Your SlideShare is downloading. ×
Fred Martin Micrograph Portfolio
Upcoming SlideShare
Loading in...5
×

Thanks for flagging this SlideShare!

Oops! An error has occurred.

×
Saving this for later? Get the SlideShare app to save on your phone or tablet. Read anywhere, anytime – even offline.
Text the download link to your phone
Standard text messaging rates apply

Fred Martin Micrograph Portfolio

309

Published on

Sample of images taken for MMP program using Zeiss Axioimager, Nikon, Olympus AX41, Leica

Sample of images taken for MMP program using Zeiss Axioimager, Nikon, Olympus AX41, Leica

Published in: Education, Business, Technology
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total Views
309
On Slideshare
0
From Embeds
0
Number of Embeds
0
Actions
Shares
0
Downloads
0
Comments
0
Likes
0
Embeds 0
No embeds

Report content
Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
No notes for slide

Transcript

  • 1. PROJECT PORTFOLIO FRED MARTIN 12/08/2009 Dr. Giorgi BIOSC 2
  • 2. Invt4_10x_CompositeRGB_002.jpg 20091207 /Nikon2 /10x ; DIC: 73ms/ 1,503/ 0.95/ 4,095 R: 97ms/ 79/ 0.63/ 4,095 G: 200ms/ 239/ 0.95/4,095 B:500ms/223/0.77/4,095 SPECIMEN (A1) NIKON, FLUORESENCE, MOLECULAR PROBES SLIDE: TISSUE
  • 3. invt4_a1_Composite_RG_DapiLUT_75.jpg 20091123 /Nikon2 /20x ; DIC: 20ms/ 79/ 0.94/ 3,310 R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830 SPECIMEN (A2) NIKON, FLUORESENCE, MOLECULAR PROBES SLIDE: TISSUE
  • 4. FluoCells prepared slide #2: BPAE bovine pulmonary artery endothelial cells Image A - 20091105: Zeiss #1 20x ZEISS, FLUORESENCE, MOLECULAR PROBES SLIDE: CELLS
  • 5. FluoCells ® prepared slide #1: BPAEC, Zeiss 63x 20091016_INVG1_AREA3_CompositeAdj_jpg.jpg ZEISS, FLUORESENCE, MOLECULAR PROBES SLIDE: CELLS
  • 6. NIKON, FLUORESENCE, MOLECULAR PROBES SLIDE: CELLS FM_INVT1_A1_CompositeAdjRGB_xDIC BPAE CELLS INVITROGEN #1
  • 7. 20x_LilyAnther_Fluro_RGB.jpg 20091123 /Nikon2 /20x ; R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830 SPECIMEN (B) NIKON, FLUORESENCE
  • 8. FM_lycopodiumCaptured_FluoroRGBnDIC_20x_2.jpg 20091123 /Nikon2 /20x ; DIC: 20ms/ 79/ 0.94/ 3,310 R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830 SPECIMEN (C) NIKON, FLUORESENCE + DIC
  • 9. SPECIMEN (F) Typha_10x_Composite_001_RGB_Adj 20091207 /Nikon2 /10x ; DIC: 73ms/ 1,503/ 0.95/ 4,095 R: 97ms/ 79/ 0.63/ 4,095 G: 200ms/ 239/ 0.95/4,095 B:500ms/223/0.77/4,095 COMPOSITE CHANNEL: RED CHANNEL: GREEN CHANNEL: BLUE NIKON, FLUORESCENSE (3 CHANNELS + OVERLAY)
  • 10. Pollen Grain: Abutilon x hybridium @ 20x
    • EM Channel: Green Exp: 96.7ms Gain:7.1 Offset: -876
    EM Channel: Red Exp: 96.7ms Gain:7.1 Offset: -876 EM Channel: (BF) Exp: 16.7ms Gain: 4.5 Offset: -161 Composite OLYMPUS, OWN SPECIMEN, FLUORO OVERLAY + 3 CHANNELS
  • 11. fm__pseudocolor_lycopod_20x_CompositeRGBnDIC.jpg SPECIMEN (D) 20091207 /Nikon2 /20x ; “R”: 13ms/ 159/ 0.51/ 2,830 “G”: 6.6ms/ 159/ 0.51/ 2,830 “B”: 12.0ms/ 159/ 0.51/ 2,830 NIKON, BRIGHTFIELD (3 CHANNEL PSEUDO-COLORIZATION)
  • 12. Multichannel _Shrimp_Pseudocolor2_RGB.jpg SPECIMEN (E) 20091118 /Nikon4 /20x ; R: --ms/ --/ --/ -- G: --ms/ --/ --/ -- --B: ms/ --/ --/ -- Note: metadata not logged NIKON, BRIGHTFIELD (3 CHANNEL PSEUDO-COLORIZATION)
  • 13. Cilia PSCCE Cells Basal Lamina Pseudo Stratified Ciliated Columnar Epithelial Cross section: area 3 image Cilia Basal Lamina Nucleus Cilia (Apparent Stratification) Exp. 500.7ms Hist: 79/252 Gamma: 0.99 Auto_exp: OFF Gain: 3.1x 2009.10.01 Leica #2 60x (dry) Nucleolus { } LEICA, BRIGHTFIELD
  • 14. PSCCE Cells Cell Membrane Cilia (Apparent Stratification) Nuclei Basal Lamina LEICA, BRIGHTFIELD 48 BitDepth: 20 / 255 Histogram: On Auto_Exp? 0.97 Gamma: 2009-10-07 DateImage: 53 % Brightness: 2.2 x Gain: 1.0 s Exposure:
  • 15. ZEISS, DIC FM_INVT4_A1_DIC-0005.JPG 20091209 /ZEISS 1 /20x ; DIC : EXP 69ms/ CT -0.50 / GAMMA 1.0 / BRT 1.0
  • 16.
    • Interphase
    • Metaphase
    • Anaphase
    • Telophase
    • Prophase
    RESULTS: DIGITAL COUNT DETAIL – FIELD OF VIEW A: Onion Root Tip Meta Data Scope: Olympus 3 (A) 10x, rheo: full Iris ~ 30% 1.1 ms 2.8 gain -1,860 offset Note: Manual count done at 20x on Olympus3 OLYMPUS, MORPHOMETRY

×