My final project at "Instrumental Analysis Laboratory" was about CE-PL

1. Instrumental Analysis
Laboratory
Fall 2014
Final Project
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2. Introduction to CE-PL
Capillary Electrophoresis-Photoluminescence
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3. Capillary Electrophoresis
Abbreviated as:
CE: Capillary Electrophoresis
CZE: Capillary Zone Electrophoresis
HPCE: High-Performance Capillary Electrophoresis
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4. ELECTROPHORESIS:
Electrophoresis is the motion of dispersed particles relative to a fluid
under the influence of a spatially uniform electric field
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5. CE Apparatus:
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6. Capillary Structure and
Electroosmotic Flow:
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7. Injection:
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1. Electrokinetic Method(Used for Ionic Solutes)
2. Hydrodynamic Methods(Used for Neutral Solutes)
• Using Gravity Flow
• Negative Pressure to the Outlet end of Column
• Positive Pressure to the Sample Solution

8. Detector:
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• UV-vis Absorbance
• Photoluminescence
• Electrochemical Methods
• Mass Spectrometry
• Potentiometric
• Conductometric
• Amperometric
• Laser Induced Fluorescence(LIF)
the most sensitive detection method with detection limits about nM
• Indirect LIF

9. CE vs. LC
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• High Efficiency
• Short Analysis Time
• Simple Apparatus
• Ease in Changing the Separation Buffer
• Small Sample and Separation Buffer Volumes
• High Selectivity(through changing the buffer additives)

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Oregongreen 488 carboxylic acid, succinimidyl ester(OG-SE)
Most of the analytes do not have native fluorescence, therefor,
fluorescent probe molecules should attach to the molecules

12. Experimental:
Samples were collected every hour
from fermentation broth.(for 210h)
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Electropherogram of one of the samples `
The buffer was prepared from
sodium tetraborate
To enhance the separation between
amino acids sodium dodecyl sulfate(SDS)
and 18-crown-6 ether was added

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Results:

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Thank You For Your Consideration
Ali Amiri Naeini