Plant Bioreactor

1. SEMINAR
ON
TRANSGENIC PLANTS AS
BIOREACTOR
PRESENTED BY
ADITYA
M.Sc. III SEM
BIOTECHNOLOGY

2. INTRODUCTION
• Genetically modified plants can be manipulated to act
as bioreactors to produce wide range of biologically
important compounds.
• These include carbohydrate, lipids ,protein, besides
the secondary products.
• The commercial products of plants are useful for
industries or improving the human and animal
health.

3. What are the transgenic plants &
bioreactor
• Plant genetic transformation technology basically
deals with the transfer of desirable genes from one
plant species to another with subsequent integration
and expression of foreign gene in host genome.
• The term trans gene is used to represent the
transferred gene.
• The genetically transformed new plants are regarded
as transgenic plants.

4. BIOREACTOR
• The heart of fermentation technology is bioreactor.
• A bioreactor is basically a device in which the
organism (cells) are cultivated and motivated to from
the desired products.

5. Plants as bioreactor
• Transgenic plants have significant potential in the bio-
production of complex human therapeutic agents due
to ease of genetic manipulation, lack of potential
contamination with human pathogen.
• Plant cell are an attractive horologes expression host
for bimolecular production specially protein.
• The terms molecular farming or metabolic engineering
of plants are also used to synthesis of commercial
products.

6. Comparison with other production system
• Low cost .
• Post translational modification.
• Storage facilities
• Low upstream production cost.

7. Types of plants bioreactor
• Plant suspension culture.
• Chloroplast bioreactor
• Hairy root system.
• Seed based plant bioreactor.

8. Key process
Select &
design gene
high level
expression
Bio safety &
functionally
test
Purification
of desired
products
Harvesting
of plants
material
Growth of
plants in field
Selection of
transgenic
plant /cell
Plant
transformation
Regeneration
of cell

9. Production of bio molecules
BIOMOLECULE
CARBOHYDRATES PROTEIN LIPID

10. CARBOHYDRATES
• CYCLO-DEXTRIN
• Cyclodextrin are cone shaped rings formed by 6-8
subunit.
• They are hydrophilic in nature and pocket can
hydrophobic compounds
due to this property
use as therapeutic agents
Act as starch substrates.
A bacterial gene encoding cyclodextrin glycosyl
transferase.cgt

11. Fig : 1

12. PROTEIN
• Approaches
• Selection of crop species
• Choice of tissue
• Expression strategies
• Stable integration transient expression
• Production of bulk protein protein production at the desired period.
• Post translational processing
• Purification.

13. Fig : 2

14. Product obtained
Fig : 3 Tree depicting biotechnological advances using plants as bioreactors

15. Bio-plastics
• Biodegradable plastics or bio-plastics are chemically
poly hydroxyl alkanets (PHAs).
• They are currently being produced in large quantities
by microbial fermentation.
BIO-PLASTICS
PHB PRODUCTION IN PHB PRODUCTION
CYTOPLASM IN PLASTIDS

16. Fig : 5

17. PRODUCTION OF VACCINES IN PLANTS
• Vaccine a preparation introduced
into the body to simulate immunity
against a pathogen or disease.
• Production system example yeast, bacteria, fungi,
etc.

18. Table : 1

19. 2.Industrial enzyme

20. Therapeutic proteins

21. Advantages
• Low cost of production.
• Eukaryotic protein processing.
• Unlimited supply.
• No need for skilled worker.
• Safe and environmental friendly.

22. Problems
• Plant may attach different polysaccharides to protein.
• Most inserted genes are expressed at very low level in
plants.
• Some plants produce allergic compounds.

23. REFFERANCES
• U.satyanarayana biotechnology.
• Research review paper Plants as bioreactors: Recent
developments and emerging opportunities Arun K. Sharma ,⁎
Manoj K. Sharma Department of Plant Molecular Biology,
University of Delhi South Campus, New Delhi 110021, India
• www.srmuniv.ac.in/downloads.
• Transgenic plants as bioreactor Arun K. Sharma et al. Indian
journal of biotechnology (vol.3 April 2004,pp 274-290)

24. Thank You