Production of glutamic acid

1. 1Pharma Knowledge

2. What is anGlutamicAcid?
Glutamic acid is an α-amino acid that is used by almost all living
beings in the biosynthesis of proteins.
It is non-essential in humans, meaning the body can synthesize
it.
It is also an excitatory neurotransmitter, in fact the most
abundant one, in the vertebrate nervous system.
It serves as the precursor for the synthesis of the
inhibitory gamma-aminobutyric acid (GABA) in GABA-ergic
neurons.

3. • Glutamate also plays an important role in the body's disposal
of excess or waste nitrogen.
• Glutamate is a key compound in cellular metabolism.
• Glutamate is the most abundant
excitatory neurotransmitter in the vertebrate nervous system.
• Glutamate also serves as the precursor for the synthesis of
the inhibitory gamma-aminobutyric acid (GABA) in GABA-ergic
neurons.
• Glutamic acid often is used as a food additive and flavor
enhancer in the form of its sodium salt, known
as monosodium glutamate (MSG).
• Auxigro is a plant growth preparation that contains 30%
glutamic acid.
functions

4. Industrial Production
• Glutamic acid is produced on the largest scale of any
amino acid, with an estimated annual production of
about 1.5 million tons in 2006.
• In 1950, Chemically synthesize by the aerobic
fermentation of sugars and ammonia in the 1950s,
with Corynebacterium glutamicum (also known
as Brevibacterium flavum).
• Isolation and purification can be achieved by
concentration and crystallization.

5. Production of Glutamic acid
Inoculum
• Corynebacteriumspp.
• C. glutamicum (prefered); C. lilum
• Brevibacterium spp.
• B. divericartum: B. alanicum
• Microbacterium spp.
• M. flavumvar. glutamicum
• Arthrobacter spp.
• A. globiformis; A. aminofaciens

6. Media
Glucose (carbon source), ammonium acetate (nitrogen source),
Corn steep liquor, Enzymatic casein hydrolysate, Dipotassium
phosphate, Mag. Sulphate, water and urea.
Bio parameters
• pH: near 7.8
• Temperature: 38°C
• Aeration: a continuous stream of sterilized air is pumped into it.
• Agitation: achieved by turbine type impellers for constant agitation.
• Time period 2-4 days
Production of Glutamic acid

7. Industrial fermentation process is carried out in three different
ways as:
1. Batch fermentation
2. Fed batch fermentation
• The fermentation process completes within 2-4
days.
• In the end of fermentation process, the broth
contains glutamic acid in the form of its ammonia
salt.
Production of Citric acid

8. Recovery
• In down stream process, the bacterial cells
are separated and broth is passed through a
basic anion exchanger.
• Glutamic acid anions get bound to the resin
and ammonia is released.
• This ammonia can be reused in fermentation.
• Glutamic acid is crystallized and dried to store.
Production of Citric acid

9. Thank you all
9
•References
*https://en.wikipedia.org/wiki/Glutam
ic_acid
*Pharmaceutical Biotecnology, Vyas
and dixit
*Biotechnology by Ashutoshkar