RRIDs were developed to enhance rigor and transparency in research, supporting NIH guidelines for the better identification of resources like antibodies and cell lines. They help institutions track resources, aid compliance with guidelines, and serve as a warning system for resource quality. The document emphasizes the collaborative effort in promoting RRIDs and provides resources for authentication and further information.

1. Why should my institution
support RRIDs?
RRIDs were created jointly by NIH, Journal Editors, UCSD and other researchers
and function in support of NIH guidelines for Rigor and Transparency
RRIDs are funded and maintained jointly by the following:
U24-DA039832 to The Neuroscience Information Framework, U24-DK097771 to
dkNet, R43-OD024432 to SciCrunch Inc, and Leona M. and Harry B. Helmsley
Charitable Trust

2. Reproducibility woes touch all aspects of the
research enterprise

5. New NIH
Guidelines affect
all grants
(2016/2017)
NIH Rigor and
Transparency Guidelines
describe changes to grant
review guidelines,
including an explicit call to
improve use of:
Antibodies,
Cell Lines,
Organisms,
and Chemicals
What is the annual budget from NIH?

6. Freeman et al, 2017. Reproducibility2020: Progress and priorities
*

7. Why these reagents and
not all reagents?

8. How do you authenticate an antibody?
Nature Methods, 2016: A proposal for validation of antibodies
Mathias Uhlen1, Anita Bandrowski2, Steven Carr3, Aled
Edwards4, Jan Ellenberg5, Emma Lundberg1, David L Rimm6,
Henry Rodriguez7, Tara Hiltke7, Michael Snyder8 & Tadashi
Yamamoto9
These pillars rest on
proper identification

9. Who authenticates every antibody?
There are 5,700 journals
indexed in PubMed

10. 34 journals say they
require cell line
authentication, others
enforce identification

11. At the simplest level every study is like a recipe,
resources (ingredients) and a protocol

12. •
•

13. •
•
•
•

14. The State of The Art in Publishing

16. Papers are still poor at
identifying the simplest
part of the paper, the
materials used, but
when asked the authors
know what they used
so this is culture not
malice!
Vasilevsky 2013

18. Anatomy of the RRID syntax
NeuroMab Cat# 75-028 Lot#45, RRID:AB_2307331
Company or
Provider
Local
Identifier
Version or
Lot used
Global
Identifier

19. http://rrid.site

20. PubMed ID / DOI
I used this mouse
RRID registered
I made this antibody
I am a UC researcher

21. •
•
•
•

24. -eLife Blog

26. RRIDs in the wild

30. But why should my institution care?

31. 1.
RRIDs help researchers demonstrate
they comply with NIH guidelines

32. http://doi.org/10.6075/J0RB72JC

33. 2.
RRIDs enable an early warning system
for bad resources

35. 3.
RRIDs enable UC to track resources as
intellectual output of faculty/staff

37. To recommend that researchers use RRIDs

38. Useful links for RRIDs
Resource authentication example document:
https://library.ucsd.edu/research-and-collections/data-curation/_files/ExampleAuthenticationKeyBiologicalChemicalResources201609b.pdf
Here are the people pushing RRIDs:
Here are 2 FOAs that mention RRIDs
https://grants.nih.gov/grants/guide/rfa-files/RFA-DA-18-014.html
https://grants.nih.gov/grants/guide/rfa-files/RFA-DK-17-010.html
Cell Press, eLife etc instructions to authors that we have found so far
https://docs.google.com/spreadsheets/d/1cGtY2oIMfBzg6MKei_S2niCbu0Cici2n17dZNLgBdNw/edit#gid=1245999377
External Resolvers back RRIDs
n2t.net/RRID:AB_90755 (California Digital Library)
identifiers.org/RRID/RRID:AB_90755 (European Bioinformatics institute)
Companies, vendors, random projects etc
Cellosaurus: web.expasy.org/cellosaurus/CVCL_2260
MMRRC: https://www.mmrrc.org/catalog/sds.php?mmrrc_id=32045
BioLegend: https://www.biolegend.com/en-us/products/purified-anti-akt1-antibody-12289
NeuroMab: http://neuromab.ucdavis.edu/catalog.cfm
Hypothes.is https://web.hypothes.is/blog/introducing-developer-api-tokens/