In-Silico Drug Designing Against Ebola Virus: A Genomic Approach
Warwick_Championship presentation
1.
2. Sponsors
ACKNOWLEDGEMENTS:
-Sian Davies (Advisor)
-William Rostain (Advisor)
-Alfonso Rosales-Jaramillo (Supervisor)
-Support of the staff and technicians of Warwick Life Sciences in
particular the mammalian group of David Evans
-Leo Vong
-Chelsey Tye
3. HASSAN (MATHS) IVA (ENGINEERING) CHRIS (PHYSICS) BECKY (BIOMED)
WAQ (BIOLOGY) CARRIE (BIOMED) DAN (MATHS) BEN (MATHS)
17. Split Neomycin Selectivity
Used in selectivity for co-transformants when testing the siRNA and aptazyme in a
separate module to RdRp.
Schmidt, C., Shis, D., Nguyen-Huu, T. and Bennett, M. (2012). Stable Maintenance of
Multiple Plasmids in E. coli Using a Single Selective Marker.
AND LOGIC GATE
Kanamycin
resistance in
prokaryotes
and genticin
resistance in
eukaryotes
18.
19. Testing the Replication system
RNA DEPENDENT RNA POLYMERASE
BL21 E. coli
CELLS
RBS +RdRp
RBS + reverse GFP
+ 3’ RNA Promoter
21. Internal Ribosome Entry Site Testing
EMCV vs NKRF
0
200
400
600
800
1000
1200
1400
1600
1800
EMCV Huh EMCV Hela NKRF Huh NKRF Hela Negative Cntrl
Huh
Negative Cntrl
HeLa
Fluorescence(A.U.) Comparison of efficacy of EMCV and NKRF IRES with controls
22. Aptazyme
IN VITRO FZ Huh no theo
IN VIVO
FZ Huh + theo FZ HeLa no theo FZ HeLa + theo
0mM
4mM
20mM 100mM
26. Further Modelling Developments
The time delay differential equations for our 3’RNA promoter experiment, solved
numerically (in red) and stochastically (in blue, averaging 100 repeats) with the solver
we created
31. Intellectual Property I
If someone modifies an organism or cell such that it now has a new
function, do you believe that the person has right to claim
ownership of this modified organism?
‘No one has the right to life’
‘Modifying cells/organisms in a
particular way is its own unique work
that should be as copyrightable as any
other invention’
YES
48%NO
52%
32. Intellectual Property II
If someone modifies human cells, or maybe even a human, to have
a new/modified function, can that person claim ownership of these
human cells/human?
‘Ownership should only extend to the
copyright over the modified segments of
genetic code. The organism itself, however, is
under it's own ownership if it has sentience.’
‘Technically yes. Obviously you would pay to
have a modification done on you. You're paying
for both a service and a product. The "product"
used to belong them, so technically they were
the previous owners; but once someone buys
them, they are the new owners.’’
YES
13%
NO
87%
33. Associated Dangers
Do you believe Synthetic Biology is a dangerous tool, knowing that
potentially dangerous organisms are being dealt with?
‘Synthetic biology is a double edged sword. It
has equally well advantages and disadvantages.
As responsible scientists/policy makers, we need
to make sure that the technology is not being
used to threaten life.’
YES
30%
NO
45%
OTHER
25%
34. Confidence in SynBio
Would you be happy taking medicine that is derived using bacteria
and viruses which have been selectively modified to serve as a cure
for a particular illness?
YES
94%
NO
6%
35. Bioweapons
Merriam-Webster definition: a harmful biological agent (as a
pathogenic microorganism or a neurotoxin) used as a weapon to
cause death or disease usually on a large scale
Problem 1: using a malicious siRNA
sequence
Problem 2: exploiting self-replication to
promote a hazardous toxin
36. Outreach
Lucie’s testimony: It gave me a new
appreciation for all areas of the research
and the intricacies of carrying it out.’
