Describe the purpose for carrying out a DNAase Hypersensitivity assay. How do you analyze and interperet the DNAse Hypersensitivity assay? Solution DNase I hypersensitivity analysis is a powerful method to analyze chromatin structure and identify genomic regulatory elements. in this technique Euchromatin regions are associated with active form (open form so more accesible to various DNAase) and can be identified by their hypersensitivity to nuclease digestion. Hypersensitive sites can be identified by DNase hypersensitivity assays, in which intact, isolated nuclei are subjected to limited digestion with the enzyme DNase I. DNase I cleavage sites are identified by restriction digestion followed by Southern blotting using radiolabeled genomic fragments to probe a specific genomic region of interest. so in undigested lane you would be able to see only one band where as in digested one more than one band. now a days this technique combined with second genration sequencing for better understanding..

1. Describe the purpose for carrying out a DNAase Hypersensitivity assay. How do you analyze
and interperet the DNAse Hypersensitivity assay?
Solution
DNase I hypersensitivity analysis is a powerful method to analyze chromatin structure and
identify genomic regulatory elements. in this technique Euchromatin regions are associated with
active form (open form so more accesible to various DNAase) and can be identified by their
hypersensitivity to nuclease digestion. Hypersensitive sites can be identified by DNase
hypersensitivity assays, in which intact, isolated nuclei are subjected to limited digestion with the
enzyme DNase I. DNase I cleavage sites are identified by restriction digestion followed by
Southern blotting using radiolabeled genomic fragments to probe a specific genomic region of
interest.
so in undigested lane you would be able to see only one band where as in digested one more than
one band. now a days this technique combined with second genration sequencing for better
understanding.