Nikon Motorized Microscope
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The document summarizes specimens and imaging settings used to capture microscope images for a project. Specimen A was a mouse intestine section stained with fluorescent dyes. Specimen B was a lily anther cross section. Specimen C was a longitudinal section of lycopodium strobilus. Exposure times and settings are provided for brightfield and fluorescent images of each specimen. Additional specimens included brine shrimp, more lycopodium sections, and a Typha latifolia section.
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Nikon Motorized Microscope
- 1. PROJECT Save An Hour - SAH FRED MARTIN 12/02/2009 Dr. Giorgi BIOSC 2
- 2. Information on Specimens Used: General: All slides were prepared slides taken from the MMP slide library Fluorescent channels on Lycopodium BF and Fluoro images were acquired manually due to NIS exposure changing issue in auto mode. Lily Anther and Brine Shrimp were acquired in auto-mode. All post image processing done in ImageJ MetaData Format: Date/Scope/Objective; (Channel): Exposure/ BlackLevel/ Gamma/ WhiteLevel Specific: Used For Fluorescent Images Specimen A1: Invitrogen Slide #4 16 µm cryostat section of mouse intestine w/ DIC: stained with Alexa Fluor 350 (blue) wheat germ agglutinin to stain the mucus of goblet cells . The filamentous actin prevalent in the brush border was stained with red-orange–fluorescent Alexa Fluor 568 phalloidin (A-12380) and SYTOX Green nucleic acid stain (S-7020) Specimen B: Lily Anther Cross Section with Pollen (Carolina Slide # nnnn) Specimen C1: Lycopodium Mature Strobillus (longitudinal section) (Slide # nnnn) Used For Brightfield (Pseudo colorization) Images: Specimen D: Brine Shrimp (Artemia Nauplii) Whole Section mount (Olympus Sample Slide # nnnn) Specimen E: Lycopodium Mature Strobillus (longitudinal section) (Slide # nnnn) Used For Extra Credit/Fluorescent/DIC Overlay + DIC only Images: Specimen F: Typha Latifolia (ls) slide # nnnn Specimen A2: Invitrogen Slide #4 16 µm cryostat section of mouse intestine w/ DIC: area 2 Specimen C2: Lycopodium Mature Strobillus (longitudinal section)
- 3. Invt4_10x_CompositeRGB_002.jpg 20091207 /Nikon2 /10x ; DIC: 73ms/ 1,503/ 0.95/ 4,095 R: 97ms/ 79/ 0.63/ 4,095 G: 200ms/ 239/ 0.95/4,095 B:500ms/223/0.77/4,095 SPECIMEN (A1)
- 4. 20x_LilyAnther_Fluro_RGB.jpg 20091123 /Nikon2 /20x ; R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830 SPECIMEN (B)
- 5. FM_lycopodiumCaptured_FluoroRGBnDIC_20x_2.jpg 20091123 /Nikon2 /20x ; DIC: 20ms/ 79/ 0.94/ 3,310 R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830 SPECIMEN (C)
- 6. fm__pseudocolor_lycopod_20x_CompositeRGBnDIC.jpg SPECIMEN (D) 20091207 /Nikon2 /20x ; “R”: 13ms/ 159/ 0.51/ 2,830 “G”: 6.6ms/ 159/ 0.51/ 2,830 “B”: 12.0ms/ 159/ 0.51/ 2,830
- 7. Multichannel _Shrimp_Pseudocolor2_RGB.jpg SPECIMEN (E) 20091118 /Nikon4 /20x ; R: --ms/ --/ --/ -- G: --ms/ --/ --/ -- --B: ms/ --/ --/ -- Note: metadata not logged
- 9. SPECIMEN (F) Typha_10x_Composite_001_RGB_Adj 20091207 /Nikon2 /10x ; DIC: 73ms/ 1,503/ 0.95/ 4,095 R: 97ms/ 79/ 0.63/ 4,095 G: 200ms/ 239/ 0.95/4,095 B:500ms/223/0.77/4,095 COMPOSITE CHANNEL: RED CHANNEL: GREEN CHANNEL: BLUE
- 10. invt4_a1_Composite_RG_DapiLUT_75.jpg 20091123 /Nikon2 /20x ; DIC: 20ms/ 79/ 0.94/ 3,310 R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830 SPECIMEN (A2)
- 11. SPECIMEN (C2) FM_lycopodiumCaptured DIC 10ms20x.JPG 20091207 / Nikon2 / 20x ; DIC: 40ms/ 79 / 0.94 / 3,310