Caracterización de RBP1a en busca de vacuna contra malaria por P.Vivax

1. Viviana Escobar Marín.
Biología molecular.
3 semestre, 2018-02

2. plasmodium
Genus of
unicellular
eukaryotes
Obligate
parasites of
vertebrates
and insects.
Member of the
phylum
Apicomplexa, is in
the order
Haemosporida
and family
Plasmodiidae
Destruction of
host red blood
cells can result
in disease,
called malaria.
Plasmodium vivax.
❑ It´s The most frequent and widely
distributed cause of recurring
(Benign tertian).
❑ It is less virulent than Plasmodium
falciparum, but vivax malaria can
lead to severe disease and death
due to splenomegaly
❑ P. vivax is carried by the female
Anopheles mosquito, since it is
only the female of the species
that bite.
❑ It can go dormant in the liver for
days to years, causing no
symptoms and remaining
undetectable in blood tests
❑ Hypnozoites.

3. Reticulocyte Binding Protein 1a
RBP is the reticulocyte binding protein, which is associated
with the binding protein P. Vivax Duffy the affinity of the
plasmodium vivax for reticulocytes, the RBP select the
reticulocytes for the invasion and generate the release of DBP
of the micronemas in time for the final step of union formation,
just before the invasion; this with the presence of the RBP1a
gene that has a ligand domain that is within the N-terminal
extracellular region and binds both reticulocytes and mature
erythrocytes. It is difficult to express it as a recombinant
protein because of its large size.

4. Objective.
“This study aims to functionally and
immunologically characterize
Plasmodium vivax RBP1a.”

5. Métodos.
Proteína
recombinante
Inmunizaciones
animales.
fragmentos de rRBP1a.
sangre 3 semanas después. después

6. ELISAELISA
ELISADeterminar anticuerpos anti-RBP1a.
ELISA
Citometría de
flujo.
ELISA
Diferentes fragmentos de antígeno rRBP1a
se probaron para determinar la unión a
eritrocitos humanos.

7. Electroforesis de
proteínas (SDS PAGE.)
La pureza de los antígenos se verificó por análisis
mediante electroforesis en gel de poliacrilamida.
tratamiento enzimático de
eritrocitos.
Para determinar la unión de RBP1a a los
eritrocitos tratados con enzimas.

8. Resultados.

11. Discussion.
Author. Affirmation Check
Han JH, Lee SK, Wang B,
et al.
A 34-kDa protein domain of RBP1a
(RBP1351–599) was reported as the
putative binding region, with specificity for
reticulocytes only.
The receptor for RBP1a is trypsin and
chymotrypsin sensitive but neuraminidase
resistant, an indication that this interaction
is sialic acid independent
Gupta ED, Anand G,
Singh H, et al.
Existing data from endemic regions
suggest that members of the RBP family
are naturally immunogenic
1. França CT, He WQ, Gruszczyk J,
et al. 2. Rojas Caraballo J, Delgado
G, Rodriguez R, Patarroyo MA.
3. Han JH, Li J, Wang B, et al.

12. Conclusions.
▪ This study allows us to recognize the importance of the RBP1a
gene as a target for the creation of a vaccine against plasmudium
vivax, important because a large number of people suffering from
Malaria are infected by this one, although it is not fatal is very
frequent.
▪ Malaria is caused by Plasmodium; What is often ignored is that
there are many families of this parasite, recognizing them is
essential to identify the treatment that should be given to each of
the types of Malaria.

14. Referencias.
Baquero Suárez Luis; Magíster en Ciencias, Microbiología; “Identificación y
Caracterización del Antígeno de Micronemas con Anclaje a GPI de Plasmodium
vivax (PvGAMA) y Determinación de la Unión a Reticulocitos Humanos.” 2017.
Bogotá D.C
Martínez Sánchez Lina., Vargas Grisales Natalia., Mejía Cardona Laura., Et al.
“Biología molecular”; octava ed., Escuela de ciencias de la salud Universidad
Pontificia Bolivariana.