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Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
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Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique

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Journal Club 3 : Nitric Oxide Sensors

Journal Club 3 : Nitric Oxide Sensors

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  • การหาระยะเวลาของการดูดซับ Nitric Oxide โดยหารให้ผ่านทางลมหายใจ โดยใช้วิธี non-invasive แบบ Image technique
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    • 1. Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique Karel J. Zuzak ,at all ( Circulation. 2001;104:2905-2910.)
    • 2. Nitric Oxide (NO) Endothelium-derived relaxing factor (EDRF)
    • 3. NADPH O 2 + Production of NO
    • 4. Effector mechanisms of NO Ca 2+ NO-dependent Vasodilators -Acetylcholine -Bradykinin Blood Flow Shearing Forces R Endothelial Cell Smooth Muscle Cell NOS GC cGMP Lumen Ca 2+ K +
    • 5. Cardiovascular disease Diabetes mellitus Type I,II Cardiovascular disease Endothelial dysfunction
    • 6.  
    • 7.  
    • 8.  
    • 9.  
    • 10. Methods
      • Noninvasively
      • Real time
      • in vivo
    • 11. Spectroscopic Determination of Percentage of HbO2 541 576 555
    • 12. Imaging system.
      • Liquid crystal tunable filter (LCTF)
      • Charge-coupled device detector (CCD)
      • Analog-to-digital converter (ADC)
    • 13. Instrumentation
      • A quartz tungsten halogen light source
      • (Oriel Instruments)
      • A mirror ,Liquid crystal tunable filter(LCTF)
      • (Cambridge Research and Instrumentation)
      • Lens (Nikon)
      • CCD detector (Roper Scientific)
      • GX1P computer (Dell)
    • 14. Spectroscopic Determination of Percentage of HbO 2
      • Reference spectra of a 100% HbO 2 and deoxy-Hb
        • Standard methods using blood collected from a healthy, nonsmoking
        • 7 mL of hemolysates was mixed with 30 mg of sodium dithionite
        • The HbO 2 spectrum displays distinct peaks at 541 and 576 nm
        • Deoxy-Hb displays a single peak at 555 nm
    • 15. Study Population
      • 9 nonsmoking healthy (5 men and 4 women)
      • Average age of 32±4 years
      • Clinical history, physical examination
      • ECG, and routine laboratory analyses
      • No evidence of cardiovascular disease
    • 16. Protocol
      • refrained drinking alcohol and beverages containing caffeine for ≥12 hours
    • 17. Protocol 1. breathed room air Tem~22°C 5% dextrose in water (D5W) 1 mL/min NG-monomethyl-L-arginine (L-NMMA) 4 to 8 mol/min 20 min 2. NO 80 ppm blood flow was measured by venous occlusion plethysmography(Hokanson) NOS inhibitor during which time hyperspectral images were collected
    • 18. Statistical Analysis
      • Differences between population means were compared by a 2-tailed
    • 19. Results
    • 20.  
    • 21.  
    • 22. Results
    • 23. Results
    • 24. Discussion
      • Noninvasive techniques for providing rapid, repetitive assessment of tissue perfusion could be of considerable utility in the management of a variety of vascular diseases. In addition to assessing and quantifying the concentration of a specific chemical species, such as HbO 2 and deoxy-Hb,
    • 25. Discussion
      • hyperspectral imaging provides representations of the spatial distribution of a molecular species at a given time or over time.
      • Accordingly, acquisition of a time-resolved profile representing the spatial dynamics of physiologically important molecules indicative of tissue perfusion could prove useful in assessing the efficacy of a given therapeutic intervention on vascular disease.
    • 26. Thank You

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