This document is a curriculum vitae for Dr. Yu Zhou. It lists his contact information, professional experience as an instructor and postdoctoral associate at Washington University School of Medicine since 2010, education including a Ph.D. in Neuroscience from SUNY Buffalo in 2005, professional affiliations, honors, bibliography of 10 peer-reviewed publications as first author or corresponding author between 2005-2015, and three professional references.
Professor Stephen Simpson, Academic Director, Charles Perkins Centre, School of Biomedical Sciences, The University of Sydney. http://www.garvan.org.au/news-events/leaders-in-science-and-society
Professor Stephen Simpson, Academic Director, Charles Perkins Centre, School of Biomedical Sciences, The University of Sydney. http://www.garvan.org.au/news-events/leaders-in-science-and-society
Enhancing Rare Disease Literature for Researchers and PatientsErin D. Foster
Objectives: In rare disease research, structured phenotype information is crucial to document in order to draw connections between other known cases and work towards diagnosis and treatment of disease. The Human Phenotype Ontology (HPO) is a standardized vocabularly that describes phenotypic abnormalities encountered in human diseases. To enable the increased identification of traits (i.e., phenotypes) associated with rare diseases, the HPO was expanded to include layperson synonyms to make the ontology more accessible and useful to patients. Additionally, the HPO was used to annotate phenotypes in a sample of rare disease case reports to provide structured annotations of rare disease phenotypes.
Methods: The HPO was systematically reviewed and 'layperson synonyms' were added to include terms used by patients and non-medical professionals. Subsequent work annotated phenotypic descriptions in a sample of rare disease case reports with HPO terms. The literature sample was identified by filtering 'case reports' in PubMed and excluding articles that were already included in the Online Mendelian Inheritance of Man (OMIM) database. The sample set was further restricted to articles from the European Journal of Human Genetics for the pilot set, which resulted in a final sample size of 143 articles. The papers were reviewed and annotated for the following information: disease name, associated gene(s), and corresponding phenotypes.
Results: The review of the HPO resulted in approximately half of the terms including layperson synonyms. A subset of the literature sample was annotated to determine the best curation workflow. Of that subset of papers (n=20), 353 total phenotypes were identified. 12% of these phenotypes were not included in the HPO and required new term and/or synonym requests. Some challenges encountered in this work included maintaining consistency in HPO term definitions and use, as well as annotation reliability.
Conclusion: This work contributes to knowledge of rare diseases by curating the existing literature to provide structured annotation of rare disease traits, which helps with information retrieval and data interoperability and reuse. Additionally, the expansion of the HPO to include layperson synonyms enables patients to 'self-phenotype' and contribute to the identification of rare disease traits. Following the completed annotation of the literature sample, future work will focus on incorporating the annotations into databases that collect rare disease phenotypic information. Further work is also being done to add additional layperson synonyms to the HPO through review of patient forums and medical message boards to continue to identify terminology used by actual patients.
Enhancing Rare Disease Literature for Researchers and PatientsErin D. Foster
Objectives: In rare disease research, structured phenotype information is crucial to document in order to draw connections between other known cases and work towards diagnosis and treatment of disease. The Human Phenotype Ontology (HPO) is a standardized vocabularly that describes phenotypic abnormalities encountered in human diseases. To enable the increased identification of traits (i.e., phenotypes) associated with rare diseases, the HPO was expanded to include layperson synonyms to make the ontology more accessible and useful to patients. Additionally, the HPO was used to annotate phenotypes in a sample of rare disease case reports to provide structured annotations of rare disease phenotypes.
Methods: The HPO was systematically reviewed and 'layperson synonyms' were added to include terms used by patients and non-medical professionals. Subsequent work annotated phenotypic descriptions in a sample of rare disease case reports with HPO terms. The literature sample was identified by filtering 'case reports' in PubMed and excluding articles that were already included in the Online Mendelian Inheritance of Man (OMIM) database. The sample set was further restricted to articles from the European Journal of Human Genetics for the pilot set, which resulted in a final sample size of 143 articles. The papers were reviewed and annotated for the following information: disease name, associated gene(s), and corresponding phenotypes.
Results: The review of the HPO resulted in approximately half of the terms including layperson synonyms. A subset of the literature sample was annotated to determine the best curation workflow. Of that subset of papers (n=20), 353 total phenotypes were identified. 12% of these phenotypes were not included in the HPO and required new term and/or synonym requests. Some challenges encountered in this work included maintaining consistency in HPO term definitions and use, as well as annotation reliability.
