NHERF1 regulates the membrane retention and recycling of the parathyroid hormone receptor PTH1R. Specifically:
1) NHERF1 inhibits the endocytosis of PTH1R in response to parathyroid hormone by binding to the receptor via its PDZ domains.
2) This prevents the internalization and delays the recycling of PTH1R after endocytosis.
3) Both the PDZ and MERM domains of NHERF1, as well as the PDZ-binding domain of PTH1R, are required for this effect of reduced endocytosis and delayed recycling.
Introduction to Genetic Variation in GPCR
G-Protein couple Receptor
Genetic variation in GPCRs
V2 Vasopressin Receptor, Thrombroxane Receptor, P2Y 12ADP Receptor, Chemokine Receptor, Biogenic amine receptors
Presented by
R. REKHA
Department of Pharmacology
Introduction to Genetic Variation in GPCR
G-Protein couple Receptor
Genetic variation in GPCRs
V2 Vasopressin Receptor, Thrombroxane Receptor, P2Y 12ADP Receptor, Chemokine Receptor, Biogenic amine receptors
Presented by
R. REKHA
Department of Pharmacology
The slides describe the relation between bile acids, intetsinal microbiota and bile acid activated receptors. Nuclear receptors and G-protein coupled receptors
These are intracellular (cytoplasmic or
nuclear) soluble proteins which respond to
lipid soluble chemical messengers that penetrate
the cell (Fig. 4.10). The receptor protein (specific for each hormone/regulator) is inherently
capable of binding to specific genes, but its
attached proteins HSP-90 and may be some
others prevent it from adopting the configuration needed for binding to DNA. When the
hormone binds near the carboxy terminus of
the receptor, the restricting proteins (HSP-90,
etc.) are released, the receptor dimerizes and
the DNA binding regulatory segment located
in the middle of the molecule folds into the
functionally active configuration. The liganded
receptor dimer moves to the nucleus and binds
other co-activator/co-repressor proteins which
have a modulatory influence on its capacity to
alter gene function. The whole complex then
attaches to specific DNA sequences (hormone
response elements) of the target genes and
facilitates or represses their expression so
that specific mRNA is synthesized/repressed
on the template of the gene. This mRNA
moves to the ribosomes and directs synthesis
of specific proteins which regulate activity of
the target cells.
All steroidal hormones (glucocorticoids,
mineralocorticoids, androgens, estrogens,
progesterone), thyroxine, vit D and vit A function in this manner. Different steroidal hormones affect different target cells and produce
different effects because each one binds to its
own receptor and directs a unique pattern of
synthesis of specific proteins. The specificity as
to which hormone will be bound is provided
by the hormone binding domain, while that as
to which gene will be activated or repressed
is a function of the DNA binding/N-terminus
domain. Different ligands of the same nuclear
receptor have been found to induce ligand-specific
conformations of the receptor so that different
combinations of co-activators and co-repressors
may be bound in different target tissues, e.g.
selective estrogen receptor modulators (SERMs)
tamoxifen and raloxifene have differing patterns
of action on various estrogenic target organs.
Chimeric receptors have also been produced
which respond to one hormone, but produce
the effects of the other hormone.
This transduction mechanism is the slowest in its time course of action (takes hours)
because the adequate quantity of the effector protein
will have to be produced before the response
occurs. The effects also generally out last
the signal (hormone), because the majority of the
generated effector proteins have slow turnover,
and persist in the body even after the hormone
has been eliminated.
Your employees want a bigger piece of the pie. You want to attract and retain top talent while motivating employees to perform at their best. In this webinar, PayScale and BambooHR experts guide you to create a compensation plan that's a win/win for both you and your employees.
The slides describe the relation between bile acids, intetsinal microbiota and bile acid activated receptors. Nuclear receptors and G-protein coupled receptors
These are intracellular (cytoplasmic or
nuclear) soluble proteins which respond to
lipid soluble chemical messengers that penetrate
the cell (Fig. 4.10). The receptor protein (specific for each hormone/regulator) is inherently
capable of binding to specific genes, but its
attached proteins HSP-90 and may be some
others prevent it from adopting the configuration needed for binding to DNA. When the
hormone binds near the carboxy terminus of
the receptor, the restricting proteins (HSP-90,
etc.) are released, the receptor dimerizes and
the DNA binding regulatory segment located
in the middle of the molecule folds into the
functionally active configuration. The liganded
receptor dimer moves to the nucleus and binds
other co-activator/co-repressor proteins which
have a modulatory influence on its capacity to
alter gene function. The whole complex then
attaches to specific DNA sequences (hormone
response elements) of the target genes and
facilitates or represses their expression so
that specific mRNA is synthesized/repressed
on the template of the gene. This mRNA
moves to the ribosomes and directs synthesis
of specific proteins which regulate activity of
the target cells.
