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Plant Development
PS452
Feng Chen
Department of Plant Sciences
University of Tennessee
01/21/2014
Plant Growth: an irreversible change in the size of a cell, organ or
whole organism.
Differentiation: Cells taking on specialized form and function.
Plant Development: the orderly and progressive change from seed
germination through juvenility, maturity, flowering and fruiting.
Seed
Seedling
Mature plants
Flowering
Fruiting
Plant Life Cycle
(1) How is a seed formed? (2) How does a seed
become a seedling?
(3) How is shoot formed?
(4) How is root formed?
(5) How is a flower
formed?
(1) How is a seed formed?
(2) How does a seed become a seedling?
(3) How is shoot is formed?
(4) How is root is formed?
(5) How is a flower formed?
Flower Structure
Figure 4.2
Male and Female Gametophyte
Gametophyte – embryo sac
Gametophyte – anther
Gamete – egg
Gamete – two sperm cells (in pollen grain or tube)
Male
Female
21-14
Anther
Tapetum
(nutritive)
Epidermis
Raven et al., 1999; Biology of Plants
Anther
(lily)
Pollen sac Pollen mother cells
(Microsporocytes)
Tetrad
(n)
Free microspores
(n)
Mature pollen
(n)
Nucleus of
vegetative cell
Generative cell
Pollen mother cell (Microsporocyte)
(2n)
Meiosis
Pollen Development
“‘diploid’”
“‘haploid’”
Ovule Development
micropyle
funiculus
outer
integument
inner
integument nucellus 20.8
Esau, 1977; Anatomy of Seed Plants
embryo
sac
Megasporogenesis
(2n) (n) (n) (n)
Meiosis
Megasporocyte
(mother cell)
“Megasporogenesis”
Egg Cell Differentiation
Buchanan et al., 2000’ Biochemistry and Molecular Biology of Plants
Haploid Egg
Antipodal cells
Egg cell
Synergid
cells
Central nuclei
ovule
stigma
Pollen tube
Fertilization
Fertilization
Double Fertilization
Antipodal cells
Central nuclei
Egg cell
Synergid cell
Sperm nuclei
Endosperm
Embryo
Figure 4.4
Embryogenesis
Simplified Structure of A Mature Seed
Seed coat
Embryo
Endosperm
Seed coat is dead tissue.
It protects everything
inside it.
Embryo is a minute
plant .
Endosperm provides energy
for seed germination and
early seedling growth.
(1) How is a seed formed?
(2) How does a seed become a seedling?
(3) How is shoot formed?
(4) How is root formed?
(5) How is a flower formed?
Dry seeds Imbibed seeds
Water Uptake: the First Event in Germination
Bewley, Plant Cell
Events Occur during Seed Germination
Water Relations and Seed Germination
High water availability
Medium water availability
Low water availability
Temperature and Seed Germination
Some Seeds Require Light for Germination
Dark Dark
Light
Tomato Seed Anatomy
Endosperm
Seed coat
Embryo
0.5 mm
Embryo
Endosperm
Embryo
Endosperm
Seed Coat Seed Coat
Embryo
Endosperm
Growth Potential
Constraint
Seed Coat
Involvement of Expansin Genes in Seed Germination
LeEXP4
Dissecting seed
LeEXP8
(1) How is a seed formed?
(2) How does a seed become a seedling?
(3) How is shoot formed?
(4) How is root formed?
(5) How is a flower formed?
Figure 4.1
Formation of SAM
Taiz and Zeiger, Plant Physiology
CZ: central zone; PZ: peripheral zone; RZ: rib zone
Shoot Apical Meristem (SAM)
Taiz and Zeiger, Plant Physiology
Leaf Formation: Leaf Primordium
Figure 4.7
Leaf Anatomy
Taiz and Zeiger, Plant Physiology
From: PM Ray, “The Living Plant”
Apical dominance is a
phenomenon in which the
apical bud tends to
“dominate” stem growth in
the sense that all of the
axillary buds immediately
below it do not grow out to
form branches. Thus the
stem grows tall, not wasting
resources by growing wide.
The idea is that auxin
produced in the apical bud is
transported down the stem
and suppresses the
outgrowth of the lateral
(axillary) buds. If the tip is
cut off the auxin source is
removed and the buds begin
to develop into branches.
(1) How is a seed formed?
(2) How does a seed become a seedling?
(3) How is shoot formed?
(4) How is root formed?
(5) How is a flower formed?
