Periodic Remodeling in a Neural Circuit Governs Timing of Female Sexual Behavior

1. Journal Club: Vic Shao-Chih Chiang, 2021

2. PE Micevych & RL Meisel (2017) Front Syst Neurosci
• MOR: mu-opioid receptors
• Β-END: β-endorphin
• DA: dopamine
• ME: median eminence
• MPN: medial preoptic nucleus
• Nac: nucleus accumbens
• NPY-Y1: Neuropeptide & receptor
• GABA: Gamma Aminobutyric Acid
• ARH: arcuate nucleus of the hypothalmus
• VMH: ventromedial nucleus of the hypothalamus
• VTA: ventral tagmental area
• OC: optic chiasm
Female sexual
behaviour
neurocircuitry

3. MA Mittelman-Smith et al. PE Micevych (2017) Front Endocrinol
• PGR: Progesterone Receptor
• MAPK: mitogen-activated protein kinase
• CREB: cAMP response element-binding protein
• mPR: Membrane Progesterone Receptors
• PGRMC1: progesterone receptor membrane components 1
• SERBP1: serpine mRNA-binding protein 1
• cAMP: Cyclic adenosine monophosphate
Progesterone
signalling

4. M Morimoto et al. T Hara (2017) PLOS ONE
Other factors
linking
physiology
with sexual
behaviour
Blackboard Comments
• TP: testosterone propionate
• SARM-2f: selective androgen
receptor modulator-2F

5. Y Li et al. MM Luo (2016) Nat Commun
Involvement of neurotransmitters
Blackboard Comments

6. CF Yang et al. NM Shah (2013) Cell
PR+ neurons in ventrolateral VMH
• BNSTmpm: posteromedial bed
nucleus of the stria terminalis
• AVPV/POA: adjacent anteroventral
periventricular hypothalamic
nucleus / preoptic area
• MeApd: posterodorsal medial
amygdala
• VMHvl: ventrolateral ventromedial
nuclei of hypothalamus
• B-gal: beta-galactosidase
• PR: progesterone receptor

7. E Harno, EC Cottrell, A White (2013) Cell Metab
Cre-lox mice

8. Pvl neurons are active during mating
S Inoue et al. NM Shah (2020) Cell

9. LT Shuster et al. WA Rocca (2009) Menopause Int
Blackboard Comments
Effects of ovariectomy beyond changes in
progesterone & estrogen

10. TW McCullock, DM MacLean, PJ Kammermeier (2020) PLoS ONE
Experiments with additional reporter
Blackboard Comments

11. SR Kumar et al. V Gradinaru (2020) Nat Methods
Other reporters and promoters
Blackboard Comments

12. BL Roth (2016) Neuron
• hM3Dq: M3 muscarinic receptor
• hM4Di: M4 muscarinic receptor
• GsD: Gs coupled
• R165L: hM3Dq-Rq
• Β-Arr – β-arrestin
• DREADD: designer receptor
exclusively activated by designer
drugs
• hM4Dnrxn: hM4D – neurexin
• KORD: kappa-opioid receptor
• CNO: Clozapine N-oxide
Chemogenetics

13. S Inoue et al. NM Shah (2020) Cell
Pvl neurons
are required
for lordosis

14. WV Herrera-Morales, A Herrera-Solis, L Nunez-Jaramillo (2019) Arch Sex Behav
Repeated oestrous cycle on neuroplasticity
Blackboard Comments

15. CA Frye et al. JJ Paris (2020) Front Behav Neurosci
Neuropharmacology
on female sexual
behaviour
Blackboard Comments
• 3α,5α-THP: allopregnanolone

16. C Booth et al. VM Jackson (2010) J Sex Med
Neuropharmacology
on structural
connectivity
Blackboard Comments

17. CF Yang et al. NM Shah (2013) Cell
• AVPV: adjacent anteroventral
periventricular hypothalamic nucleus
• POA: preoptic area
• VMHvl: ventrolateral ventromedial
nuclei of hypothalamus
• PR: progesterone receptor
• PAG: periaqueductal gray
• Pvl: PR+ neurons from VMHvl
Pvl neuron projections to AVPV

18. S Inoue et al. NM Shah (2020) Cell
Priming drives Pvl
projections to AVPV

19. MM Sidor et al. CA McClung (2015) J Vis Exp 95
Optogenetics

20. S Inoue et al. NM Shah (2020) Cell
Optogenetics inhibition of Pvl neurons

21. SF Lau et al. NY Ip (2020) Cell Reps
Future experiments: multiphoton imaging

22. S Inoue et al. NM Shah (2020) Cell
Summary

23. TA Evans et al. SL Sabo (2019) Sci Rep
Timescale synchrony
between structural
plasticity and oestrous
Blackboard Comments

24. YJ Song et al. Y Cheng (2020) Front Neurosci
Retention of plasticity changes across
oestrous cycles
Blackboard Comments

25. TC Sudhof (2018) Neuron
Changes in
efficiency
across life to
hormone-
induced
spinogenesis
Blackboard Comments

26. YM Flores-Vivaldo et al. G Flores (2019) Synapse
Pregnancy effects on spinogenesis
Blackboard Comments
• PFC-5: layer 5 of prefrontal cortex
• Anp: adult nulliparous
• ONp: old nulliparous
• PG1: one pregnancy
• PG2: two pregnancies
• PG3, three pregnancies.

27. KE Rohr et al. J Evans (2019) Elife
Neuroplasticity with other biological cycles
Blackboard Comments

28. DH Lee, M Kume, TE Holy (2019) Science
Future experiments: PhOTseq
Supplementary Slides

29. Supplementary Slides
S Inoue et al. NM Shah (2020) Cell

30. Supplementary Slides
S Inoue et al. NM Shah (2020) Cell

31. Supplementary Slides
S Inoue et al. NM Shah (2020) Cell

32. Supplementary Slides
S Inoue et al. NM Shah (2020) Cell
Serum ACTH

33. Supplementary Slides
S Inoue et al. NM Shah (2020) Cell

34. Supplementary Slides
S Inoue et al. NM Shah (2020) Cell

35. Supplementary Slides
S Inoue et al. NM Shah (2020) Cell

36. Supplementary Slides
S Inoue et al. NM Shah (2020) Cell

37. Supplementary Slides
S Inoue et al. NM Shah (2020) Cell

38. Supplementary Slides
S Inoue et al. NM Shah (2020) Cell