4. Definition
A complete blood count (CBC) ,also known
as full blood count( FBC )
, full blood exam
(FBE ro )blood panel , is a series of tests
used to evaluate the composition and
concentration of the cellular components of
blood.
Alexander Vastem is widely regarded as being
the first person to use the complete blood
count for clinical purposes in the early 1960s.
5. Haemocytopoiesis
Definition : Formation of blood
Hematopoetic Stem Cell : Origin of all blood cells
Hematopoetic Organs :
o Bone Marrow (myeloid tissue) ;
Location: long bones, ribs, sternum , vertebrae.
Primary site of blood cell formation.
T Lymphocytes are formed here and then migrate to the
lymphatic system for maturation .
o Lymphatic System;
Lymph nodes, spleen, thymus, and tonsils.
Young fetus: site of haemocytopoiesis is most of bone together
with the liver and the bone marrow .
6.
7.
8. Blood
Plasma
o Fluid Component: 90%
water .
o Solutes : 10% (electrolytes
and proteins)
Formed Elements
o Cellular component (WBC,
RBC, Plt)
9. CBC measures the following:
• The number of red blood cells (RBCs)
• The number of white blood cells (WBCs)
• The total amount of hemoglobin in the blood .
• The fraction of the blood composed of red blood cells
(hematocrit)
• The mean corpuscular volume (MCV) — the size of the
red blood cells .
• The platelet count is also usually included in the CBC.
CBC also includes information about the red blood
cells that is calculated from the other measurements:
• MCH (mean corpuscular hemoglobin).
• MCHC (mean corpuscular hemoglobin concentration).
10. Purpose:
The CBC provides valuable information about the blood and to
some extent the bone marrow, which is the blood-forming
tissue.
The CBC is used for the following purposes:
A- as a preoperative test: to ensure both adequate
oxygen carrying capacity and hemostasis.
B- to identify persons who may have an infection.
C- to diagnose anemia.
D- to identify: acute and chronic illness, bleeding
tendencies, and white blood cell disorders such as
leukemia.
E- to monitor treatment for anemia and other blood
diseases.
F- to determine the effects of chemotherapy
and radiation therapy on blood cell production.
11. Preparation:
There is no special preparation needed.
How the Test is Performed:
Blood is drawn from a vein, usually
from the inside of the elbow or
the back of the hand. The
puncture site is cleaned with
antiseptic. An elastic band is
placed around the upper arm to
apply pressure and cause the vein
to swell with blood.
A needle is inserted into the vein,
and the blood is collected in an air-
tight vial or a syringe. During the
procedure, the band is removed to
restore circulation. Once the blood
has been collected, the needle is
removed, and the puncture site is
covered to stop any bleeding.
12. In infants or young children, the
area is cleansed with antiseptic
and punctured with a sharp
needle or a lancet.
The blood may be collected in a
pipette (small glass tube), on a
slide, onto a test strip, or into a
small container.
A bandage may be applied to the
puncture site if there is any
bleeding.
13.
14.
15. White Blood Cell
Fight infection
Attack foreign material
4,500 – 11, 000 mm3 (abbreviated as 4.5
– 11)
Lifespan = hours – days
Clinical implications for an Increased
WBC (leukocytosis):
o Infection, tissue necrosis, bone marrow
malignancies, and inflammation .
Clinical implications for a Decreased
WBC (leukopenia):
o Infections, conditions or medications
that suppress or weaken the immune
system or exhaust the bone marrow .
16. WBC Differential
o Breaks down the WBC into their type (5 specific
types).
o (neutophil , basophil , monocyte , lymphocyte and
eosinophil)
o Each type is expressed as a percentage (%) of the
total WBC count.
o “Informative” component of the WBC.
o Sum of the components of the WBC differential must
add up to 100.
17. Normal Values:
NORMAL
VALUES
TEST
4.5-11.0
Adult
% (total WBC
count)
Neutrophils
3-5%
Bands
54-62%
Segs
25-33%
Lymphocytes
3-7%
Monocytes
1-3%
Eosinophils
0-0.75%
basophils
NORMAL
VALUES
TEST
X1000 cells/mm³
(µL)
Leukocyte (White
Blood Cell)
9.0-30.0
Birth
9.4-34.0
24 hours
5.0-19.5
1 month
6.0-17.5
1-3 years
5.5-15.5
4-7 years
4.5-13.5
8-13 years
18.
19. Clinical implications for neutrophilia :
o Bacterial infection, some inflammatory conditions, tissue damage, and
malignancies of the bone marrow (leukemia) .
A rise in neutrophils in general is consistent with a bacterial Infection,
A rise in bands in particular is highly suggestive of bacterial infection.