Conclusion: This work contributes to knowledge of rare diseases by curating the existing literature to provide structured annotation of rare disease traits, which helps with information retrieval and data interoperability and reuse. Additionally, the expansion of the HPO to include layperson synonyms enables patients to 'self-phenotype' and contribute to the identification of rare disease traits. Following the completed annotation of the literature sample, future work will focus on incorporating the annotations into databases that collect rare disease phenotypic information. Further work is also being done to add additional layperson synonyms to the HPO through review of patient forums and medical message boards to continue to identify terminology used by actual patients.
Handbook of Andrology
Contents
PREFACE
B. Robaire and P. Chan
LIST OF CONTRIBUTORS
FOREWORD
P. Troen
1. What are the components of the male reproductive system?
CNS, pituitary, testis, epididymis, prostate, seminal vesicles, scrotum,
penis
K.P. Roberts
2. What is the relationship among the various endocrine components
of the male reproductive system?
Hypothalamic-Pituitary-Testicular Axis, feedback loops
R. Swerdloff
3. How are communication signals read in the male reproductive
system?
Receptors for gonadotropins and androgens
I. Huhtaniemi
4. What compounds mediate communication within the testis? Where
and how are male-associated hormones produced?
Integration of the hypothalamus, pituitary and testis
B.R. Zirkin
5. How is the synthesis of testosterone regulated?
V. Papadopoulos
6. How are germ cells produced and what factors control their
production?
M. Nagano
7. What unique chromosomal events lead to the formation of a
haploid male germ cell?
M.A. Handel
8. How is the production Hormonal regulation and FSH
R.I. McLachlan
9. Are there epigenetic formation? What is cells?
Imprinting, DNA methylation
J.M. Trasler
10. What does the epididymis B.T. Hinton
11. What is the prostate G.S. Prins
12. How does semen analysis status of the male?
C. Wang
13. How is sperm chromatin doing such assessments?
Range of methods B. Robaire
14. What is sperm banking? in humans? Animals?
Sperm banking, consequences practice
S.A. Rothmann
15. How does the sperm fertilization take place?
Capacitation, acrosome egg activation
J.P. Evans and J.16. What determines maleness?
Sex determination, phenotype
D.W. Silversides and
XI
system?
vesicles, scrotum,
endocrine components
loops
reproductive
the testis? Where
control their
formation of a
8. How is the production of spermatozoa regulated?
Hormonal regulation of spermatogenesis: role of androgens
and FSH
R.I. McLachlan
9. Are there epigenetic events associated with male germ cell
formation? What is the role of genomic imprinting in male germ
cells?
Imprinting, DNA methylation
J.M. Trasler
10. What does the epididymis do and how does it do it?
B.T. Hinton
11. What is the prostate and what are its functions?
G.S. Prins
12. How does semen analysis assist in understanding the reproductive
status of the male?
C. Wang
13. How is sperm chromatin structure assessed? What is the value of
doing such assessments?
Range of methods that assess sperm chromatin quality
B. Robaire
14. What is sperm banking? When and how is it (or should it be) used
in humans? Animals?
Sperm banking, consequences of its use in animal and clinical
practice
S.A. Rothmann
15. How does the sperm make its way to the egg and how does
fertilization take place?
Capacitation, acrosome exocytosis, steps of sperm-egg interaction,
egg activation
J.P. Evans and J.L. Bailey
16. What determines maleness?
Sex determination, testis formation and the development of the male
phenotype
1. Curriculum Vitae – Yu Zhou, Ph.D.
7/6/2015
Contact information:
Office P.O. Box 8054
Department of Anesthesiology
Washington University School of Medicine
660 South Euclid Avenue
St. Louis, MO 63110
Home: 10823 Chase Park Lane, Apt. C
St. Louis, MO 63141
Telephone: (314) 800-8187 (Mobile)
(314) 362-8559 (Office)
Email: zhouy@wustl.edu
Professional Experience::
Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO
Instructor 2010 - present
Postdoctoral Associate 2005 - 2010
Education:
State University of New York at Buffalo, Buffalo, N.Y.
