The document reports a case of parthenogenesis in a captive Brazilian rainbow boa (Epicrates cenchria cenchria). A female boa gave birth to four offspring 59 months after being housed with a male that had undergone a vasectomy. DNA analysis of short tandem repeat markers found that the offspring were homozygous at each locus and did not possess any diagnostic alleles from the potential father. This supported that the offspring were produced through parthenogenesis rather than failed vasectomy, recanalization of the vas deferens, or prolonged sperm storage. This is the first reported case of parthenogenesis in this species of boa.
Luis Alberto Velasquez Cumplido
Grado académico: Doctor en Ciencias Biológicas, Mención Ciencias Fisiológicas
Institución: Pontificia Universidad Católica (PUC)
Fecha: 28 de Noviembre de 1997
LINEAS DE INVESTIGACIÓN
Mi foco de investigación se ha centrado en el uso de herramientas de biología molecular, celular y nanociencia para abordar problemas biomédicos básicos y aplicados. Mis proyectos básicos se centran en caracterizar las relaciones huésped hospero a nivel celular y molecular. Mis proyectos aplicados se centran en el uso de la nanopartículas para la liberación de compuestos con actividad biológica.
En los últimos 10 años he publicado 33 manuscritos en revistas con comité editorial, he participado en la presentación de 5 patentes y he presentado numerosos resúmenes en congresos nacionales e internacionales. Soy revisor de numerosas revistas nacionales e internacionales y mi laboratorio ha recibido numerosas distinciones a la excelencia científica.
Respecto a mis proyectos, soy director de Biomedicina del proyecto basal en Nanociencia y Nanotecnología CEDENNA. Me adjudique un proyecto a centros científicos de excelencia en la Wellcome trust, Inglaterra. Tengo un FONDECYT regular y un PBCT y soy subdirector de un proyecto INNOVA.
En mis proyectos colaboro con Robert Lange, del MIT, USA, con Andrew Sharkey de la Universidad de Cambridge y el Dr. Myron Cristodoulides de la Universidad de Southampton. En mi laboratorio he dirigido 14 tesis de pregrado, 4 de postgrado y un post-doctorado.
PLEASE DOWNLOAD TO SEE ANIMATION.
San Diego Research Ethics Consortium,
Salk Institute for Biological Studies.
Workshop, Parthenogenetic stem cell lines: Ethical considerations.
8 July 2011, La Jolla, CA
Luis Alberto Velasquez Cumplido
Grado académico: Doctor en Ciencias Biológicas, Mención Ciencias Fisiológicas
Institución: Pontificia Universidad Católica (PUC)
Fecha: 28 de Noviembre de 1997
LINEAS DE INVESTIGACIÓN
Mi foco de investigación se ha centrado en el uso de herramientas de biología molecular, celular y nanociencia para abordar problemas biomédicos básicos y aplicados. Mis proyectos básicos se centran en caracterizar las relaciones huésped hospero a nivel celular y molecular. Mis proyectos aplicados se centran en el uso de la nanopartículas para la liberación de compuestos con actividad biológica.
En los últimos 10 años he publicado 33 manuscritos en revistas con comité editorial, he participado en la presentación de 5 patentes y he presentado numerosos resúmenes en congresos nacionales e internacionales. Soy revisor de numerosas revistas nacionales e internacionales y mi laboratorio ha recibido numerosas distinciones a la excelencia científica.
Respecto a mis proyectos, soy director de Biomedicina del proyecto basal en Nanociencia y Nanotecnología CEDENNA. Me adjudique un proyecto a centros científicos de excelencia en la Wellcome trust, Inglaterra. Tengo un FONDECYT regular y un PBCT y soy subdirector de un proyecto INNOVA.
En mis proyectos colaboro con Robert Lange, del MIT, USA, con Andrew Sharkey de la Universidad de Cambridge y el Dr. Myron Cristodoulides de la Universidad de Southampton. En mi laboratorio he dirigido 14 tesis de pregrado, 4 de postgrado y un post-doctorado.
