1. PCR AMPLIFICATION, CLONING, SEQUENCE DETERMINATION, AND BIOINFORMATICS ANALYSES OF NOVEL PLANT GAPDH GENES FROM CYPERUS ALTERNIFOLIUS , SCHEFFLERA ACTINOPHYLLA AND TROPICAL FLORA ENDEMIC TO PUERTO RICO Lydia E. Cortes and Dr. Michael Rubin Department of Biology and RISE Program University of Puerto Rico at Cayey

9. CONTINUATION OF TRANSFORMATION Amp plates- 900 and 50 ul Overnight (both) LB Broth + Amp solution (100 ul of Amp and 100 ml of LB Broth)

10. PLASMID PURIFICATION Resuspend in 200ul resuspension solution 250 ul lysis solution 250ul neutralization solution

11. CONTINUATION PLASMID PURIFICATION Move to tube With column Add 200 ul matrix 2 minutes 500 ul Wash solution 100 ul water 1 minute

12. SEQUENCE DETERMINATION

13. CONTINUATION OF SEQUENCE DETERMINATION 10 ul of DNA + 1 ul of primer Pipet 10 ul Seal and mail

16. PRELIMINARY RESULTS: CLONING GEL No DNA pGAP Arabidopsis Marker Cyperus Marker Schefflera

17. PRELIMINARY RESULTS OF TRANSFORMATION Type of DNA Number of cells in 50 microliters Number of cells in 900 microliters CYPERUS ALTERNIFOLIUS 17 440 SCHEFFLERA ACTINOPHYLLA 10 560

19. PCR AMPLIFICATION, CLONING, SEQUENCE DETERMINATION, AND BIOINFORMATICS ANALYSES OF NOVEL PLANT GAPDH GENES FROM CYPERUS ALTERNIFOLIUS , SCHEFFLERA ACTINOPHYLLA AND TROPICAL FLORA ENDEMIC TO PUERTO RICO Lydia E. Cortes and Dr. Michael Rubin Department of Biology and RISE Program University of Puerto Rico at Cayey