Carbon dioxide mitigation by Nannochloropsis oculata towards biodiesel production

1. Research Progress
Carbon dioxide mitigation by
Nannochloropsis oculata
towards biodiesel production
Name : Vijendren Krishnan
Chairman : Prof. Dr. Y. Uemura
(30 April 2014)

2. Taxonomy :
Nannochloropsis oculata (Hibbert)
Domain : Eukaryota
Kingdom : Chromalveolata
Phylum : Heterokontophyta
Class : Eustigmatophyceae
Family : Eustigmataceae
Genus : Nannochloropsis
Species : oculata
(Hibbert, 1981)

3. • Small spheres with diameter of 2 – 4 μM
• One single Chloroplast without pyrenoid, but
contain several lamella, each with 3
thylakoids.
• Has polysaccharide cell walls
• Do not accumulate starch
• Non-motile microalgae (non flagellate)
(Boussiba et al., 1987)
Morphology

4. • Not distinguishable by morphological
characteristics, only by molecular technique.
• Marine environment, but other species are also
found in fresh water and brackish water
(Fawley and Fawley, 2007)
• Contain pigments; Chl α, β-carotene,
xantophylls, violaxanthin and vaucherxantin
but lack chl β. (Cohen 1999)

5. Location of sampling
Marina Cove Resort
Teluk Batik Beach
Titi Panjang
(Lumut Jetty)

6. Location Coordinates
Table 1 : Location and coordinates of microalgae sampling.
Sample Location Google GPS coordinate
1 Teluk Batik Beach N 4o 11’ 20.4”, E 100o 36’ 20.8”
2 Marina Cove Resort N 4o 12’ 48.06”, E 100o 36’ 08.1”
3 Titi Panjang (Lumut Jetty) N 4o13’ 56.0”, E 100o 38’ 31.6”

7. A
B
C
Figure 1 : Growth of microalgae in F2 medium. A: 1 day, B: 4 days, C: 6 days

8. Figure 3 : Growth of microalgae in F2 medium after subculture (8 days)

9. Figure 2 : Nannochloropsis oculata Observation on microscope at
1000x magnification.

10. Day 1
Day 2
Day 3
Day 4
Day 5
Day 6

11. Day 7
Day 8
Day 9
Day 10
Day 11
Day 12

12. References
1. Hibberd, (1981). Notes on the taxonomy and nomenclature of
the algal classes Eustigmatophyceae and Tribophyceae
(Synonym Xanthophyceae). Botanical journal of the Linnean
society, 82: 93-119.
2. Boussiba, S., Vonshak, A., Cohen, Z., Avissar, I., and Richmond,
A. (1987). Lipid and biomass production by the halotolerant
microalga Nannochloropsis salina. Biomass, 12: 37-47.
3. Fawley, K. P., and Fawley, M. W. (2007). Observations on the
diversity and ecology of freshwater Nannochloropsis
(Eustigmatophyceae) with descriptions of new taxa. Protist, 158
(3) : 325 -336.
4. Cohen, Z., (1999). Chemicals from microalga, Taylor and
Francis, London, UK.