PLEASE DOWNLOAD TO SEE ANIMATION.
San Diego Research Ethics Consortium,
Salk Institute for Biological Studies.
Workshop, Parthenogenetic stem cell lines: Ethical considerations.
8 July 2011, La Jolla, CA
Differences in the endometrial transcript profile during the receptive period between women who were refractory to implantation and those who achieved pregnancy.
By Luis Alberto Velásquez Cumplido
Understanding of Models use for biomedical research who have similar physiological function like humans ,and the how to generate and which models are useful
Differences in the endometrial transcript profile during the receptive period between women who were refractory to implantation and those who achieved pregnancy.
By Luis Alberto Velásquez Cumplido
Understanding of Models use for biomedical research who have similar physiological function like humans ,and the how to generate and which models are useful
17-The Y-Chromosome DNA testing helps in the examination of the male.pdfANJALIENTERPRISES1
17-The Y-Chromosome DNA testing helps in the examination of the male specific portion of the
biological evidence. This is useful where small amount of male DNA is found amidst a large
amount of female DNA , as in the sexual assault cases.It can also help in the missing person
investigation as it extends the range of potential reference samples. Sine the Y chromosome is
passed on from the fathers to their sone (unchanged, except in the case of some mutations),
therefore all the males of the same paternal lineage will possess common Y chromosome
haplotype.
A set of Short tandem repeats (STR) are found on the male specific Y chromosome.The coding
genes found on the short arm of the Ychromosome are vital to male sex determination,
spermatogenesis andother male related functions. The Y-STRs are polymorphic among the
nonrelated males.
Sometimes when sepoarating the mal ecomponent and the female component becomes difficult,
then the YSTRs help in the determination. Since The Y-STRs are not present in the females,
therefore in a case where the male and female are involved, it becomes clear that as the Y-STRs
are present the male component is easily detected as only this part will be amplified in the PCR.
The YSTRs are also helpful when a number of males are involved. As regular STRs cause
masking effect the YSTR will help to identify all the males who have contributed to the
evidence.
ADVANTAGES-
1. extremely discriminating.
2. Extremely sensitive.e. low amounts of DNA can be used for determination.
3. Not onlu body fluids, but alsp touched materials or handled surfaces, where skinn cells ahev
been left can be used for DNA profiling.
4. Multiple testing systems ar available, which increases the likelyhood of successfully
generating DNA profiles.
5. Apart from standart=d STR enem mt DNA or YSTRs are used for profiling.
6. With the presence of the current technologies, from amidst mixed DNA samples, the DNA of
a particular individual can be profiled.
LIMITATIONS
1. Random match probability cannot be excluded.
2. Sometimes even when crime is not committed, we do get ,low levels of DNA on surfaces.
3.The important question while dealing with low DNA quantity is that whether the DNA that
was deposited was deposited during the crime or at some other time.
4.Searching evidence is challenging.
5.Sometimes finding the evidence is altogether impossibl.
6. In case of mtDNA /T STRs , as they are passed on from generation to generation either from
fathers to sons or mothers to daughters and son, therefore sometimes unrelated persons are also
share the same profile.
7. DNA mixtures are very complex.
8.Interpretation is required in many cases.
16- from Cyanobacterial to humans, many terristrial organisms have acquired the circadian
rhythms to adapt to sunlight to increase their survival rate.Circadian Rhythm is a biological clock
that displays an endogenous, entrainable oscillation of 24 hours.Thesse oscillations are driven by
circadian clocks.
PULSE CHASE EXPERIME.
