Here is the presentation about my final year project. I, intentionally, did not put every information on the slide because I decided to mention it on my presentation speech. That's one of my way to keep the audience with me.

1. Sandra Cory Clarisa Tarigan and Dwina Roosmini
Department of Environmental Engineering
Faculty of Civil and Environmental Engineering Institut Teknologi Bandung
u

2. “You might have
unintentionally
quench your thirst on
water that contains
heavy metal
substances”
6+
Cr
other
heavy
metal
s..

3. Raw water for drinking
water in
Bandung, Purwakarta,
Karawang, Bekasi and
Jakarta comes from
Citarum River.

4. In some experiments
conducted to examine the
water quality of Citarum river
still can be found toxicant that
is at alarming level.

5. One of the industries that
discharge their waste water
into the Citarum River is the
electroplating industry.

6. Preliminary study of acute
toxicity test using flow-through
test method with daphnia magna
as test animal on an
electroplating industry’s wwtp
effluent

7. What’s inside?

8. - Methodology
- Results
- Conclusion
- Introduction

9. 2
3
4
Introduction
1
Indonesia does not have any
standards governing the toxicity of
waste water parameters. In some
countries, a feasibility test for the
industrial wastewater effluent
based on the level of toxicity has
been developed.

10. 1
3
4
2
PRELIMINARY
- Field Survey
- Literature Study
Daphnia magna cultures Retrieving samples
Acute Toxicity Test
(WET)
- Flow-through test
- LC50-96 hours
- Measure:
pH, DO, temperature, alk
alinity, hardness, salinity,
ammonia
WWTP Effluent & Influent
Characteristic Test
Parameters:
TSS, COD, pH, CN, Cr, Cu, P
b, Ni, Cd, Zn
Analysis and discuccion
Conclusion and feedback
methodology

11. 1
3
4
2 Acute toxicity test
Both the range-finding & definitive tests were done by exposing the
test organism to 5 various sample concentrations.
The value of LC50 is obtained from PROBIT
- Range-finding test
- Definitive test
- LC50
Using flow-through test method
Using static test method
6,25%, 12,5%, 25%, 50%, 100%

12. 1
3
4
2 DAPHNIA ma
The early stage of
Daphnia magna using is
the process of
culturing test animals
Daphnia magna
that is used are
those who aged <
24 hours and will
not be fed during
the test

13. 1
3
4
c
2 Culturing DAPHNIA m
“The full grown
Daphnia magna
took just a few days
to sprout. Thus, it
does not take a
long time to prepare
for the test
organism culture.”
-Womsiwor

14. 1
3
4
2 Flow-through test React
- Made of acrylic (64 x 74 x 40 cm)
- The effluent flow is pumped continually
to the reactor
a

15. 1
3
4
2
50 ml
250 ml
2000 ml
11 cm
15 cm
12,5 cm
40 cm
2.5 cm
wood
1 2 3
4 5
60+4cm
70 + 4 cm
AQUA
Control
Flow-through test React

16. 1
2
4
3
results
CHARACTERISTIC
WASTE WATER

17. 1
2
4
3
results
CHARACTERISTIC
WASTE WATER
Baku mutu =
0.2 mg/l
Baku mutu =
0,1 mg/l

18. CHARACTERISTIC
WASTE WATER
1
2
4
3
results
Parameters Standard Did not meet
standard (%)
Highest Removal Eff.
(%)
Average Removal
Eff. (%)
TSS 20 mg/l 53.33 94.4 39.77
pH 6-9 66.67 - -
COD 100 mg/l 80 69.23 -3.97
CN 0.2 mg/l 0 56.04 -40.34
Cr 0.5 mg/l 100 36.34 -6.72
Cu 0.6 mg/l 0 98.26 86.11
Cd 0.05 mg/l 0 87.5 -20
Ni 1 mg/l 0 - 0
Zn 1 mg/l 100 84.79 49.29
Pb 0.1 mg/l 0 83.33 28.57

19. 1
2
4
3
results
Acute
toxicity
test
Range-finding test
Definitive test
LC50 17.096 %
12.5-25%
(temporary
LC50)
15%, 18%, 20%, 21%, 25
%
(5 new various
concentrations of
effluent)

