Yeast Two Hybrid system uses a reporter gene to detect the interaction of pair of proteins inside the yeast cell nucleus. In the yeast Two Hybrid System, The interaction of target protein to the protein will bring together transcriptional activator, which then switches on the expression of reporter gene.

1. Yeast Two-Hybrid System
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2. What is Two Hybrid System
• Two Hybrid system is for Isolating and
Studying Protein-Protein Interactions.
• This system uses a reporter gene for detecting
the interaction of pair of proteins in yeast cell
nucleus.
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3. What are the Uses / Applications of
Two Hybrid System
• Two-hybrid screens are low-tech; they can be
carried out in any lab without sophisticated
equipment.
• Two-hybrid screens can provide an important first
hint for the identification of interaction partners.
• The assay is scalable, which makes it possible to
screen for interactions among many proteins.
• Yeast two-hybrid data can be of similar quality to
data generated by the alternative approach
of coaffinity purification followed by mass
spectrometry (AP/MS).
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4. How Two Hybrid System Works
• The DNA sequence that encodes target
protein, is fused with the DNA that encodes
the DNA binding domain of gene activator
protein using recombinant DNA technology.
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5. Yeast Two Hybrid System
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6. Conclusion
• Bait Protein - protein fused to a DNA-binding
domain localizes it to the regulatory region of
a reporter gene.
• Prey Protein - Specially designed protein in the
cell nucleus.
• Interaction of Bait and Prey bring about the
activation of reporter gene.
• The expression of reporter gene helps the
yeast cells survive on selective medium.
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7. Thank You
• Bio-Resource
• http://technologyinscience.blogspot.com/201
4/07/yeast-two-hybrid-system-for-
protein.html
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