DNA Fingerprinting

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DNA Fingerprinting

  1. 1.  Your DNA fingerprint is a “fingerprint” of your DNA after it has been cut into fragments. No one has the exact same DNA Fingerprint except for identical twins. DNA Fingerprinting is used as a way to reliably identify an individual. The chance that two people would have the same DNA Fingerprint is nearly impossible
  2. 2. After a DNA sample is found, it is placed in a test tube withseveral chemicals that will help break the cells down to releasethe DNA.
  3. 3. Next, a UV spectrophotometer is used to see how muchDNA has been extracted. Light rays are passed through the sample of DNA. The amount of light that is absorbed depends on how much DNA is in the sample. The more DNA there is, the more light is absorbed.
  4. 4.  Then, the polymerase chain reaction is used to increase the amount of DNA available
  5. 5. After more DNA has been created, restrictionenzymes are used to cut the DNA at specific places.
  6. 6. The DNA pieces are then sorted using a technique calledelectrophoresis. This uses an electrical current to sort the fragments by size. When charged, smaller fragments of DNA will move farther than larger pieces.
  7. 7. The DNA is then transferred to a nylon sheet and probesare added so the DNA fingerprint can be seen.DNA Fingerprinting Video
  8. 8.  DNA fingerprinting is often used as biological evidence in criminal cases.  If DNA of a suspect matches DNA found at a crime scene, then the DNA fingerprint can be used as evidence to prove someone guilty or innocent. DNA fingerprinting can also be used in paternity testing.  If the DNA fingerprint of a child is similar to that of a potential parent, then they are most likely related. DNA fingerprinting is used to diagnose inherited disorders in both prenatal and newborn babies. In the U.S. military, DNA fingerprinting is starting to be used as a way to identify casualties or people that are missing in action. It can also help people that were proven guilty, but were innocent, be proven innocent and taken out of jail.
  9. 9. ╪ When used in court cases, evidence from DNA fingerprinting is not always accepted. ╪ Not everyone is going to understand what they are being showed.╪ Test can be inaccurate and in some cases, matches can be wrong.╪ There are DNA databanks full of DNA from convicted criminals and suspects. These samples can be used to help solve cases, but not everyone agrees that DNA should be kept on record.

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