Ph.D. in Neuroscience 2005
Zhejiang University, Hangzhou, Zhejiang, China,
M.S. in Biophysics 1999
Wuhan University, Wuhan, Hubei, China,
B.S. in Biochemistry 1996
Professional Societies and Organizations:
Biophysical Society
Society for Neuroscience
2. Yu Zhou, Ph. D. Curriculum Vitae, p. 2
7/6/2015
Honors and Awards:
Zhejiang University Guanghua Scholoar's Award 1997
Bibliography:
1. Zhou, Y. (corresponding author), Xia, X.-M., Lingle, C.J. Cadmium-cysteine coordination in the BK
inner pore region and its structural and functional implications, Proc Natl Acad Sci U S A. 2015 Apr 6.
pii: 201500953;
2. Zhou, Y. and Lingle, C.J. Paxilline inhibits BK channels by an almost exclusively closed channel block
mechanism, Journal of General Physiology 2014; 144:415-440 (commented by Dr. McManus and Dr.
Rothberg);
3. Brenker, C., Zhou, Y., Müller, A., Echeverry, FA.,Trötschel, C., Poetsch, A., Xia, XM., Bönigk, W.,
Lingle, CJ., Kaupp, UB., Strünker, T., The Ca2+
-activated K+
current of human sperm is mediated by
Slo3, eLife, 2014 Mar 26;3:e01438. doi: 10.7554/eLife.01438.;
4. Zhou, Y., Zeng, X.-H., and Lingle, C.J. Barium ions selectively activate BK channels via the Ca2+
-bowl
site. Proc Natl Acad Sci U S A. 2012 Jul 10; 109(28):11413-8;
5. Yang, C., Zeng, X.-H., Zhou, Y., Xia, X.-M., and Lingle, C.J. LRRC52, a testis-specific auxiliary
subunit of the alkalization-activated Slo3 channel. Proc Natl Acad Sci U S A. 2011 Nov 29;
108(48):19419-24;
6. Zhou, Y. (corresponding author), Xia, X.-M., and Lingle, C.J. Cysteine scanning and modification
reveal major differences between BK channels and Kv channels in the inner pore region. Proc Natl
Acad Sci U S A. 2011 Jul 19; 108(29):12161-6;
7. Zhou, Y. (corresponding author), Xia, X.-M., and Lingle, C.J. Inhibition of large-conductance Ca2+
-
activated K+
channels by nanomolar concentrations of Ag+
. Molecular Pharmacology 2010
Nov;78(5):952-60;
8. Zhou, Y., Tang, Q.-T., Xia, X.-M., and Lingle, C.J. Glycine311, a determinant of paxilline block in BK
channels: a novel bend in the BK S6 helix. Journal of General Physiology 2010; 135:481-94;
9. Zhang, Z., Zhou, Y., Ding, J.-P., Xia, X.-M., and Lingle, C.J. A limited access compartment between
the pore domain and cytosolic domain of the BK channel. Journal of Neuroscience 2006; 26:11833-
11843.
10. Zhou, Y., Auerbach A. Gating reaction mechanisms for NMDA receptor channels. Journal of
Neuroscience 2005; 25:7914-23
11. Zhou, Y., Pearson J.E., Auerbach A. Phi-value analysis of a linear, sequential reaction mechanism:
theory and application to ion channel gating. Biophys. J. 2005; 89:3680-5 (commented by Dr.
Colquhoun);
12. Shen Y., Zhou Y., Yang XL. Characterization of AMPA receptors on isolated amacrine-like cells in
carp retina. Eur. J. Neurosci. 1999; 11:4233-40;
Peer Reviews:
ACS Chemical Biology
Biophysical Journal
3. Yu Zhou, Ph. D. Curriculum Vitae, p. 3
7/6/2015
Journal of General Physiology
Pflügers Archiv European Journal of Physiology
4. Yu Zhou, Ph. D. Curriculum Vitae, p. 4
7/6/2015
References:
1. Professor Christopher Lingle
Washington University, School of Medicine
St. Louis, MO 63110
Email: clingle@wustl.edu
Office Phone: 314-362-8558
FAX: 314-362-8571
2. Professor Joseph Henry Steinbach
Washington University, School of Medicine
St. Louis, MO 63110
Email: jhs@wustl.edu
Office Phone: 314-362-8560
FAX: 314-362-8571
3. Professor Anthony Auerbach
Center for Single Molecule Biophysics
State University of New York at Buffalo
Department Of Physiology and Biophysics
324 Cary Hall, South Campus
3435 Main Street
Buffalo, NY 14214
Email: auerbach@buffalo.edu
Office Phone: 716-829-2435
FAX: 716-829-2569