presentation on : Drug protein binding prepared by Ali Asraf Sohel

1. Presentation on: Drug protein binding.
Presentation by: ALI ASRAF SOHEL
ID:2015000300008
Southeast university

2. Overview
 Impact of the altered protein
binding
 Distribution and Plasma Protein
Binding
 Measuring Plasma Protein
Binding
 Conclusion

3. Impact of the altered protein
binding
►Only the unbound fraction of the drug
undergoes metabolism in the liver and
other tissues
► As the drug dissociates from the protein
more and more drug undergoes metabolism
►Changes in the levels of free drug change
the volume of distribution

4. ►Rapidly undergo metabolism, clearance is
dependent on the hepatic blood flow.
►Slowly undergo drug directly change the
clearance of the drug.
►If the amount of plasma protein is
decreased (such as liver disease, renal
disease), there would also be a higher
fraction unbound.

5. Distribution and Plasma Protein
Binding
►The distribution of a drug is often
measured as a volume of distribution.
►It is also a measure of the fluid volume.
►It is important to note that the
measured volumes are not actual
physical volumes.

6. Typical Liquid Volumes For A
70kg Man Are (% And L)
 Total Water: 60%, 42 L
 Intracellular Volume: 40%, 28L
 Extracellular Volume: 20%, 14L
 Plasma Volume: 4%, 3L
 Blood Volume: 8%, 5.5L

7. Measuring Plasma Protein
Binding
►Equilibrium dialysis is the most widely
accepted method for assessing plasma
protein binding.
►But is a relatively slow process, from 4-24
hours at 37 C.
►For very highly bound compounds it can
be difficult to measure the concentration of
unbound drug.

8. Conclusion
Overall we can say it drug protein binding is
very important for drug distribution. In
every step of drug distribution it plays an
important role.