Norman E. Borlaug International Agricultural Science and Technology Fellowship Program,

1. Wheat Disease Research Department
Plant Pathology Research Institute
ARC, Egypt
“ Rusts never sleep "
Norman E. Borlaug International Agricultural Science and Technology
Fellowship Program,
2013, Egypt
Identification of wheat rusts resistance genes in some
newly-developed Egyptian wheat cultivars by biotechnology
component " molecular markers"

3. Welcome to Michigan
Lansing
Michigan Capitol Building

4. Agriculture in Michigan
 2nd most diverse agriculture in the U.S
 Major crops – Maize, Soybean, Sugarbeet, Beans, Wheat,
Alfalfa, Cucumber, Potato, Tomato, Asparagus, Onion,
Apple, Blueberry, Peach, Cherry, and Strawberries
 Vibrant Livestock sector – Dairy
 Agriculture contributes more that $70 billion to
Michigan economy

5. MSU: A beautiful campus during
four seasons of the year….
SPRING SUMMER
AUTUMN WINTER

7. 2013 Course – 10 Countries Represented
 Algeria
 Egypt
 Ethiopia
 China
 India
 Indonesia
With diverse backgrounds….
17 participants from 10 countries
 Nigeria
 Turkey
 Uganda
 USA

8. Molecular Plant Breeding short course
 Objective: Provide an overview on the use of
molecular markers in plant breeding

10. Molecular Plant Breeding Short Course
Organized by
The World Technology Access Program in collaboration with Plant Breeding, Genetic and
Biotechnology Program
August 25-30, 2013
learned new ideas on :
• Fundamental molecular biology techniques and marker assisted
breeding
• Marker trait associations analyses for qualitative and
quantitative traits.
• Marker assisted breeding.
• DNA sequencing, SNP markers and genomics guided breeding.
• Computer software such as R program.
• Visits to plant breeding programs at MSU.
• New tools for molecular breeding.

11. Tuesday, Aug 27
 DNA sequencing and new markers!
‒ Hands on activities in DNA sequencing
‒ Next generation sequencing technologies
‒ Discovery of SNP markers
 Genomics guided plant breeding
 Visits to breeding programs at MSU

12. Wednesday, Aug 28
 Hands on sessions in linkage map construction
‒ Using SNP marker data for developing a linkage
map using the Joinmap software
 Introduction and hands-on session on freely
available computer software programs for
linkage mapping – R software

14. Staff of wheat breeding and genetics Lab.

15. Eric Olson, Lab. “wheat breeding and genetics “
- Lab. Activities :
-Test the germination rate of seed from
CYMMIT.
- Collection tissue for DNA extraction.
- Extract DNA.
- PCR (Genotyping).
- Data analysis (presence / absence) of
resistance genotype (Lr34+Yr18, Yr17 and
Sr2)

20. Greenhouse Activities :
-Transplant lab germinated seeds to cultivate
in the greenhouse.
-Collect tissue – to be used for DNA extraction.
-Collection of urediospores and inoculation of
wheat with stem rust - then incubation.
- Cross between plants having Lr34 resistance
gene and susceptible plants.
Lab. Meeting
- weekly meeting for discussion of scientific
publications

23. Phenotyping
Genotyping
Genotyping in Lab.
Using leaf tip necrosis as a phenotypic marker to
predict the presence of durable rust resistance gene
pair Lr34/Yr18 in wheat*
*Syed et al., 2011, J. Gen. Plant Pathol 77:174-177
Phenotyping in field

24. Fig. CAPS marker csSr2 tested on diverse wheats and run on a agarose
gel. Lanes (1) Chinese Spring; (2) Hope; (3) Chinese Spring 3BS
deletion line (FLM 0.86); (4) Marquis; (5) Redman; (6) Kenya Plume;
(7) Thatcher; (8) Purplestraw; (9) Yaroslav emmer. The arrow shows
the fragment which is associated with Sr2. The rust phenotype is
represented as R (resistant) and S (susceptible)
R. Mago • G. et al., (2011). An accurate DNA marker assay for stem rust resistance gene Sr2 in wheat. Theor Appl
Genet 122:735–744.

25. Lr34
status
Specific markers
Cross
No.
cssfr5
cssfr2
cssfr1
-/?
0/?
1/1
0/0
103 × 266
-/-
0/0
0/1
0/0
103 × 076
-/-
0/0
0/1
0/0
103 × 050
+/-
?/0
0/1
1/0
280 × 067
-/?
0/?
1/0
0/0
081 × 274
?/?
?/?
0/0
0/0
274 × 273
Het/?
0/?
1/0
1/0
073 × 273
+/?
0/?
1*/1
1/0
054 × 229
+/-
0/0
1*/1
1/0
054 × 089
The selected genotypes, were crossed which having
leaf rust resistance Lr34