Root Systems
Taproot system: characterized by
having one main root (the taproot)
from which smaller branch roots
emerge. When a seed germinates,
the first root to emerge is the radicle,
or primary root. In conifers and
most dicots, this radicle develops
into the taproot.
Fibrous root system: characterized by
having a mass of similarly sized roots. The
radicle from a germinating seed is short lived
and is replaced by adventitious roots.
Adventitious roots are roots that form on
plant organs other than roots. Most monocots
have fibrous root systems.
Root tip has 4 developmental zones
Root cap: Protects RAM and push
Meristematic zone: Primary root
Elongation zone: Rapid cell
elongation, rate of division
decreases with distance from
meristem
Maturation zone: Cells get their
mature differentiated features.
No lateral organs produced from apical
meristem to avoid hindrance in soil
penetration
Branch roots arise from non
growing region
Root System development
Cells of the root epidermis develop projections called root hairs. These
elongate by “tip growth” and increase surface area for water and mineral
uptake. Root hairs are found away from the root tip, in the region of
maturation.
Note that the root hair develops as an
outgrowth from individual epidermal
cells; that is, the root hair is not a cell
separate from the epidermal cell. The
Figure shows (bottom to top) four
stages of root hair development: cell
specification, root hair initiation, tip
growth, and maturation.
(1) How is a seed formed?
(2) How does a seed become a seedling?
(3) How is shoot formed?
(4) How is root formed?
(5) How is a flower formed?
Flower Structure
Internal factors
Phase change
Hormones
External factors
Light
Temperature
Total light radiation
Water availability
Cues for Flower Evocation
Flower Evocation: The events occurring in the shoot apex that
specifically commit the apical meristem to produce flowers.
Vegetative SAM Reproductive SAM
Formation of Floral Meristems
Genetic Control of Flower Development
Taiz and Zeiger, Plant Physiology
Three Types of Genes Control Floral Identify
1. Gene A activity controls the first and second whorls
2. Gene B activity controls the second and third whorls
3. Gene C activity controls the third and fourth whorls.
ABC Model for Flower Development
Taiz and Zeiger, Plant Physiology
Figure 4.8
Mutations in Floral Organ Identify Genes
Seed
Seedling
Mature plants
Flowering
Fruiting
Plant Life Cycle
(1) How is a seed formed? (2) How does a seed
become a seedling?
(3) How is shoot formed?
(4) How is root formed?
(5) How is a flower
formed?

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Plant Growth and Development Processes

  • 1. Plant Development PS452 Feng Chen Department of Plant Sciences University of Tennessee 01/21/2014
  • 2. Plant Growth: an irreversible change in the size of a cell, organ or whole organism. Differentiation: Cells taking on specialized form and function. Plant Development: the orderly and progressive change from seed germination through juvenility, maturity, flowering and fruiting.
  • 3. Seed Seedling Mature plants Flowering Fruiting Plant Life Cycle (1) How is a seed formed? (2) How does a seed become a seedling? (3) How is shoot formed? (4) How is root formed? (5) How is a flower formed?
  • 4. (1) How is a seed formed? (2) How does a seed become a seedling? (3) How is shoot is formed? (4) How is root is formed? (5) How is a flower formed?
  • 7. Male and Female Gametophyte Gametophyte – embryo sac Gametophyte – anther Gamete – egg Gamete – two sperm cells (in pollen grain or tube) Male Female
  • 8. 21-14 Anther Tapetum (nutritive) Epidermis Raven et al., 1999; Biology of Plants Anther (lily) Pollen sac Pollen mother cells (Microsporocytes)
  • 9. Tetrad (n) Free microspores (n) Mature pollen (n) Nucleus of vegetative cell Generative cell Pollen mother cell (Microsporocyte) (2n) Meiosis Pollen Development “‘diploid’” “‘haploid’”
  • 10. Ovule Development micropyle funiculus outer integument inner integument nucellus 20.8 Esau, 1977; Anatomy of Seed Plants embryo sac
  • 11. Megasporogenesis (2n) (n) (n) (n) Meiosis Megasporocyte (mother cell) “Megasporogenesis”
  • 12. Egg Cell Differentiation Buchanan et al., 2000’ Biochemistry and Molecular Biology of Plants
  • 13. Haploid Egg Antipodal cells Egg cell Synergid cells Central nuclei
  • 15. Double Fertilization Antipodal cells Central nuclei Egg cell Synergid cell Sperm nuclei Endosperm Embryo
  • 17. Simplified Structure of A Mature Seed Seed coat Embryo Endosperm Seed coat is dead tissue. It protects everything inside it. Embryo is a minute plant . Endosperm provides energy for seed germination and early seedling growth.
