAV can infect both dividing and non-dividing cells. Multiple AAV
serotypes (AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAV-6, AAV-8, AAV-9, AAV-DJ/8 and AAV-DJ) enable AAVs to
efficiently infect with broad specificity.
In general, recombinant AAVs are engineered to be persisting in an extrachromosomal state, which could
reduce the risk of mutation. Due to this feature, AAV DNA is lost through cell division but can be stable in
non-dividing cells.

11. ssAAV and scAAV
There are two types of AAV systems: Conventional Single-Stranded Adeno-
Associated Virus (ssAAV) and Self-Complementary Adeno-Associated Virus (scAAV).
Comparison of ssAAV and scAAV

12. Baculovirus Service
Baculoviruses are arthropod-specific viruses containing large double-stranded circular DNA
genomes of 80,000–180,000 bp. In recent years, they have been utilized for producing complex
eukaryotic proteins in insect cell cultures. There are several benefits of using baculovirus to express
target protein, such as the virus being able to package large inserts, the ability of post-translational
modifications, enhanced protein folding and function, high expression levels and safety.

13. Applications of baculovirus
01
Eukaryotic protein
processing
03
The development of
subunit vaccines
04
The construction of virus-like
particles (VLPs)
02
The production of
recombinant proteins

14. Features of Creative Biogene's services
Production of virus in a state-of-the-art
BSL-2 facility with robust quality control
in accordance with NIH Biosafety Level 2
criteria.
Using proprietary technologies that
are unique and highly efficient, we can
easily yield 108 to 109 TU/ml
lentivirus/retrovirus without
concentrating steps.
Creative Biogene has consolidated
its procedure of viral packaging
services as a standard production-
line resulting in a quick turnaround.
We will do anything in our power to
make sure your needs are met. We offer
plasmid amplification, custom vector
building, and large-scale production.

15. THANKS!
Creative Biogene