Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique
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Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique

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Journal Club 3 : Nitric Oxide Sensors

Journal Club 3 : Nitric Oxide Sensors

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  • การหาระยะเวลาของการดูดซับ Nitric Oxide โดยหารให้ผ่านทางลมหายใจ โดยใช้วิธี non-invasive แบบ Image technique

Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique Presentation Transcript

  • Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique Karel J. Zuzak ,at all ( Circulation. 2001;104:2905-2910.)
  • Nitric Oxide (NO) Endothelium-derived relaxing factor (EDRF)
  • NADPH O 2 + Production of NO
  • Effector mechanisms of NO Ca 2+ NO-dependent Vasodilators -Acetylcholine -Bradykinin Blood Flow Shearing Forces R Endothelial Cell Smooth Muscle Cell NOS GC cGMP Lumen Ca 2+ K +
  • Cardiovascular disease Diabetes mellitus Type I,II Cardiovascular disease Endothelial dysfunction
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  • Methods
    • Noninvasively
    • Real time
    • in vivo
  • Spectroscopic Determination of Percentage of HbO2 541 576 555
  • Imaging system.
    • Liquid crystal tunable filter (LCTF)
    • Charge-coupled device detector (CCD)
    • Analog-to-digital converter (ADC)
  • Instrumentation
    • A quartz tungsten halogen light source
    • (Oriel Instruments)
    • A mirror ,Liquid crystal tunable filter(LCTF)
    • (Cambridge Research and Instrumentation)
    • Lens (Nikon)
    • CCD detector (Roper Scientific)
    • GX1P computer (Dell)
  • Spectroscopic Determination of Percentage of HbO 2
    • Reference spectra of a 100% HbO 2 and deoxy-Hb
      • Standard methods using blood collected from a healthy, nonsmoking
      • 7 mL of hemolysates was mixed with 30 mg of sodium dithionite
      • The HbO 2 spectrum displays distinct peaks at 541 and 576 nm
      • Deoxy-Hb displays a single peak at 555 nm
  • Study Population
    • 9 nonsmoking healthy (5 men and 4 women)
    • Average age of 32±4 years
    • Clinical history, physical examination
    • ECG, and routine laboratory analyses
    • No evidence of cardiovascular disease
  • Protocol
    • refrained drinking alcohol and beverages containing caffeine for ≥12 hours
  • Protocol 1. breathed room air Tem~22°C 5% dextrose in water (D5W) 1 mL/min NG-monomethyl-L-arginine (L-NMMA) 4 to 8 mol/min 20 min 2. NO 80 ppm blood flow was measured by venous occlusion plethysmography(Hokanson) NOS inhibitor during which time hyperspectral images were collected
  • Statistical Analysis
    • Differences between population means were compared by a 2-tailed
  • Results
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  • Results
  • Results
  • Discussion
    • Noninvasive techniques for providing rapid, repetitive assessment of tissue perfusion could be of considerable utility in the management of a variety of vascular diseases. In addition to assessing and quantifying the concentration of a specific chemical species, such as HbO 2 and deoxy-Hb,
  • Discussion
    • hyperspectral imaging provides representations of the spatial distribution of a molecular species at a given time or over time.
    • Accordingly, acquisition of a time-resolved profile representing the spatial dynamics of physiologically important molecules indicative of tissue perfusion could prove useful in assessing the efficacy of a given therapeutic intervention on vascular disease.
  • Thank You