Dl ver of dos and donts

1. Clinical Laboratory’s
Do’s and Dont’s
BSMLS 2D
2013-2014

2. MEMBERS:
Bautista, Charles Christian H.
Brioso, Princess Michelle
Cabinian,Jaymilu
Cabral, Teresa Liway A.
Garcia, Maria Karissa A.
Gonzales, Jemmuel E.
Lontoc, Shelah Mae H.
Macatangay, Sophia
Tatlonghari, Edmon H.
Villacorta, Jon Vincent B.

3. Lab Safety

4. Why does it matter?
Safe working
protects:
You
Other lab workers
Cleaners
Visitors
Your work

5. Protecting yourself (PPE)
Fasten laboratory
coats
Don’t wear sandals or
open shoes
Tie back long hair

6. Gloves
Use the correct ones for
the job you will be doing
Remove gloves before
using instruments,
telephone, and leaving
the laboratory

7. Laboratory hygiene
Do not eat, drink or
smoke in a laboratory
Do not apply
cosmetics
Do not touch your
face, mouth or eyes
Do not suck pens or
chew pencils

8. Glassware Safety
Do not dispose
broken glassware in a
regular trashcan.
When pouring liquids
into glassware, make
sure the container
you are pouring into
is resting on a table
at least a hands
breadth from the
edge.

9. Spillages
Clear up spillage
promptly
Dispose of any
hazardous material as
toxic waste
“Messy workers are
usually poor workers!!”

10. Electrical Hazard
Always do a visual
check on electrical
equipment before
use, look for obvious
wear or defects

11. Waste Disposal
Do not put materials
down the drain or in with
normal waste unless
authorised to do so
Wear appropriate PPE
when handling clinical
waste

12. Chemical Safety
 Always add acid to water
 Pour from large containers
to smaller ones.
 Do not taste any chemical.
 Do not put your nose over
the container and inhale
the fumes.
Acid
Water

13. Fire Hazard
Use the smallest
quantities of
flammable solvents
practicable.
Do not use a Bunsen
burner in any area
where flammable
solvents are handled.

14. Risk of Infection
If exposed to a bio
hazardous specimen:
Immediately wash the
area with soap and H2O
Flush splashes to nose,
mouth or skin with H2O
Irrigate eyes with
clean H2O or saline
Perform background
tests

15. Aseptic Technique
Perform proper hand
washing

16. Reading Volume
Measurements
All readings must be
made with the eye on
level with the
meniscus to avoid
parallax error.

17. Pipetting techniques
Hold the pipet
between the thumb
and forefinger
Do not pipet using
your mouth, use a
rubber aspirator

18. Operating a Centrifuge
Do not remove the
cover of the
centrifuge until it
has come to a
complete stop
Make sure the loads
are balanced

19. Using microscope
Gently wipe the lens
with lens paper or
muslin cloth
Always carry the
microscope with one
hand supporting the
base and the other
hand around the arm.

20. Sharps Hazards
Always use
sterile,disposable
needle
Never recap a needle
Dispose sharps in
puncture-resistant,
leak-proof container

21. Order of Draw and Inversion
Always follow the
correct order of
draw of tubes and
tube inversion
Purple(8x)
Blue(3-4x)
Red glass (0x)
Red plastic (5x)

22. Syringes
Always transfer
immediately blood
drawn from syringe
into an evacuated
tube

23. Transporting samples to the
laboratory
Deliver samples to
the laboratory within
45 minutes and
centrifuge on arrival

24. Specimen Collection and
Handling Procedures
Allow samples to clot
fully before
centrifugation to
complete separation
of the cells and
serum
Protect bilirubin, Vit
A and carotene
samples from light

25. Laboratory Cleanliness
Do maintain good lab
cleanliness - it’s for
your own safety and
as well as for others!