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Molecular genetic analysis and ecological evidence on thynnine waspsNurfarah Ain
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Urologist and male fertility doctor for vasectomy and vasectomy reversal, sperm retrieval, testicular mapping, varicocele repair and ejaculatory duct repair, Dr. Paul Turek, speaks about Fertility Restoration after Cancer: Current and Future Therapies. Dr. Turek is director of The Turek Clinic. Located in San Francisco, California, The Turek Clinic provides world-class patient care with an essential holistic approach. (WARNING: Images in slides not appropriate for all audiences due to subject matter.)
Diversification of the genus Dolichothele (Araneae: Theraphosidae) in the Bra...WendyArroyo8
Wendy Y. Arroyo-Pérez1,2, Vera Nisaka Solferini2
1Genetics and Molecular Biology Program, University of Campinas, Brazil.
2Department of Genetics, Evolution, Microbiology and Immunology – University of Campinas, Brazil
*vera.solferini@gmail.com
*wendyarroyo2015@gmail.com
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2. Parthenogenesis in a Brazilian Rainbow Boa 173
No DNA was collected from offspring produced prior to
vasectomy. Twenty-seven months following vasectomy of
the male, the female gave birth to two live snakes. DNA
was not isolated from these two snakes. Offspring (three
live and one stillborn) were born 59 months following vasectomy of the male. The three viable offspring were determined to be female using cloacal probing at 30 months of
age. Briefly, a probe was inserted caudally into the cloacal
opening after sterile lubrication. In all snakes, the depth of
the probe was equal to or less than three subcaudal scutes,
which is consistent with probe depth previously described
for female rainbow boas (E. cenchria) (three scutes) [Ross
and Marzec, 1990].
The male snake was found dead in the enclosure
16 months after the birth of the four offspring. Gross
necropsy and histopathology revealed the cause of death
to be chronic cardiac disease. Histological examination of
the vas deferens demonstrated fibrosis, complete occlusion, and cystic changes bilaterally. No evidence of vas
deferens recanalization was found after examination of
multiple tissue sections. Marked testicular atrophy and
minimal spermatogenesis was present.
DNA Isolation
DNA was isolated from a whole blood sample obtained from the tail vein of the adult male and female
snakes following the nucleated erythrocyte protocol, using DNeasy Blood and Tissue Kit (Qiagen Inc., Valencia,
CA). A 3-mm segment of tail was collected from the stillborn snake and the first shed skin was collected from the
three viable offspring. A 1-cm segment of shed skin from
each viable offspring was minced and used for DNA isolation. All tissue (one tail tip and three shed skin) samples
from the four offspring were digested overnight following
the tissue extraction protocol of the DNeasy Blood and
Tissue kit. To remove any undigested scales, the digested
tissue was centrifuged prior to application of the solution to the wash column. To compensate for low yield,
DNA isolated from shed skin was concentrated prior to
polymerase chain reaction (PCR) amplification using Genomic DNA Clean and Concentrator Kit (ZYMO Research Corp., Irvine, CA).
STR Amplification
Thirty-nine STR markers previously characterized
in three genera of boids were used for analysis [Matson
et al., 2001; Ramanana et al., 2009; Tzika et al., 2008].
Forward primers were modified with a 5 addition of
a sequence specific to a dye-labeled primer. Using approximately 10 ng of template DNA for each sample, the
following 20 μl PCR reaction conditions were followed:
1 pmol of each dye-labeled primer and STR-specific reverse primer, 0.1 pmol each STR-specific forward primer,
2.0 mM dNTP, 2.0 mM MgCl2 , 0.5% BSA, 1X PCR buffer
II, and 0.375 U AmpliTaq polymerase (Applied Biosys-
tems, Foster City, CA). Each STR was amplified in a
separate reaction using the following temperature profile:
3-min incubation at 94◦ C followed by 35 cycles of denaturation at 94◦ C for 30 sec, annealing at 55◦ C for 20 sec,
and extension at 72◦ C for 45 sec. The amplification was
followed by a final extension step of 30 min at 72◦ C to
ensure that all products were full length. All PCR amplification was performed on a GeneAmp PCR Systems
9700 (Applied Biosystems). Products were individually
size separated on an ABI 3730 DNA analyzer and exact
fragment sizes were determined using STRand analysis
software [Toonen and Hughes, 2001].
