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GRM 2013: Improving phosphorus efficiency in sorghum by the identification and validation of sorghum homologs for Pup1, a major QTL underlying phosphorus iptake in rice (SorghumPup1) -- J Magalhaes (redacted)
 

GRM 2013: Improving phosphorus efficiency in sorghum by the identification and validation of sorghum homologs for Pup1, a major QTL underlying phosphorus iptake in rice (SorghumPup1) -- J Magalhaes (redacted)

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These slides are a redacted (by the presenter) version of what was presented at the meeting

These slides are a redacted (by the presenter) version of what was presented at the meeting

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    GRM 2013: Improving phosphorus efficiency in sorghum by the identification and validation of sorghum homologs for Pup1, a major QTL underlying phosphorus iptake in rice (SorghumPup1) -- J Magalhaes (redacted) GRM 2013: Improving phosphorus efficiency in sorghum by the identification and validation of sorghum homologs for Pup1, a major QTL underlying phosphorus iptake in rice (SorghumPup1) -- J Magalhaes (redacted) Presentation Transcript

    • Improving Phosphorus Efficiency in Sorghum by the Identification and Validation of Sorghum Homologs for Pup1, a Major QTL Underlying Phosphorus Uptake in Rice (SorghumPup1) ICRISAT - Mali Eva Weltzien, Fred Rattunde, Willmar Leiser IRRI/JIRCAS Sigrid Heuer (IRRI) Rico Gamuyao (IRRI) Matthias Wissuwa (JIRCAS) GCP – GRM 2013, Lisbon - Portugal Moi Unversity Sam Gudu INRAN - Niger Soumana Souley Cornell University/USDA-ARS Leon Kochian (USDA/CU) Randy T Clark (USDA/CU) Brittany Green (USDA/CU) Jon Shaff (USDA/CU) Sharon Mitchell (IGD/CU) Sylvia de Sousa Robert Schaffert Claudia Guimarães Lidiane Braga (PhD S) Gabriel Corradi (PhD S) Barbara Negri (Und) Fabiano Matos (Und) ESALQ/USP Antonio Augusto F. Garcia Rodrigo Gazaffi ICRISAT - Niamey Bettina Haussmann and Tom Hash Jurandir Magalhaes Barbara H Maciel (PhD student)
    • A Molecular Tool Kit for Highly Weathered Tropical Soils Al toxicity High P fixation Low activity clay Variable charge > 70 % Kaolinate/ Fe and Al oxidesEquator Capricorn Low P soils Acid soils (Al tox) NH COOH Cytoplasm 3 * AltSB Pstol1 Plant Phys 156-1041
    • Fe-O Citrate PO4 -- Band A Band B Band C 10 ppm (P) 5 ppm (P) 0 ppm (P) 10 ppm (P) 5 ppm (P) 0 ppm (P) 0 - 20 cm - Spatial models (r/c) - Environmental covariates - Phenological covariates Phenotyping for Phosphorus Efficiency - Soil moisture strongly impaires P diffusion - PAE x PUTIL
    • Phenotyping for Root Morphology (2D) - Importance for PAE - Shallow roots?
    • PSTOL1 increases grain yield PSTOL1 increases early root growth
    • Comparative Strategy to Validate Pstol1 Homologs as Genes Conferring Enhanced P acquisiton Sorghum: association mapping US (converted) Panel Contrasting parental linesPstol1 - Rice Maize: QTL mapping L22 Grain yield: low P soils Root morphology: nutrient solution Wide Diversity Panel Parentoni et al. (2010) Maydica
    • Polymorphism Discovery for Linkage and Association Mapping • Primer design for amplicon sequencing • SNP detection • Conversion to the Kaspar system • Genotyping in the association panel Primer-BLAST Conserved Regions Paralog-specific primers
    • Step 2: Producing F1: Ms3ms3 Step 3: Self-pollinate F1s Step 4: Random Matting Cycle 1 Step 5: Random Matting Cycle 2 Step 1: Base population development Step 6: - Random Matting Cycle 3 - Pup1/AltSB validation Selection for AltSB based on allele mining with tag markers Genotyping Genotyping S1s to produce S2s Phenotype S2s and relate genotypic information on AltSB/Pstol1 with the phenotype for validation - Provides means to validate Pup1/AltSB (higher LD) - Effect estimate - Amenable to gene pyramidation - Contingency plan based on a familiar breeding scheme (high risk project) - Entry points for new material (SorghumCI) - Cultivar release, regardless Sorghum RMPs: pyramiding AltSB and multiple Pstol1 homologs 4 cycles of random matting Products: - Agronomic performance under low P (SAP) - Root morphology and architecture (SAP) - Root morphology QTL - Functional markers