1. Curriculum-Vitae
Vikas Choudhary Contact Information
Senior Project Fellow, Email: vkas.choudhary@gmail.com
G.N Ramachandran protein center Phone: 09216361982
IMTech (CSIR),
Chandigarh-160036, India
Career Objective
High potential Life Sciences Research Professional seeking challenging assignments in Life
Sciences research in an organization of high repute, where I can use my skills, find the
opportunity to intensify my knowledge and aptitude; and provide me excellent working
environment to help me grow and learn more.
Educational Qualifications
Examination Board/University Year of passing
M.Sc. (Biotech) Mohanlal Sukhadia University, Udaipur 2007
B.Sc. Mohanlal Sukhadia University, Udaipur 2005
Technical Skills
 Fermentation Technology: High cell density fermentation for large scale production of
recombinant proteins in E.coli. Optimization of Media and Process Parameters (by classical
and Response Surface Methodology) for Vitamins, Enzymes and Ethanol production.
Operation and maintenance of fermenter New Brunswick, Applikon, Chemap (5-15L) and
Scigenics ( 7, 14 and 400 liter).
 Downstream Processing: Preparative and Analytical column chromatography, ion-
exchange, Hydrophobic Interaction chromatography, Gel permeation, Bead-milling at
bench scale, (Batch mode), Microfiltration and Ultra filtration (Lab-scale to Large scale).
 Microbiology: Isolation, Characterization, Maintenance and Preservation of Bacterial
Cultures, Screening of Microbes for Enzyme Production, Antibiotic susceptibility etc.
 Enzyme technology: Optimization of Enzyme activity and reaction conditions for enzyme
assays of cellulase and protease. Analysis of sugars and alcohols by biochemical assays.
 Molecular techniques: Basic rDNA techniques like DNA isolation, Plasmid isolation,
restriction digestion and ligation, molecular cloning, PCR.
 Instrumentation techniques: HPLC, Electrophoresis (Agarose gel, SDS & Native PAGE),
Spectrophotometry, Aktaprime, Batch & Continuous Centrifugation, Ultracentrifugation,
Nanodrop, and Dynamic light scattering.
 Computers: MS office, MS excel, Networking and Science related software programs such
as Design Expert, Sigma Plots, MS Office, Origin, Endnote X4.
 Immunological and Biochemical diagnostics: ELISA, western blotting, collection of
blood samples and its routine biochemical tests.

2. Research Experience
Working as Senior Project Fellow in the laboratory of Dr. Ashish Ganguly, Senior Scientist,
Institute of Microbial Technology, Chandigarh, India
During my research career so far, I carried out innovative research resulting in co- and lead-
authored publications on biological important proteins and their complexes. Also, I am well
versed with fermentation of recombinant protein in E.coli and yeast Debaryomyces hansenii for
xylitol production and analysis of produced metabolites during the process by HPLC. Presently,
I am working on screening of synthetic compounds and microbial extracts capable of severing
actin to identify new drugs which can be used as therapeutics in diseases caused due to
dysregulation in actin metabolism.
My work summary includes:
 Presently working as senior project fellow in CSIR sponsored project entitled “Bugs to
drugs” at G N Ramachandran protein center under supervision of Dr. Ashish.
 Worked from Aug 2008 to Mar 2012 as project assistant on NMITLI sponsored project,
“Conversion of Cellulose and Hemicelluloses into Sugars and Alcohols” at Bioprocess
Engineering Research & Process Development Center (BERPDC), Institute of Microbial
Technology (CSIR), Chandigarh, INDIA
 Successfully carried out large scale production (400 litre) for protease production and its
downstream processing for its application in leather industry under project entitled
“Biotechnology for leather: Towards Cleaner Processing” under supervision of Dr.
Gowthaman (Scientist CLRI).
 Assisted Strides Arcolab Pvt. Limited, Hyderabad for the “Production of Recombinant
Staphylokinase from E. coli” under the supervision of Dr. D K Sahoo (Scientist).
 Learned various routine Biochemical tests during training at Department of Biochemistry
(RNT Medical College, Udaipur) from 13 May 2008 to 31 July 2008 under Supervision of Dr.
K.N Mali, Head of Biochemistry Department.
 Training at CloneGen Biotechnology Pvt. Ltd. From 15th
September, 2006 to 14th
March,
2007 under project entitled “Acetic acid and Lactic acid from indigenous food samples.”
Research Publications
1. Studies on xylitol production by metabolic pathway engineered Debaryomyces
hansenii.
Pal S1
, Choudhary V, Kumar A, Biswas D, Mondal AK, Sahoo DK. BioresourceTechnol.
2013 Nov;147:449-55.
2. Sonorensin: an antimicrobial peptide, belonging to heterocycloanthracin subfamily of
bacteriocins, from a new marine isolate Bacillus sonorensis MT93.
Chopra L1
, Singh G, Choudhary V, Sahoo DK.
Appl Environ Microbiol. 2014 May;80(10):2981-90.
3. Global shapes of F-actin depolymerization competent minimal gelsolin: insight into the role
of g2-g3 linker in pH/Ca2+
insensitivity of the first half.
Peddada, N., Sagar, A., Rathore, Y. S., Choudhary, V., Pattnaik, U. B., Khatri, N., Garg,
R., and Ashish. (2013) J Biol Chem 288, 28266-28282.
4. Visualizing the elusive open shape of G-actin in solution by SAXS data analysis.

3. Sagar, A., Peddada, N., Solanki, A., Choudhary, V., Garg, R., and Ashish. (2013)
Biochem Biophys Res Commun 435, 740-744.
5. Khatri N, Sagar A, Peddada N, Choudhary V, Chopra BS, Garg V, Garg R, Ashish:
Plasma Gelsolin Levels Decrease in Diabetic State and Increase upon Treatment with F-
Actin Depolymerizing Versions of Gelsolin. Journal of Diabetes Research 2014, 2014:8.
Personal Details
Name: Vikas Choudhary
Father’s Name: Mr. J.P. Choudhary
D.O.B: 2nd
June, 1982
Address: - 23, Hiran Magri, Sec-5
Udaipur, Rajasthan (313002)
REFEREES
Dr. Ashish (Senior Scientist)
G N Ramachandran protein center,
Institute of Microbial Technology
Chandigarh - 160036
Email: ashgang@imtech.res.in
Dr. D.K. Sahoo (Scientist F)
Biochemical Engineering and
Process development centre,
Institute of Microbial technology
Chandigarh -160036
Email: debsahoo@imtech.res.in
Dr. S.D Purohit (Professor)
Department of Biotechnology
Mohanlal Sukhadia University
Udaipur- 313002 (Rajasthan)
Email: sdp_1956@live.in