Endothelial cells , the lining cells of blood vessels , are involved in many physiological and pathophysiological process, including haemostasis, vasoregulation, inflammation, angiogenesis, and the extravasation of fluids, macromolecules, hormones and leucocytes. This work was carried out to investigate the isolation, culture and characterization of human umbilical vein endothelial cells (HUVECs) in vitro by the help of Flow cytometry and Immunocytochemistry. Moreover, protocol was standardized to improve the cell yield and growth of these cells in culture. The endothelial cells were isolated by collagenase digestion and were seeded in tissue culture flasks using Dulbeccos modified Eagles medium (DMEM) –LG(Low Glucose) and M-200 media specific for ECGM (Endothelial Cell Growth Media ) containing LSGS( Low Serum Growth Supplement) and growth factor containing the (VEGF) and (bFGF) This study showed that HUVECS attached to to the gelatin coated tissue culture plates and become confluent after 3 days in vitro .HUVECs displayed cobblestone morphology at confluence with granular cytoplasm. HUVECs were positive for CD31- 3 % positivity, VE-Cadherin - 18.8% and vWF- 30.3%. Immunocytochemistry helped to anatomically a visualize the localization of endothelial cells marker. The cells produced from this technique grew well for extend periods and retained the normal characteristics of primary HUVECs, making them invaluable tools for researchers. Keywords: HUVECs., Flow Cytometry, Immunocytochemistry,VE-Cadherin,Vwf

1. Conference Secretariat:
Dr. Sheo Mohan Singh, Director, ICSCCB, R.H. No. 2, Ujwal Regalia,
Near Prabhavee Tech Park Building, Baner Road, Pune - 411045, India
Web: www.icscc.in, Email: icscc2015@gmail.com, Tel: +91-9545089202
6th
International Conference on Stem Cells and Cancer (ICSCC-2015):
Proliferation, Differentiation, and Apoptosis
Yashada Auditorium, Baner Road, Pune, India, 2 - 5 October, 2015
Dear Mr./Ms./Dr. Rohit Chamoli,
We are pleased to inform you that your abstract titled “Isolation of Human Umbilical Vein
Endothelial Cells (HUVECs) from Umbilical Cord (UC) and its characterization in terms of Cell
Surface Markers via Immunofluroscence & Flow Cytometry and Angiogenic Assay” (Authors:
Dr. Sujata Mohanty1
, Anupama Kakkar1
, Rinkey Sharma1
, Rajan Narayan2
, Rohit Chamoli3
, Nitul Sharma4
,
Gaurav Nigam4
) has been accepted for POSTER presentation at the “6th
International Conference on
Stem Cells and Cancer (ICSCC-2015): Proliferation, Differentiation & Apoptosis” which is being
organized from 2-5 October 2015, Pune, India.
Your presentation is scheduled on 05 October 2015. You can book your accommodation through
conference website http://www.icscc.in/accommodation/ or you can book directly on your own.
Poster size should be 3 ft width x 4 ft length.
Please register for the conference if you have not done so already. If you have already registered for
the conference then you can come directly to registration desk at 8am on 2nd
October 2015.
Please email to icscc2015@gmail.com for any queries.
We look forward to welcoming you at the 6th
ICSCC-2015, Pune, India.
Yours sincerely,
Dr. Sheo Mohan Singh, Organizer,
MSc(UK), PhD(Germany), PDF(USA,UK)
Co-organizers:
Dr. Keith Humphries, Director, Terry Fox Laboratory, BC Cancer Agency, Vancouver, Canada
Dr. Christian Buske, Director, Institute of Experimental Cancer Research, University Hospital Ulm, Ulm,
Germany
Dr. Laxman Gangwani, Associate Professor, Department of Biomedical Sciences, Texas Tech University
Health Sciences Center, El Paso, USA
Dr. Arun K. Trivedi, Senior Scientist, Biochemistry Division, Central Drug Research Institute (CDRI),
Lucknow, India