Clinical implications for neutropenia :
o Some viral conditions, overwhelming infection that exhausts the bone marrow,
cancer treatment drugs,
o certain antibiotics and psychotropic drugs, some hereditary disorders,
o Newborns with sepsis are at higher risk for developing neutropenia.
First line of defense against infection.
Two types:
oBands or stabs (3-5% ) - immature
oSegs ( 54-62% ) – mature
20. “Left Shift”
Increase in bands
Many consider it to be an increase
in the combined bands an segs.
Indicates a bacterial infection .
“Right Shift”
Technically, there is no such thing
as a right shift.
Practically, it indicates a rise in
the monocytes and lymphocytes.
Indicates a viral infection .
21. Monocytes ( 3-7% )
Second line of defense against infection .
Indicates a viral infection .
Clinical implications for monocytosis :
Monocytic leukemia, ulcerative colitis,
viral diseases such as mononucleosis and herpes
zoster,
Parasitic diseases such as Rocky Mountain Spotted
Fever and glandular fever .
Bacterial diseases ,TB ,syphilis ,malaria ,typhus.
Clinical implications for monopenia :
Some forms of leukemia, bone marrow failure or
suppression .
22. Lymphocytes (25-33 %)
Produced in the Lymphatic System .
o B Lymphocytes (B Cells): humoral immunity .
o T Lymphocytes (T cells): cell-mediated immunity .
Indicates a viral infection .
Clinical implications for lymphocytosis :
viral infections (most common),
bacterial or allergic conditions (less common) as TB ,
syphilis .
Clinical implications for lymphopenia :
Corticosteroid therapy,
adrenocortical hyperfunction,
stress,
shock .
23. Eosinophils (1-3 %)
Indicates allergic disorders and parasitic
infections .
Clinical implications for eosinophelia :
Asthma,
hay fever,
drug reaction .
Clinical implications for eosinopenia :
Corticosteroid therapy,
adrenocortical hyperfunction,
stress,
shock .
24. Basophils (0.0-0.75%)
o Indicated systemic allergic reactions
(inflammatory states) .
o Responsible for histamine release .
Clinical implications for basophilia :
Chronic inflammatory and
hypersensitivity reactions .
Clinical implications for basopenia :
Corticosteroid therapy,
adrenocortical hyperfunction,
stress,
shock .
27. Transports oxygenated Hgb to the tissues of the
body .
Contributes to maintenance of acid-base equilibrium .
Lifespan : 120 days .
Production is regulated by 2 things:
(1) Tissue oxygenation ,
(2) Renal production of erythropoietin .
Tissue hypoxia stimulates the kidneys to produce
erythropoietin, which then stimulates the bone
marrow to release RBC’s.
NB** It is the ability of the RBC’s to transport
oxygen to the tissues of the body that regulates
the production of RBC’s NOT the number of
circulating RBC’s.
28. Clinical implications for polycythemia :
Congenital heart disease,
chronic hypoxia,
high altitudes,
polycythemia vera .
Clinical implications for decreased
hemoglobin :
Renal disease,
hematological conditions involving RBC
destruction, iron deficiency, vitamin B12
deficiency,
blood loss/hemorrhage,
bone marrow suppression .
29. 3.9-5.5
Cord
4.0-6.6
1-3 days
3.9-6.3
1 week
3.6-6.2
2 weeks
3.0-5.4
1 month
2.7-4.9
2 months
3.1-4.5
3-6 months
3.7-5.3
0.5-2 years
3.9-5.3
2-6 years
4.0-5.2
6-12 years
4.5-5.3
12-18 years(male)
4.1-5.1
12-18years(female)
RBCs count
(Million/mm³)
30. RBC Indices
Measures the size and Hgb content of the RBC .
Calculated based on mathematical formulas that
reflect the relationships among the RBC, Hgb,
and Hct .
Primarily used to differentiate between different
types of anemia .
It includes :
o Mean Corpuscular Volume (MCV) ,
o Mean Corpuscular Hemoglobin (MCH) ,
o Mean Corpuscular Hemoglobin Concentration (MCHC)
o Red Cell Distribution Width (RDW) ,
o Hematocrit (Hct) .
31. Mean Corpuscular Volume (MCV)
Indicates the average size of the RBC .
Three (3) Size Descriptions:
(1) Normocytic: normal cell size (75-94µm³ );
varies with age and gender .
(2) Macrocytic: large cell size (>94µm³) .
(3)Microcytic: small cell size (<75µm³) .
Clinical implications for increased MCV:
folate or vitamin B12 deficiency,
aplastic anemia,
immune hemolytic anemia .
Clinical implications for decreased MCV:
iron deficiency anemia,
lead poisoning,
thalassemia .
33. Mean Corpuscular Hemoglobin
(MCH)
Measures average weight of Hgb
per RBC {25-33 pg (picrograms)}.
