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Which of the following are NOT assumptions made in the derivation of the Michaelis-
Menten equation for a one-substrate, one-product reaction? 1.) [S] >> [Etotal]. 2.) The
rate of formation of ES equals its rate of breakdown.3.) The rate of breakdown of ES to
E + P is much faster than the rate of breakdown of ES back to E + S. 4.) The velocity of
the catalyzed reaction is equal to k[E][S]. 5,) The concentration of ES is small relative to
the concentration of Etotal. 6.) [Etotal] >> [Efree]. 7.) The rate of reaction is equal to
k[ES]. 8.) KM = [E][S]/[ES]

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Which of the following are NOT assumptions made in the derivation of t.pdf

  • 1. Which of the following are NOT assumptions made in the derivation of the Michaelis- Menten equation for a one-substrate, one-product reaction? 1.) [S] >> [Etotal]. 2.) The rate of formation of ES equals its rate of breakdown.3.) The rate of breakdown of ES to E + P is much faster than the rate of breakdown of ES back to E + S. 4.) The velocity of the catalyzed reaction is equal to k[E][S]. 5,) The concentration of ES is small relative to the concentration of Etotal. 6.) [Etotal] >> [Efree]. 7.) The rate of reaction is equal to k[ES]. 8.) KM = [E][S]/[ES]