1. EVALUATION OF CITREX® ACTIVITY VS . A CHLORINE SOLUTION AS A DISINFECTANT DURING RAW SHRIMP PACKING PROCESS

7. 400 ppm Citrex Assay 1 Assay 2 Sample Mesophylls Sample Mesophylls Sample 1 3,000 Sample 1 400 Sample 2 4,000 Sample 2 4,000 Sample 3 1,800 Sample 3 3,000

8. 200 ppm Chlorine Assay 1 Assay 2 Sample Mesophylls Sample Mesophylls Sample 1 7,800 Sample 1 11,000 Sample 2 12,000 Sample 2 4,500 Sample 3 16,000 Sample 3 7,000

9. Farm shrimp 400 ppm Citrex Assay 1 Assay 2 Sample Mesophylls Sample Mesophylls Sample 1 520 Sample 1 280 Sample 2 310 Sample 2 1,500 Sample 3 5,400 Sample 3 1,100 Sample 4 2,700 Sample 4 950

10. 200 ppm Chlorine Assay 1 Assay 2 Sample Mesophylls Sample Mesophylls Sample 1 3,500 Sample 1 1,500 Sample 2 4,700 Sample 2 2,600 Sample 3 1,900 Sample 3 2,200 Sample 4 3,300 Sample 4 1,100

15. Coliforms - 400 ppm Citrex Assay 1 Assay 2 Sample Coliforms E. coli Sample Coliforms E. coli Sample 1 90 4 Sample 1 90 4 Sample 2 23 4 Sample 2 23 4 Sample 3 4 < 3 Sample 3 4 < 3

16. 200 ppm Chlorine Assay 1 Assay 2 Sample Coliforms E. coli Sample Sample E. coli Sample 1 200 21 Sample 1 200 9 Sample 2 200 3 Sample 2 200 3 Sample 3 200 40 Sample 3 200 9

17. Coliform Assays: Only farm shrimp, 400 ppm Citrex (March) Assay 1 Assay 2 Sample Coliforms E. coli Sample Coliforms E. coli Sample 1 11 < 3 Sample 1 9 < 3 Sample 2 7 <3 Sample 2 4 <3 Sample 3 3 < 3 Sample 3 11 3 Sample 4 21 3 Sample 4 9 3

18. Farm Shrimp - 200 ppm Chlorine Assay 1 Assay 2 Sample Coliforms E. coli Sample Coliforms E. coli Sample 1 11 <3 Sample 1 9 4 Sample 2 70 7 Sample 2 15 3 Sample 3 9 3 Sample 3 <3 <3 Sample 4 21 <3 Sample 4 23 11

19. GENUS DETERMINATION Salmonella spp.

23. Salmonella spp. Research 400 ppm Citrex Assay 1 Assay 2 Sample Salmonella spp. Sample Salmonella spp. Sample 1 Negative Sample 1 Negative Sample 2 Negative Sample 2 Negative Sample 3 Negative Sample 3 Negative

24. Sea shrimp - Salmonella 200 ppm chlorine Assay 1 Assay 2 ID Sample Salmonella spp. Sample Salmonella spp. Sample 1 POSITIVE Sample 1 POSITIVE Salmonella enteritidis Sample 2 POSITIVE Sample 2 POSITIVE Salmonella enteritidis Sample 3 Negative Sample 3 Negative

26. Sea shrimp – Vibrio: 400 ppm Citrex Assay 1 Assay 2 Sample Vibrio spp. Sample Vibrio spp. Sample 1 Negative Sample 1 Negative Sample 2 Negative Sample 2 Negative Sample 3 Negative Sample 3 Negative

27. Sea shrimp - Vibrio 200 ppm Chlorine Assay 1 Assay 2 Sample Vibrio spp. Sample Vibrio spp. Sample 1 Negative Sample 1 Negative Sample 2 Negative Sample 2 POSITIVE Sample 3 Negative Sample 3 Negative