20. 1
2
4
3
results
Observation Dilution pH DO (mg/l) Temp (oC) Conductivity (mS)
24 hr 12,5 % 8,93 4,18* 26,67 0,84
15 % 8,65 5,38 26,3 0,46
18 % 8,9 5,65 27 0,78
21 % 8,7 6,07 26,3* 1,13
25 % 8,87 4,96 26,67 1,03
0 % (control) 8,27 5,30 27 0,25
48 hr 12,5 % 8,5 5,54 26,8 1,11
15 % 8,3 5,35 27 1,36
18 % 8,46 4,80 27 0,76
21 % 8,57 6,33 28,5 1,65
25 % 8,33 5,11 27,3 1,25
0 % (control) 8,6 4,88 28 0,46
72 hr 12,5 % 8,6 4,70 27 1,11
15 % 8,87 6,16 27 0,41
18 % 8,5 4,48 27 0,59
21 % 8,9 5,79 28 0,88
25 % 9,17* 5,56 28.3 1,45
0 % (control) 8,5 5,81 29* 0,33*
96 hr 12,5 % 8,25* 6,06 28 0,72
15 % 8,3 5,28 28 1,21
18 % 8,75 5,50 27,7 1,39
21 % 8,9 6,67* 27 0,43
25 % 8,8 4,58 28,5 1,01
0 % (control) 8,6 5,60 28 1,72*
Acute
toxicity
test

21. 1
2
4
3
results
Acute
toxicity
test
Value pH DO
(mg/l)
Temp
(oC)
Conductivity
(mS)
Highest 9.17 6.67 29 1.72
Lowest 8.25 4.18 26.3 0.33
Water Quality On Chamber Test During Definitive Test
1. pH
It is written that optimum pH for living place of Daphnid is between 8,5-9,5. The
solution test is base and still in optimum range.
2. DO
Daphnid can live well in water that contains dissolved oxygen from 4-6 mg/l. The
DO contained in solution test is still in optimum range. This is one of the benefit
of using flow-through test method. By flow-through test method, DO
concentration in reactor is more preserved.

22. 1
2
4
3
results
Acute
toxicity
test
Value pH DO
(mg/l)
Temp
(oC)
Conductivity
(mS)
Highest 9.17 6.67 29 1.72
Lowest 8.25 4.18 26.3 0.33
Water Quality On Chamber Test During Definitive Test
3. Temperature
Daphnid can live well on temperature range from 18 to 26oC. The temperature
that is occurred goes beyond the optimum temperature range. But, the daphnids
has been cultured in the location for about 4 weeks. This gives them time to adapt
with the temperature on the location.
4. Conductivity
Optimum conductivity value of daphnid living place is 0,5 mS. The value of
conductivity in the chamber test exceeds the optimum value. This might
contributes to the immobility of daphnid during toxicity test.

23. Value Hardness
(mg/l
CaCO3)
Salinity
(o/oo)
TSS
Influent
(mg/l)
TSS
Effluent
(mg/l)
Alkalinity
(mg/l
CaCO3)
Ammonia
(mg/l)
Highest 176,04 2,10 22,33 25,33 545 0,96
Lowest 138,18 0,80 15,33 15,33 390 -0,04
1
2
4
3
results
Acute
toxicity
test
Characteristic of Waste Water During Definitive Test
1. Hardness
The higher the value of water hardness, the more toxic its effluent. For this
parameter, there has been no established standard so that can not be concluded
whether the obtained value is categorized as high value or not.
2. Salinity
The higher the value of salinity, the higher the possibility of immobility occurred to
daphnid. But from the measurement result, it can be seen that the value of salinity
is small and doesn’t need to be worried about.

24. Value Hardness
(mg/l
CaCO3)
Salinity
(o/oo)
TSS
Influent
(mg/l)
TSS
Effluent
(mg/l)
Alkalinity
(mg/l
CaCO3)
Ammonia
(mg/l)
Highest 176,04 2,10 22,33 25,33 545 0,96
Lowest 138,18 0,80 15,33 15,33 390 -0,04
1
2
4
3
results
Acute
toxicity
test
3. TSS
The established standard of TSS is 20 mg/l. There are some samples that exceeds
the standard. Also, there are samples where TSS effluent is higher than TSS
influent.
4. Alkalinity
pH value tends to increase as the value of alkalinity increases. From the obtained
data, the data appears to be parallel with the theory.
Characteristic of Waste Water During Definitive Test

25. 1
2
3
4 After analysing the characteristics and
comparison with the effluent water quality
standards from Ministry of Environment
Decision No. 51 year 1995 on wastewater
quality standards for Metal Coatings
Industrial Activities, there are some
physical-chemical parameters that still
exceeded these quality standard. These
parameters are pH, TSS, COD, Cr and Zn. The
acute toxicity test using flow-through test
reactor and Daphnia magna as animal testing
conclusion

26. 1
2
3
4 After analysing the characteristics and
comparison with the effluent water quality
standards from Ministry of Environment
Decision No. 51 year 1995 on wastewater
quality standards for Metal Coatings
Industrial Activities, there are some
physical-chemical parameters that still
exceeded these quality standard. These
parameters are pH, TSS, COD, Cr and Zn. The
acute toxicity test using flow-through test
reactor and Daphnia magna as animal testing
conclusion

27. This research is supported and sponsored b

28. 6“Thank you for listening”
z
z

29. Preliminary study of acute
toxicity test using flow-through
test method with daphnia magna
as test animal on an
electroplating industry’s wwtp
effluent