1. IN VITRO EVLUATION OF THE CITREX ® MOLECULE AGAINST Listeria monocytogenes

14. Table 1. Test 1: Microorganism CITREX 400 ppm CITREX 200 ppm CITREX 100 ppm CITREX 50 ppm CITREX 25 ppm CITREX 12.5 ppm CITREX 6.25 ppm CITREX 3.125 ppm CITREX 1.562 ppm CITREX 0.781 ppm L. monocytogenes ATCC 19115 - - - - - + + + + + L. monocytogenes Wild Strain - - - + + + + + + +

15. Table 2. Test 2: Microorganism CITREX 400 ppm CITREX 200 ppm CITREX 100 ppm CITREX 50 ppm CITREX 25 ppm CITREX 12.5 ppm CITREX 6.25 ppm CITREX 3.125 ppm CITREX 1.562 ppm CITREX 0.781 ppm L. monocytogenes ATCC 19115 - - - - - + + + + + L. monocytogenes Wild Strain - - - + + + + + + +

16. Table 3. Test 3: Microorganism CITREX 400 ppm CITREX 200 ppm CITREX 100 ppm CITREX 50 ppm CITREX 25 ppm CITREX 12.5 ppm CITREX 6.25 ppm CITREX 3.125 ppm CITREX 1.562 ppm CITREX 0.781 ppm L. monocytogenes ATCC 19115 - - - - - + + + + + L. monocytogenes Wild Strain - - - + + + + + + +

17. Table 4. Result summary from all 3 assays Microorganism Minimum inhibitory concentration (ppm CITREX ®) L. monocytogenes ATCC 19115 25 L. monocytogenes Wild Strain 100

19. MIC RESULTS Microorganism CITREX 400 ppm CITREX 200 ppm CITREX 100 ppm CITREX 50 ppm CITREX 25 ppm CITREX 12.5 ppm CITREX 6.25 ppm CITREX 3.125 ppm CITREX 1.562 ppm CITREX 0.781 ppm L. monocytogenes ATCC 19115 - - - - - + + + + + L. monocytogenes Wild Strain - - - + + + + + + +

20. Blood Agar

21. Blood Agar

22. Blood Agar

23. Blood Agar

24. 6.- MINIMUM BACTERICIDAL CONCENTRATION 6.1.- RESULTS Microorganism Minimum bactericidal concentration (ppm) L. monocytogenes ATCC 50 L. monocytogenes Wild Strain 200

25. DIRECT IN VITRO VISUALIZATION OF THE CITREX® MOLECULE ACTION ON Listeria monocytogenes

47. TEST RESULTS PRIOR TO USING CITREX® ON MEAT Results with samples inoculated with L. monocytogenes , not disinfected with Citrex® SAMPLE RESULT Chicken meat Negative Beef Negative SAMPLE COUNT Chicken meat COUNTLESS Beef COUNTLESS

49. SAMPLE TYPE: CHICKEN MEAT TIME CONCENTRATION COUNT, CFUs 0 hours 600 ppm Countless 0 hours 400 ppm Countless 6 hours 600 ppm 10 X 10 2 6 hours 400 ppm 1.5 X 10 2 24 hours 600 ppm 8.4X10 1 24 hours 400 ppm 1.5 X 10 2 48 hours 600 ppm 23 x 10 2 48 hours 400 ppm 50 x 10 2

50. SAMPLE TYPE: BEEF TIME CONCENTRATION COUNT, CFUs 0 hours 600 ppm Countless 0 hours 400 ppm Countless 6 hours 600 ppm 4.7 X 101 6 hours 400 ppm 7 X10 1 24 hours 600 ppm 4 x 10 2 24 hours 400 ppm 51 x 10 2 48 hours 600 ppm 3 x 10 2 48 hours 400 ppm 30 x 10 2

60. SURFACE ANALYSIS AFTER A 6-HOUR ADAPTATION PERIOD POST-INOCULATION SURFACE ANALYSIS AFTER CLEANSING TREATMENT RESULTS AFTER 15 AND 30 MINUTES OF EXPOSURE TO CITREX ® AS A SURFACE DISINFECTANT AREA 1 COUNTLESS AREA 2 COUNTLESS AREA 1 48 CFUs/25 cm 2 AREA 2 36 CFUs/25cm 2 AREA 1: 400 ppm Citrex® 0 CFUs/25 cm 2 (no isolation) AREA 2: 600 ppm Citrex® 0 CFUs/25cm 2 (no isolation)