RESULTS
Thirty-nine STRs were tested for analysis. Eighteen
STRs failed to produce products or generated nonspecific
products. Twelve were homozygous in all samples and
therefore nondiagnostic. The alleles for STR marker μsat
3 were uninformative for this male-female pairing and the
male shared an allele with the female for μsat 24 that
did not segregate in the offspring. The remaining nine
STRs had diagnostic differences between the two adults
that would have been present in the offspring with proper
segregation (Table 1). All offspring were homozygous for
all STR loci, always an allele present in the female snake.
In no instance did any offspring possess a diagnostic allele
from the sire. Offspring 1 possessed four markers that
displayed only the maternal allele that was not present
in the male, offspring 2 had seven exclusionary markers,
offspring 3 and the nonviable offspring each had three
markers with only a maternal allele.
DISCUSSION
Surgical vasectomy was first reported in snakes (two
garter snakes (Thamnophis sirtalis radix) in 1979 [Zwart
et al., 1979]. In all species, following identification of the
vas deferens, the procedure is relatively simple and involves bilateral removal of a portion of the vas deferens
with ligation or plugging of the remaining vas deferens. If
anatomy is not correctly identified, inadvertent removal
of non-vas deferens tissue is possible, which may result
in continued male fertility. In this case, histopathologic
examination of the tissue removed at the time of the vasectomy verified removal of two 3–4 mm segments of
tissue composed of fibrous tissue with multiple profiles
of tubules lined by pseudostratified columnar epithelium
surrounded by smooth muscles, consistent with vas deferens and epididymis.
Vas deferens recanalization has not been reported in
reptilian species. However, in humans postvasectomy recanalization has been well studied. In general, recanalization is extremely rare and may represent granuloma formation with multiple small proliferating epithelial lined
channels containing spermatozoa [Haldar et al., 2000].
Zoo Biology
3. 174 Kinney et al.
TABLE 1. STR genotypes of potentially parthenogenic Brazilian rainbow boas
STR
μsat 1
μsat 3
μsat 11
μsat 24
55HDZ314
5HDZ277
55HDZ554
55HDZ602
55HDZ617
Male
314
213
274
272A
184A
204
180
214A
218A
318A
229
274
292
184A
208A
182A
214A
220
Female
314
213
274
292
183B
204
176B
204B
220
Offspring 1
364B
229
278B
292
183B
210B
180
212B
222B
314
213
274
292
183B
210B
176B
204B
220
314
213
274
292
183B
210B
176B
204B
220
Offspring 2
364B
213
278B
292
183B
210B
176B
204B
222B
Offspring 3
Offspring 4
(dead)
364B
213
278B
292
183B
210B
176B
204B
222B
314
229
274
292
183B
204
180
212B
222B
314
229
274
292
183B
204
180
212B
222B
364B
213
274
292
183B
204
180
212B
220
364B
213
274
292
183B
204
180
212B
220
288
164
208
154
222
226
180
184
182
222
368
190
288
164
208
154
222
226
180
184
182
222
368
190
288
164
208
154
222
226
180
184
182
222
368
190
288
164
208
154
222
226
180
184
182
222
368
190
288
164
208
154
222
226
180
184
182
222
368
190
Loci with no diagnostic value
5HDZ229
5HDZ317
5HDZ360
5HDZ667
55HDZ11
55HDZ220
55HDZ302
55HDZ314
55HDZ339
55HDZ557
55HDZ56
55HDZ600
288
164
208
154
222
226
180
184
182
222
368
190
288
164
208
154
222
226
180
184
182
222
368
190
288
164
208
154
222
226
180
184
182
222
368
190
288
164
208
154
222
226
180
184
182
222
368
190
288
164
208
154
222
226
180
184
182
222
368
190
288
164
208
154
222
226
180
184
182
222
368
190
288
164
208
154
222
226
180
184
182
222
368
190
Twenty-one STRs provided robust amplification products for analysis. Bolded types were diagnostically informative. A = female
exclusionary alleles (alleles present only in the male), B = male exclusionary alleles (alleles present only in the female). STR = short
tandem repeats.