  • 18. (1) How is a seed formed? (2) How does a seed become a seedling? (3) How is shoot formed? (4) How is root formed? (5) How is a flower formed?
  • 19. Dry seeds Imbibed seeds Water Uptake: the First Event in Germination
  • 20. Bewley, Plant Cell Events Occur during Seed Germination
  • 21. Water Relations and Seed Germination High water availability Medium water availability Low water availability
  • 22. Temperature and Seed Germination
  • 23. Some Seeds Require Light for Germination Dark Dark Light
  • 27. Involvement of Expansin Genes in Seed Germination LeEXP4 Dissecting seed LeEXP8
  • 28. (1) How is a seed formed? (2) How does a seed become a seedling? (3) How is shoot formed? (4) How is root formed? (5) How is a flower formed?
  • 30. Formation of SAM Taiz and Zeiger, Plant Physiology
  • 31. CZ: central zone; PZ: peripheral zone; RZ: rib zone Shoot Apical Meristem (SAM) Taiz and Zeiger, Plant Physiology
  • 32. Leaf Formation: Leaf Primordium
  • 34. Leaf Anatomy Taiz and Zeiger, Plant Physiology
  • 35. From: PM Ray, “The Living Plant” Apical dominance is a phenomenon in which the apical bud tends to “dominate” stem growth in the sense that all of the axillary buds immediately below it do not grow out to form branches. Thus the stem grows tall, not wasting resources by growing wide. The idea is that auxin produced in the apical bud is transported down the stem and suppresses the outgrowth of the lateral (axillary) buds. If the tip is cut off the auxin source is removed and the buds begin to develop into branches.
  • 36. (1) How is a seed formed? (2) How does a seed become a seedling? (3) How is shoot formed? (4) How is root formed? (5) How is a flower formed?
  • 37. Root Systems Taproot system: characterized by having one main root (the taproot) from which smaller branch roots emerge. When a seed germinates, the first root to emerge is the radicle, or primary root. In conifers and most dicots, this radicle develops into the taproot. Fibrous root system: characterized by having a mass of similarly sized roots. The radicle from a germinating seed is short lived and is replaced by adventitious roots. Adventitious roots are roots that form on plant organs other than roots. Most monocots have fibrous root systems.
  • 38. Root tip has 4 developmental zones Root cap: Protects RAM and push Meristematic zone: Primary root Elongation zone: Rapid cell elongation, rate of division decreases with distance from meristem Maturation zone: Cells get their mature differentiated features. No lateral organs produced from apical meristem to avoid hindrance in soil penetration Branch roots arise from non growing region Root System development
  • 39. Cells of the root epidermis develop projections called root hairs. These elongate by “tip growth” and increase surface area for water and mineral uptake. Root hairs are found away from the root tip, in the region of maturation. Note that the root hair develops as an outgrowth from individual epidermal cells; that is, the root hair is not a cell separate from the epidermal cell. The Figure shows (bottom to top) four stages of root hair development: cell specification, root hair initiation, tip growth, and maturation.
  • 40. (1) How is a seed formed? (2) How does a seed become a seedling? (3) How is shoot formed? (4) How is root formed? (5) How is a flower formed?
  • 42. Internal factors Phase change Hormones External factors Light Temperature Total light radiation Water availability Cues for Flower Evocation Flower Evocation: The events occurring in the shoot apex that specifically commit the apical meristem to produce flowers.
  • 43. Vegetative SAM Reproductive SAM Formation of Floral Meristems
  • 44. Genetic Control of Flower Development Taiz and Zeiger, Plant Physiology
  • 45. Three Types of Genes Control Floral Identify 1. Gene A activity controls the first and second whorls 2. Gene B activity controls the second and third whorls 3. Gene C activity controls the third and fourth whorls.
  • 46. ABC Model for Flower Development Taiz and Zeiger, Plant Physiology
  • 47.
  • 48.
  • 49. Figure 4.8 Mutations in Floral Organ Identify Genes
  • 50. Seed Seedling Mature plants Flowering Fruiting Plant Life Cycle (1) How is a seed formed? (2) How does a seed become a seedling? (3) How is shoot formed? (4) How is root formed? (5) How is a flower formed?