Clinical implications for increased
MCH:
same as for MCV
Clinical implications for decreased
MCH:
same as for MCV
34. MCH
ِAge
31-37 pg/cell
At birth
31-37 pg/cell
1-3 days
28-40 pg/cell
1 week – 1 month
26-34 pg/cell
2 months
25-35 pg/cell
3-6 months
23-31 pg/cell
0.5-2 years
24-30 pg/cell
2-6 years
25-33 pg/cell
6-12 years
25-35 pg/cell
12-18 years
35. Mean Corpuscular Hemoglobin
Concentration (MCHC)
Measures average concentration of Hgb per RBC .
Three (3) Hgb Concentration Descriptions:
(1) Normochromic: normal Hgb concentration (33-36%);
varies with age .
(2) Hyperchromic: increased concentration of Hgb per
RBC (>36%) .
( 3) Hypochromic: decreased concentration of Hgb per
RBC (<33%) .
Clinical implications for increased MCHC:
hereditary spherocytosis .
Clinical implications for decreased MCHC:
iron deficiency, thalassemia .
36. MCHC
Age
30-36 g Hg/dL RBC
Birth
29-37 g Hg/dL RBC
1-3 days
28-38 g Hg/dL RBC
1-2 weeks
29-37 g Hg/dL RBC
1-2 months
30-36 g Hg/dL RBC
3 months – 2
years
31-37g Hg/dL RBC
2-18 years
37. Red Cell Distribution Width (RDW)
Measures the uniformity of RBC size (11.5 – 14.5 )
Anisocytosis (increased RDW):
indicated greater cell size variability .
Clinical implications for increased RDW:
iron deficiency anemia,
folic acid deficiency anemia,
vitamin B12 deficiency anemia .
38. Hemoglobin (Hgb)
Component of the RBC that binds oxygen and
delivers it to the tissues of the body (11.5 – 14.5 g/dl,
varies based on age and gender).
Types: dependent on stage in life and an abnormalities
of the genes which regulate hemoglobin.
Composed of four (4) globin chains:
Hgb F (Fetal Hemoglobin): 2 alpha and 2 gamma chains.
Hgb A (Adult Hemoglobin): 2 alpha and 2 beta chains.
41. Hematocrit (Hct)
Percentage of packed RBC to whole blood .
Expressed the ratio of cells to blood .
The relationship between Hct and Hgb is constant/fixed.
Hct = 3 X Hgb
Hct rises and falls in the same direction and for the
same clinical reasons as does Hgb.
Clinical implications for increased hematocrit ;
More cells , Less fluid.
Dehydration,
Burns,Diarrhea,
Polycythemia vera,
Low oxygen tension (smoking, congenital heart disease, living at
high altitudes)
42. Clinical implications for
decreased hematocrit:
Fewer cells , More fluid
Anemia (various types),
Blood loss (hemorrhage),
Bone marrow failure (for example, due to
radiation, toxin, fibrosis, tumor)
Hemolysis (RBC destruction) related to
transfusion reaction,
Leukemia,
Malnutrition or specific nutritional deficiency,
Multiple myeloma,
Rheumatoid arthritis.
44. Reticulocyte (Retic Count)
o Immature RBC (0.5% - 1.5%).
o Indicates active RBC production from the
bone marrow.
o An indirect measure of hematopoiesis.
Clinical implications for increased reticulocytes
(reticulocytosis):
acute anemia,
chronic hemolytic anemia <<< (sickle cell
disease, hereditary spherocytosis)
52. Definition:
o Represent the actual number rather than the % of
neutrophils.
Clinical Implication:
o Indicates the degree of immune system functioning.
Range:
> 1000
Neutropenia:
ANC < 1000
500 – 1000: indicates a moderate risk for infection,
< 500: indicates a severe risk of life-threatening
infection.
Etiology:
Chemotherapy,
Immunosupression (Steroid Therapy),
Chronic Benign Neutropenia of Childhood,
Syndromes affecting the immune system.
53. Calculation
Three calculation methods:
Learn 1 and forget the other 2…
Method #1: (Bands + Segs)% X true WBC = ANC
Method #2: {(Bands + Segs) X WBC} / 100 = ANC
Method #3: (Bands + Segs) X (Abbreviated WBC X 10)
= ANC
54.