In one study of 870 vasectomized men, 50 (5.7%) had
at least one post vasectomy semen analysis that showed
motile sperm, which the authors considered evidence of
recanalization [Labrecque et al., 2003]. It is important
to note that while early (<6 month) or late (>6 month)
recanalization is a considerable risk, the pregnancy rate
after vasectomy is about 1 in 2,000 (0.05%) likely due to
reduced number of sperm in the ejaculate [Philp et al.,
1984]. Upon the death of the potential sire in the case
described in this report, recanalization of the vas deferens with subsequent male fertility was ruled out. Gross
necropsy and histopathological evaluation concluded that
the vas deferens was disrupted with adhesions (to the regional fat pads), fibrosis, and cystic changes bilaterally.
The vas deferens were both occluded and testicular atrophy with minimal spermatogenesis was noted in both
testicles. Therefore, recanalization of the vas deferens following vasectomy was eliminated as a possible mechanism
of reproduction in the case reported here.
Prolonged sperm storage has been documented in a
number of snake species [Birkhead and Møller, 1993; Fox,
1976; Stewart, 1972]. Seven years and six months is the
longest reported time period of suspected sperm storage in
a snake [Magnusson, 1979]. However, in most reports of
suspected prolonged sperm storage, parthenogenetic reproduction was not excluded as a mechanism for offspring
production. A number of hypotheses have been proposed
regarding the function of prolonged sperm storage includ-
Zoo Biology
ing; separation of reproductive events (copulation and fertilization) to optimize the timing in both sexes, insurance
against not finding a partner (especially in species with a
low likelihood of encountering members of the opposite
sex), and enhanced opportunities for sperm competition,
as sperm storage could extend the time period over which
sperm from different males overlap in the reproductive
tract [Birkhead and Møller, 1993]. In the present case, the
male and female were housed in the same enclosure prior
to vasectomy. Vasectomy was performed approximately
five years prior to birth of the four offspring making sperm
storage unlikely. To definitively exclude prolonged sperm
storage, genetic analysis was required to demonstrate the
lack of male genetic contribution to the offspring.
STR genotypes supported offspring parthenogenesis. There were two possible parents but no female exclusionary alleles (alleles present only in the potential
sire) present in any of the offspring. The lack of the male
only alleles and the observed allele homozygosity of the
STR markers in the offspring support derivation from a
parthenogenetic event.
Parthenogenesis has been described in a variety of
vertebrates including captive fish, amphibians, reptiles,
birds, and mammals [Bartelmez and Riddle, 1924; Booth
et al., 2011a, 2011b; Chapman et al., 2007; Feldheim et al.,
2010; Groot et al., 2003; Kono et al., 2004; Lenk et al.,
2005; Olsen and Marsden 1954; Robinson et al., 2011;
Sarvella, 1973; Schut et al., 2008; Smith et al., 2000;
4. Parthenogenesis in a Brazilian Rainbow Boa 175
Spurway, 1953; Watts et al., 2006]. A necessary requirement for viable parthenogenetic reproduction is the maintenance of ploidy levels of offspring. Three mechanisms
have been described to maintain ploidy levels; apomixis,
premeiotic doubling of chromosomes, and automixis
[Lampert, 2008]. In the absence of mutations, apomixis
and premeiotic doubling of chromosomes produces offspring genetically identical to the mother. In the present
case, the heterozygous state of the female is not observed
at any of the STR in the offspring, thus excluding apomixis
and premeiotic doubling. Automixis can produce genetically variable offspring because of segregation and recombination of nonidentical homologous chromosomes.