All steroidal hormones (glucocorticoids,
mineralocorticoids, androgens, estrogens,
progesterone), thyroxine, vit D and vit A function in this manner. Different steroidal hormones affect different target cells and produce
different effects because each one binds to its
own receptor and directs a unique pattern of
synthesis of specific proteins. The specificity as
to which hormone will be bound is provided
by the hormone binding domain, while that as
to which gene will be activated or repressed
is a function of the DNA binding/N-terminus
domain. Different ligands of the same nuclear
receptor have been found to induce ligand-specific
conformations of the receptor so that different
combinations of co-activators and co-repressors
may be bound in different target tissues, e.g.
selective estrogen receptor modulators (SERMs)
tamoxifen and raloxifene have differing patterns
of action on various estrogenic target organs.
Chimeric receptors have also been produced
which respond to one hormone, but produce
the effects of the other hormone.
This transduction mechanism is the slowest in its time course of action (takes hours)
because the adequate quantity of the effector protein
will have to be produced before the response
occurs. The effects also generally out last
the signal (hormone), because the majority of the
generated effector proteins have slow turnover,
and persist in the body even after the hormone
has been eliminated.
Your employees want a bigger piece of the pie. You want to attract and retain top talent while motivating employees to perform at their best. In this webinar, PayScale and BambooHR experts guide you to create a compensation plan that's a win/win for both you and your employees.
It’s not enough that you drink water every day. You have to make sure it’s the adequate amount and it’s absolutely safe and clean. To be guaranteed about your everyday drinking water, it would be a good idea buy water filter here in Singapore or anywhere you might be in the world.
The latest statistics from WeChat place its monthly active users (MAU) at 700million, with audiences visiting the application upwards of 30 times per day.
While follower numbers for most brands continue to grow, the honeymoon appears to be over. Signs are starting to emerge that follower growth rates for brand accounts are slowing.
At the same time, the government has started to apply pressure to regulate H5 apps built onto WeChat. And Tencent itself is applying greater control over brand activities.
Brands will have to employ more effective content strategies on WeChat moving forward. In this presentation we share our tips to help brands continue to grow by attracting/retaining audiences on WeChat.
The reality for companies that are trying to figure out their blogging or content strategy is that there's a lot of content to write beyond just the "buy now" page.
20 Ideas for your Website Homepage ContentBarry Feldman
Perplexed about what to put on your website home? Every company deals with this tough challenge. The 20 ideas in this presentation should give you a strong starting point.
HIF-1 Gene is transcribed in the nucleus with the help of specific protein .HIF-1 protein with DNA binding activity. Functional HIF transcription factors comprise 2 different subunits, that is, alpha (α) and beta (β). The α subunit, of which there are 3 forms ( HIF-1α, HIF-2α and HIF-3α) out of which HIF-1α and HIF-2α are main, HIF-1α is oxygen sensitive, HIF-1α is expressed in almost all cell types, and transcriptionally upregulates a large number of genes, including those encoding Vascular endothelial growth factor (VEGF), Glucose transporters, Glycolytic pathway enzymes , Insulin-like growth factor-2, Endothelin-1, transferrin, HIF-2 is the primary regulator of EPO production and also plays an important role in enterocyte iron uptake.
HIF-β is continuously transcribed and its mRNA and protein are maintained at constant levels irrespective of oxygen levels, the availability of HIF-α is highly dependent on cellular oxygen levels. Thus, the activity of the HIF transcription factor heterodimer is relatively low under normal tissue oxygen conditions called normaxia however, as cellular oxygen levels decrease called hypoxia, HIF-α concentration increases, making HIF progressively more functionally active.
GPCRs are the most dynamic and most abundant all the receptors. The G protein-coupled receptor (GPCR) superfamily comprises the largest and most diverse group of proteins in mammals. GPCRs are responsible for every aspect of human biology from vision, taste, sense of smell, sympathetic and parasympathetic nervous functions, metabolism, and immune regulation to reproduction. GPCRs interact with a number of ligands ranging from photons, ions, amino acids, odorants, pheromones, eicosanoids, neurotransmitters, peptides, proteins, and hormones.
