The document summarizes research on the dechlorination of polychlorinated biphenyl (PCB) congeners by the anaerobic bacterium Dehalococcoides. Key findings include:
1) Dehalococcoides uses PCBs as a terminal electron acceptor and can effectively dechlorinate various PCB mixtures.
2) Experiments showed some PCB congeners (234 CB and 2345 CB) underwent more dechlorination than others (245 CB and 235 CB).
3) Further research is needed to understand congener specificity and identify reductive dehalogenase enzymes responsible for dechlorinating different PCBs.
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Rotation I Presentation
1. Dechlorination of Polychlorinated Biphenyl (PCB) Congeners by Dehalococcoides By: David VanHoute First Year Graduate Student Rensselaer Polytechnic Institute 11/12/2010
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Editor's Notes
Stress need for terminal electron acceptor; specific Rdase enzymes, N and P process generally preferred.
36 in others
Anoxic reagents, anaerobic chamber, crimp and purge vials, limit oxygen We know that Dehalococcoides dechlorinates mixtures of PCBs, but don’t know which congeners.
Retention times show how long specific congeners remain on GC column. Shows where product is seen and substrate
MV provides ample supply of H, no need for hydrogenase activity by microbes.
Both 234 and 2345 are doubly flanked congeners, significant product with removal of meta chlorine.
234 and 2345 are doubly flanked chlorines that get removed, don’t really know why.
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