2. • hidup dan berkembang di tempat ekstrem
• dapat mengembangkan sifat biokimia atau
fisiologis baru untuk beradaptasi
• mampu mensintesis enzim yang dapat
memfasilitasi stabilitas protein
• berperan dalam siklus geokimia di atmosfer,
menjaga keseimbangan kimia lingkungan,
membantu mengurangi GRK, dan detoksifikasi
bahan kimia berbahaya
Mikroorganisme
Ekstremofilik
bakteri termofilik
(Durvasula & Rao, 2018; Sani & Rathinam, 2018; Wolella & Tilahun, 2020)
6. Bioenergi Bioremediasi
Industri
(makanan,
detergen, dll.)
Kesehatan
Ekstremozim sebagai biokatalis memiliki sifat luar
biasa, seperti termostabilitas, adaptif dingin, dan
tunjangan osmotik, untuk memungkinkan mereka
digunakan dalam biorefinery, industri kimia,
bioremediasi, biomedis, dan pengendalian pencemaran
lingkungan.
7. produksi etanol (Hon et al., 2017).
Clostridium thermocellum
ATCC 31924
Cyanidium caldarium,
Galdieria sulphuraria Cryptococcus curvatus,
Lipomyces starkeyi
Bioenergi
Thermoanaerobacterium
saccharolyticum
meningkatkan produksi etanol
selulosa (Singh et al., 2018).
produksi biodiesel (Coker, 2016).
akumulasi lipid untuk bahan baku
biodiesel (Tsigie et al., 2011).
Cryptococcus curvatus. Source: Chattophadyay & Maiti (2021)
9. mendetoksifikasi ion logam
(Mergeay et al., 2003).
Pseudomonas simiae
Pseudomonas taiwanensis Anoxybacillus sp.
Bioremediasi
Ralstonia sp. CH34
mendegradasi minyak tanah
(Pham et al., 2014).
mendegradasi bensin
(Pham et al., 2014).
mendegradasi berbagai hidrokarbon (C8-
C22) pada 67 ℃ (Xia et al., 2015)
Anoxybacillus sp. Source: bacdive.dsmz.de/
10. menghasilkan beta karoten untuk
produk makanan dan obat (Kilic et al.,
2019).
Haloferax alexandrines
Bacillus halodurans Halorubrum lacusprofundi
Industri
Dunaliella salina
menghasilkan canthaxanthin
(Asker & Ohta, 2002).
produksi enzim untuk deterjen,
pemutihan pulp dan kertas, (Leemhuis
et al., 2010; Kumar & Satyanarayana,
2012; Vijayalaxmi et al., 2013 )
sintesis bahan kimia (Karan et al., 2013).
Bacillus halodurans. Source: www.researchgate.net/
11. reagen untuk PCR (Ishino & Ishino, 2014).
Sulfolobus sp.
Nostoc sp., Anabaena sp. Ectothiorhodospira halochloris
Kesehatan
Thermus aquaticus
menghasilkan peptida
antimikroba (Coker, 2016).
produksi syctonemin untuk obat dan
produk fotoproteksi (Gao & Garcia-
Pichel, 2011; Rastogi et al., 2015).
produksi ektoin untuk obat mata dan kulit
(Harishchandra et al., 2010).
Thermus aquaticus. Source: www.researchgate.net/
13. Kesimpulan
• Mikroorganisme ekstremofilik mewakili
sumber daya molekul dan organisme yang
sangat besar yang dapat dieksploitasi untuk
aplikasi yang tak terhitung banyaknya.
• Mikroorganisme ekstremofilik memiliki potensi
untuk memediasi katalisis dalam berbagai
kondisi operasi termasuk lingkungan yang
ekstrim dan meningkatkan laju katalitik.
• Beberapa di antaranya menghasilkan enzim
yang berkontribusi pada pemrosesan
makanan, formulasi deterjen, proses
bioremediasi, hingga obat.
14.