55. Normal range
units
Low/high
Results
Test
CBC WITH
DIFFERENTIAL
4.1 - 5.1 /
4.70 - 6.10
x 10-6/μl
3.5 (L)
Red Blood Count
12.0 - 16.0
/ 14.0 -
18.0
g/dl
10.8 (L)
Hemoglobin
37.0 - 48.0
/ 42.0 –
52.0
%
31.1 (L)
Hematocrit
140 - 415
x 10-3/μl
302
Platelets
4.5 - 11
x 10-3/μl
7.2
White Blood
Count
17 – 44
%
48 (H)
Lymphocytes
3 - 10
%
7.0
Monocytes
45 - 76
%
43 (L)
Neutrophils
0 - 4
%
2.0
Eosinophils
0 - 2
%
0
Basophils
56. Refrence interval
Units
Result
Test
5.0-10.0
x 103/mm3
1.5 L
White Blood Count
4.1-5.3
x 106/mm3
3.50 L
Red blood count
12.0-18.0
g/dL
10.8 L
Hemoglobin
37.0-52.0
%
31.1 L
Hematocrit
150-400
x 103/mm3
302
platelets
45-76
%
23L
Polys (neutrophils)
17-44
%
68 H
Lymphocytes
3-10
%
7
Monocytes
0-4
%
2
Eosinophils
0.2
%
0.6
basophils
1.8-7.8
x 103/mm3
0.34L
Polys (absolute)
0.7-4.5
x 103/mm3
1.0
Lymphocytes (absolute)
0.1-1
x 103/mm3
0.1
Monocytes (absolute)
0-0.4
x 103/mm3
0.1
Eosinophils (absolute)
0.0-0.2
x 103/mm3
0.0
Basophils (absolute)
59. 4 years female
CBC:
HB=5 gm/dl
MCV=75 fl
MCH=35 pg
WBC:150,000 /mm3
PLAT: 35,000 /mm3
Anemia
Normocytic
normochromic
Leucocytosis
Thrombocytopenia
Clinical Examination: ill
Purpura rash on the face, forearm + HSM,
generalized LN, high fever
What Do you recommend
Next Lab Investigation???
60. Case of Acute Lymphoblastic Leukemia
Bone Marrow aspiration
Lymphoblasts =80%
61.
62. 4 years male
CBC:
HB=13 gm/dl
MCV=75 fl
MCH=35 pg
WBC:15,000 /mm3
PLAT: 35,000 /mm3
Thrombocytopenia
Clinical Examination: well
ecchymosis on the face, purpura upper and lower limbs +
No HSM, No generalized LN, No fever , + preceding viral infection
What Do you recommend Next Lab
63. Case of ITP
Bone Marrow aspiration
Megakaryocytes abundunt, hyperactive, decresed budding seen (so
rapid budding cant be seen in film)
64.
65. 10 years male
CBC:
HB=5 gm/dl
MCV=75 fl
MCH=35 pg
WBC:1,000 /mm3
PLAT: 35,000 /mm3
Anemia
Normocytic
normochromic
Leucocytopeni
a
Thrombocytopeni
a
Clinical Examination:
Purpura rash all over the body, No HSM, No generalised
LN, high fever, mouth ulcers
What Do you recommend Next Lab
Investigation???
66. Case of Aplastic anemia
Bone Marrow aspiration and biopsy
Empty bone marrow. Increased fat cell and lymphocytes
67.
68. 7 years male
CBC:
HB=14 gm/dl
MCV=75 fl
MCH=35 pg
WBC:10,000 /mm3
PLAT: 200,000 /mm3
Clinical Examination:
Purpura rash on the lower limbs, buttocks + no HSM, no
generalized LN, no fever , abdominal pain, arthritis ,
hematuria
What Do you recommend Next Lab
Investigation???
Do You Recommend Bone Marrow aspiration
69. A case of
Henoch-Schönlein Purpura
(Anaphlactoid Purpura)
• Clinical Diagnosis
• Renal function evaluation
• Surest Investigation (Skin Biopsy (IgA
vasculitis))
70.
71. 5 years male
CBC:
HB=5 gm/dl
MCV=55 fl
MCH=25 pg
WBC:10,000 /mm3
PLAT: 250,000 /mm3
Anemia
Microcytic
hypochromic
Clinical Examination: pallor, jaundice , mongoloid
facies,
received blood 10 times
No Purpura + huge spleen, No generalized LN, No
fever
What Do you recommend Next Lab
Investigation???
73. Case Scenario
•
A 3 years old black male developed
painfull tender swelling of his foot
•
Temp : 39 C
•
CBC : H 8.4
•
Rbcs 3 mill / cmm
•
Hct 25
•
Mcv 83 fl
•
Mch 28 ngm
•
Mchc 33 %
74. •
T L C : 10,600 / cmm
•
Neut 72 %
•
Lymph 20 %
•
Monocyte 8 %
•
P 400 000 / cmm
•
ESR 26 / hour
•
X ray >> periosteal new bone formation
•
>> cortical destruction of the
•
fourth metatarsal bone
87. Refrences
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Guyton, A. C. & Hall, J. E. (2000). Textbook of Medical Physiology (3rd
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Hazinski, M. F. (1988). Understanding fluid balance in the seriously ill
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Jakubik, L. D., Cockerham, J., Altmann, A., & Grossman, M. B. (2003). The
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