26. Lr34
status
Specific markers
Cross
No.
cssfr5
cssfr2
cssfr1
+/?
0/?
1*/0
1/0
054 × 128
+/?
0/?
1*/0
1/0
048 × 134
+/?
0/?
1*/1
1/0
048 × 226
?/-
?/0
1/1
0/0
222 × 067
?/het
?/0
1/1
0/1*
222 × 021
?/?
?/?
1/0
0/0
222 × 189
?/?
?/?
1/0
0/0
222 × 253
+/?
0/?
1*/1
1/0
054 × 229
Het/-
00
1*/1
1*/0
049 × 033
Het/-
00
1/1
1*/0
008 × 033
Het/?
0/?
1*/1
1*/0
049 × 226
Het/-
0/0
1/1
1*/0
051 × 033
Het/?
0/?
1*/1
1*/0
049 × 225

28. Dear Atef and Yasmina,
It was a pleasure to work with you both at MSU and I hope the experience was valuable. In addition
to the information on Yr17 status of the lines in your crosses, I will send Yr17 data on the remaining
population. The PCR products from the other two plates are currently in the freezer and I will run the
gels on Monday. If it will fit with your schedule I will send the data to you Monday afternoon (US).
Our plan to evaluate progeny from crosses you have made is underway. During the greenhouse
season, we have grown the F1 plants from 24 crosses (.xlsx attached). These have been harvested
and are currently being processed. To carry on our project, I see two potential routes to follow:
1.) Divide the F2 seed from each of the 24 crosses in half and send F2 seed to each of you. Then,
these may be grown and selected under Algerian and Egyptian conditions by conventional breeding
approaches.
2.) Alternatively, We could genotype all F2 seed for the presence of Yr17 and Lr34 and identify F2
plants with Yr17, Lr34 and Yr17+Lr34. Then, we could grow the F2 at MSU and then send you the
F2:3 seed to evaluate for stripe rust resistance under field conditions in Algeria and Egypt.
With route 2 we may be able to generate a publication as we discussed, on the pleiotropic effects of
Lr34 on major gene stripe rust resistance. I have not yet seen this documented in the literature as
with stem rust (paper on Lr34 effects on stem rust major genes attached). Also, this could provide
breeding programs with valuable germplasm developed by your efforts.
I look forward to hearing your thoughts on the future directions of our collaboration.
All the best,
Eric

29. Participate on 2013 World Food Prize

31. Charity kawira Mutegi
Kenya
The person most in charge of your success is
yourself. "Achieve Anything“*
- Get motivation: and Stay motivated
- Linkage with extra scientist
- Good lesson and education
- Define, pursue and celebrate personal success
"Whatever you can do, or dream you can begin it.
Boldness has
genius, power and magic in it."--Goethe
- . . . that's certainly my plan!
*Achieve Anything in Just One Year: Be Inspired Daily to Live Your Dreams and Accomplish Your Goals Paperback
by Jason Harvey

33. Dear: Atef A. Shahin
ARC
EGYPT
Sanjaya Rajaram, Former CIMMYT Wheat Program Director Wins 2014 World Food Prize
CIMMYT is delighted to announce that the World Food Prize 2014 has been awarded to distinguished
wheat breeder Dr. Sanjaya Rajaram for his achievements in plant research and food production. Rajaram
- known affectionately as “Raj”— led bread wheat breeding research at CIMMYT for more than three
decades. His leadership and commitment to wheat improvement resulted in the release of more than 480
varieties of bread wheat with increased yield potential, wide adaptation, and resistance to important
diseases and stresses. Rajaram’s wheats are grown on some 58 million hectares worldwide and
approximately 30 million hectares in South Asia. His new varieties increased the yield potential of wheat
by 20 to 25 percent. He also led efforts at CIMMYT to apply the concept of durable resistance to rust—the
most damaging disease of wheat worldwide. His accomplishments include training or mentoring more
than 700 scientists from dozens of developing countries.
Rajaram was nominated for the World Food Prize by Dr. Thomas Lumpkin, Director General and Dr. Hans
Braun, Director Global Wheat Program at CIMMYT, with support from National Agricultural Research
Institutes around the world. Congratulations Raj from all the staff at CIMMYT! We continue to be inspired
by your work which has benefited millions of farmers and consumers all over the world.
http://www.cimmyt.org/en/2014-world-food-prize
CIMMYT Global Wheat Program

35. Future work :
I am too much interest in developing the work on wheat rusts in
Egypt either in the greenhouse or in laboratory, So I hope to get
an international project, participated by more one organization .
- When I come back to my country I will transfer these
knowledge to my colleagues which will reflect benefits to the
growers.
- Establishment biotechnology laboratory serves practical in the
field of specialist in diseases of wheat.
- Continuous communication with keep cooperation between the
USDA, MSU, Eric lab. … etc. and ARC in Egypt.
There is nothing like a Dream to create the
future …Victor Hugo

36. ACKNOWLEDGMENTS
- Ramzy Sherief
- Karen Uetrecht
- Karim Maredia
- Cholani Weebadde
- Eric Olsen
- Andrew Wiersma
-Yasmina Haddadi
-Beth Brisco
-Rania Ahmed
-Gemechu Keneni
-Tariku Tolera
-Hans Raj
- Jane Kim

38. ‫االستماع‬ ‫لحسن‬ ‫شكرا‬
Contact : a.a.shahin@hotmail.com
June, 30, 2014