15. Referensi
• Asker, D., & Ohta, Y. (2002). Production of canthaxanthin by Haloferax alexandrinus under non-aseptic conditions and a simple, rapid method for its
extraction. Applied Microbiology and Biotechnology 2002 58:6, 58(6), 743–750. https://doi.org/10.1007/S00253-002-0967-Y
• Basen, M., Schut, G. J., Nguyen, D. M., Lipscomb, G. L., Benn, R. A., Prybol, C. J., Vaccaro, B. J., Poole, F. L., Kelly, R. M., & Adams, M. W. W. (2014). Single
gene insertion drives bioalcohol production by a thermophilic archaeon. Proceedings of the National Academy of Sciences of the United States of
America, 111(49), 17618–17623. https://doi.org/10.1073/PNAS.1413789111/SUPPL_FILE/PNAS.1413789111.SAPP.PDF
• Coker, J. A. (2016). Extremophiles and biotechnology: current uses and prospects. F1000Research, 5. https://doi.org/10.12688/F1000RESEARCH.7432.1
• Dalmaso, G. Z. L., Ferreira, D., & Vermelho, A. B. (2015). Marine Extremophiles: A Source of Hydrolases for Biotechnological Applications. Marine Drugs,
13(4), 1925. https://doi.org/10.3390/MD13041925
• De Vrije, T., De Haas, G., Tan, G. B., Keijsers, E. R. P., & Claassen, P. A. M. (2002). Pretreatment of Miscanthus for hydrogen production by Thermotoga
elfii. International Journal of Hydrogen Energy, 27(11–12), 1381–1390. https://doi.org/10.1016/S0360-3199(02)00124-6
• Durvasula, R., & Rao, D. V. S. (2018). Extremophiles : Nature’s Amazing Adapters. Extremophiles, 1–18. https://doi.org/10.1201/9781315154695-1
• Gao, Q., & Garcia-Pichel, F. (2011). Microbial ultraviolet sunscreens. Nature Reviews Microbiology 2011 9:11, 9(11), 791–802.
https://doi.org/10.1038/nrmicro2649
• Golyshina, O. V., Pivovarova, T. A., Karavaiko, G. I., Kondrat’eva, T. F., Moore, E. R. B., Abraham, W. R., Lünsdorf, H., Timmis, K. N., Yakimov, M. M., &
Golyshin, P. N. (2000). Ferroplasma acidiphilum gen. nov., sp. nov., an acidophilic, autotrophic, ferrous-iron-oxidizing, cell-wall-lacking, mesophilic
member of the Ferroplasmaceae fam. nov., comprising a distinct lineage of the Archaea. International Journal of Systematic and Evolutionary
Microbiology, 50(3), 997–1006. https://doi.org/10.1099/00207713-50-3-997/CITE/REFWORKS
• Harishchandra, R. K., Wulff, S., Lentzen, G., Neuhaus, T., & Galla, H. J. (2010). The effect of compatible solute ectoines on the structural organization of
lipid monolayer and bilayer membranes. Biophysical Chemistry, 150(1–3), 37–46. https://doi.org/10.1016/J.BPC.2010.02.007
16. Referensi
• Hon, S., Olson, D. G., Holwerda, E. K., Lanahan, A. A., Murphy, S. J. L., Maloney, M. I., Zheng, T., Papanek, B., Guss, A. M., & Lynd, L. R. (2017). The ethanol
pathway from Thermoanaerobacterium saccharolyticum improves ethanol production in Clostridium thermocellum. Metabolic Engineering, 42, 175–184.
https://doi.org/10.1016/J.YMBEN.2017.06.011
• Horikoshi, K. (1996). Alkaliphiles — from an industrial point of view. FEMS Microbiology Reviews, 18(2–3), 259–270. https://doi.org/10.1016/0168-
6445(96)00017-4
• Ishino, S., & Ishino, Y. (2014). DNA polymerases as useful reagents for biotechnology - The history of developmental research in the field. Frontiers in
Microbiology, 5(AUG), 465. https://doi.org/10.3389/FMICB.2014.00465/BIBTEX
• Karan, R., Capes, M. D., DasSarma, P., & DasSarma, S. (2013). Cloning, overexpression, purification, and characterization of a polyextremophilic β-
galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi. BMC Biotechnology, 13(1), 1–11. https://doi.org/10.1186/1472-6750-13-
3/FIGURES/7
• Kaur, A., Capalash, N., & Sharma, P. (2019). Communication mechanisms in extremophiles: Exploring their existence and industrial applications.
Microbiological Research, 221, 15–27. https://doi.org/10.1016/J.MICRES.2019.01.003
• Kilic, N. K., Erdem, K., & Donmez, G. (2019). Bioactive Compounds Produced by Dunaliella species, Antimicrobial Effects and Optimization of the Efficiency.
Turkish Journal of Fisheries and Aquatic Sciences, 19(11), 923–933. http://doi.org/10.4194/1303-2712-
v19_11_04%0Ahttp://www.trjfas.org/pdf/issue_19_11/1104.pdf
• Kumar, V., & Satyanarayana, T. (2012). Thermo-alkali-stable xylanase of a novel polyextremophilic Bacillus halodurans TSEV1 and its application in
biobleaching. International Biodeterioration & Biodegradation, 75, 138–145. https://doi.org/10.1016/J.IBIOD.2012.09.007
• Leemhuis, H., Kelly, R. M., & Dijkhuizen, L. (2010). Engineering of cyclodextrin glucanotransferases and the impact for biotechnological applications.