The degree of variability depends on the exact mechanism
[Lampert, 2008]. Automixis is the only reported mechanism of parthenogenesis in reptilian species [Lampert,
2008]. Apomixis (oocyte production by mitosis) and premeiotic doubling (genome doubling prior to meiosis) in
a Burmese python could not be excluded but was not
proven [Groot et al., 2003]. In the ZW heterogametic sex
determination system, automixis has the potential to result in male or female progeny. If automictic terminal
fusion (fusion of the second polar body with the egg nucleus during meiosis) occurs, male (ZZ) or female (WW)
progeny can result. Until recently, it was thought that female WW vertebrates were nonviable, however a recent
report in a Boa Constrictor (Boa constrictor imperators)
suggests WW females produced from terminal fusion automixis can be viable [Booth et al., 2011a]. In contrast to
terminal fusion, central fusion (fusion of the first polar
body with the oocyte during meiosis) results in female
(ZW) only progeny and maintains maternal heterozygosity [Lampert, 2008]. In the present study, the sex of all
three viable offspring was determined to be female using
cloacal probing. Offspring produced in this case report
are either ZW or WW. Because maternal homozygosity
is maintained in the offspring, automictic terminal fusion
with production of WW progeny is considered most likely.
Parthenogenesis, a rare event with unknown evolutionary significance, has fascinated scientist for a number of years. To the authors’ knowledge, parthenogenetic
reproduction in vertebrates has only been documented
in captive populations with females isolated from males
for an extended period of time. Recently, parthenogenesis has been identified as a possible problem for genetic
management of Komodo dragons (Varanus komodoensis)
[Watts et al., 2006]. Parthenogenisis has the potential to
bias the sex ratio (depending on both the mechanism used
to maintain ploidy levels and gametic sex determination
system of the species) and instantaneously results in homozygosity of the genome, which results in an increased
risk for lower overall fitness [Watts et al., 2006]. Reproductive plasticity has been confirmed in Komodo dragons
using genetic fingerprinting. In a single case, a female was
documented to reproduce asexually through parthenogenesis and later sexually after the introduction of a male.
This observation led to the concern that housing female
Komodo dragons in exhibits without males could induce
parthenogenesis with potential negative impacts on the
population’s genetic pool. The present case is the first to
document parthenogenesis in the presence of a conspecific vasectomized male. This finding is significant as it
can no longer be assumed that the presence of a male prevents parthenogenesis in all species. Unfortunately, the
offspring that were produced prior to vasectomy of the
male are unavailable for genetic analysis and therefore we
can not determine if this female reproduced parthenogenetic offspring while being housed with a sexually capable male. We are also unable to document reproductive plasticity, as the mechanism of reproduction (sexual
vs. parthenogenic) for the offspring produced prior to the
vasectomy of the male was not determined. The documentation of parthenogenesis in an individual housed with a
vasectomized conspecific is important, as it has the potential to influence housing decisions in species that have
breeding recommendations to promote genetic diversity
within a population. Genetic fingerprinting of offspring
produced within populations of endangered species may
be needed to maintain maximum genetic diversity as it
may not be accurate to assume offspring produced by
pairs are the result of sexual reproduction.
CONCLUSIONS
1. Parthenogenesis, most likely as a result of automictic
terminal fusion, was demonstrated for the first time
in the Brazilian rainbow boa (E. cenchria cenchria)
through analysis of STR genotypes.
2. Incomplete vasectomy of the male cagemate, recanalization of the vas deferens, and sperm storage were
excluded using histopathology and genetic analysis.
3. This report of parthenogenesis in an individual housed
with a conspecific male, calls for more complete investigation into the triggers of parthenogenesis and the
potential genetic consequences of parthenogenesis for
captive populations.
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