Applied Microbiology and Biotechnology, 85(4), 823–835. https://doi.org/10.1007/S00253-009-2221-3/TABLES/3
17. • Mergeay, M., Monchy, S., Vallaeys, T., Auquier, V., Benotmane, A., Bertin, P., Taghavi, S., Dunn, J., Van Der Lelie, D., & Wattiez, R. (2003). Ralstonia
metallidurans, a bacterium specifically adapted to toxic metals: towards a catalogue of metal-responsive genes. FEMS Microbiology Reviews, 27(2–3), 385–
410. https://doi.org/10.1016/S0168-6445(03)00045-7
• Nies, D. H., & Nies, D. H. (2000). Heavy metal-resistant bacteria as extremophiles: molecular physiology and biotechnological use of Ralstonia sp. CH34.
Extremophiles 2000 4:2, 4(2), 77–82. https://doi.org/10.1007/S007920050140
• Pham, V. H. T., Kim, J., & Jeong, S.-W. (2014). Enhanced isolation and culture of highly efficient psychrophilic oil-degrading bacteria from oil-contaminated
soils in South Korea. Journal of Environmental Biology, 35, 1145–1149.
• Poontawee, R., Yongmanitchai, W., & Limtong, S. (2017). Efficient oleaginous yeasts for lipid production from lignocellulosic sugars and effects of
lignocellulose degradation compounds on growth and lipid production. Process Biochemistry, 53, 44–60. https://doi.org/10.1016/J.PROCBIO.2016.11.013
• Rastogi, R. P., Sonani, R. R., & Madamwar, D. (2015). Cyanobacterial Sunscreen Scytonemin: Role in Photoprotection and Biomedical Research. Applied
Biochemistry and Biotechnology 2015 176:6, 176(6), 1551–1563. https://doi.org/10.1007/S12010-015-1676-1
• Sani, R. K., & Rathinam, N. K. (2018). Extremophilic microbial processing of lignocellulosic feedstocks to biofuels, value-added products, and usable power. In
Extremophilic Microbial Processing of Lignocellulosic Feedstocks to Biofuels, Value-Added Products, and Usable Power. https://doi.org/10.1007/978-3-319-
74459-9
• Singh, N., Mathur, A. S., Gupta, R. P., Barrow, C. J., Tuli, D., & Puri, M. (2018). Enhanced cellulosic ethanol production via consolidated bioprocessing by
Clostridium thermocellum ATCC 31924☆. Bioresource Technology, 250, 860–867. https://doi.org/10.1016/J.BIORTECH.2017.11.048
• Śpibida, M., Krawczyk, B., Olszewski, M., & Kur, J. (2017). Modified DNA polymerases for PCR troubleshooting. Journal of Applied Genetics, 58(1), 133–142.
https://doi.org/10.1007/S13353-016-0371-4/TABLES/3
• Tsigie, Y. A., Wang, C. Y., Truong, C. T., & Ju, Y. H. (2011). Lipid production from Yarrowia lipolytica Po1g grown in sugarcane bagasse hydrolysate. Bioresource
Technology, 102(19), 9216–9222. https://doi.org/10.1016/J.BIORTECH.2011.06.047
• Vijayalaxmi, S., Prakash, P., Jayalakshmi, S. K., Mulimani, V. H., & Sreeramulu, K. (2013). Production of Extremely Alkaliphilic, Halotolerent, Detergent, and
Thermostable Mannanase by the Free and Immobilized Cells of Bacillus halodurans PPKS-2. Purification and Characterization. Applied Biochemistry and
Biotechnology 2013 171:2, 171(2), 382–395. https://doi.org/10.1007/S12010-013-0333-9
• Wolella, E. K., & Tilahun, B. (2020). Isolation and Characterization of Extremophilies from Shalla / Abidjata Hot Springs , Ethiopia. Acta Scientific
Microbiology, 3(2), 1–5.
• Xia, W., Dong, H., Zheng, C., Cui, Q., He, P., & Tang, Y. (2015). Hydrocarbon degradation by a newly isolated thermophilic Anoxybacillus sp. with bioemulsifier
production and new alkB genes. RSC Advances, 5(124), 102367–102377. https://doi.org/10.1039/C5RA17137G
Referensi