Nevertheless, for the majority of GPCRs, the identity of their natural ligands is still unknown, hence remain orphan receptors.
The simple dogma that underpins much of our current understanding of GPCRs, namely,
one GPCR gene− one GPCR protein− one functional GPCR− one G protein −one response
is showing distinct signs of wear.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
Honest Reviews of Tim Han LMA Course Program.pptxtimhan337
Personal development courses are widely available today, with each one promising life-changing outcomes. Tim Han’s Life Mastery Achievers (LMA) Course has drawn a lot of interest. In addition to offering my frank assessment of Success Insider’s LMA Course, this piece examines the course’s effects via a variety of Tim Han LMA course reviews and Success Insider comments.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
2. porter (20) and the increase of intracellular calcium (19) in
OK/H cells, which express low levels of NHERF1 and are resist-
ant to the action of PTH. Our previous data indicated that
NHERF1 regulates the conditional efficacy of PTH ligands on
cell-specific PTH1R sequestration (21–23).
Upon binding PTH, as with most GPCRs, the receptor is
phosphorylated by G protein-coupled receptor kinases (24, 25)
and by second messenger-dependent protein kinases (e.g. pro-
tein kinase A and C) (26, 27), and -arrestin is recruited (28, 29).
These processes contribute directly to PTH1R desensitization
by facilitating the uncoupling of the receptor from its cognate G
proteins. The PTH1R undergoes agonist-promoted endocyto-
sis through a clathrin- and dynamin-dependent process (21,
28). Following internalization, the PTH1R is either recycled to
the plasma membrane, leading to receptor resensitization (30)
or degraded through lysosomal and proteosomal pathways,
leading to receptor down-regulation (31, 32).
The effects of NHERF1 on PTH1R retention at the cell mem-
brane and recycling are unknown. In the present study, we
demonstrate that most of receptors are recycled to the cell
membrane after inducing receptor endocytosis with PTH.
Using several different cell models, we show that NHERF1
potently inhibits PTH1R endocytosis and delays PTH1R recy-
cling. This effect requires both intact PDZ and MERM domains
in NHERF1. Likewise, an intact carboxyl-terminal PDZ recog-
nition motif in the PTH1R is also needed.
EXPERIMENTAL PROCEDURES
Materials—HA.11 monoclonal antibody was obtained from
Covance (Berkeley, CA). NHERF1 rabbit polyclonal antibody
was purchased from Affinity Bioreagents (Golden, CO). Horse-
radish peroxidase-conjugated goat anti-rabbit secondary anti-
body was from Pierce. Horseradish peroxidase-conjugated
sheep anti-mouse antibody was from Amersham Biosciences.
Tetracycline hydrochloride was purchased from American Bio-
analytical (Natick, MA). Lipofectamine 2000, zeocin, blastici-
din, and Geneticin were obtained from Invitrogen. Protease
inhibitor mixture Set I and cycloheximide were from Calbio-
chem. Human PTH-(1–34) was purchased from Bachem (Tor-
rance, CA). All other reagents were from Sigma.
Cell Culture—CHO cells (Invitrogen) transfected with
pcDNA6-TR and stably expressing the tetracycline (Tet)
repressor protein were cultured in Ham’s F-12 medium supple-
mented with 10% fetal bovine serum, 100 units/ml penicillin,
100 g/ml streptomycin, and 10 g/ml blasticidin.
MC4 cells were obtained from Dr. G. Xiao (University of
Pittsburgh) and cultured in minimum essential medium (cata-
log number R718-07; Invitrogen) with 10% fetal bovine serum,
100 units/ml penicillin, and 100 g/ml streptomycin.
HEK293 cells were cultured in Dulbecco’s modified Eagle’s
medium/F-12 medium supplemented with 10% fetal bovine
serum, 100 units/ml penicillin, and 100 g/ml streptomycin.
Cells were maintained at 37 °C in a humidified atmosphere of
5% CO2, 95% air.
Construction of pcDNA4/TO-NHERF1, pcDNA3.1ϩ
-HA-
PTH1R, pcDNA3.1ϩ
-HA-M593A, and pcDNA3.1ϩ
-HA-480stop
PTH1R—His-tagged rabbit NHERF1 in pcDNA3.1ϩ
, pcDNA3-
sPDZ1-NHERF1, and pET30A-sPDZ2 were provided by Dr. E. J.
Weinman (University of Maryland). pcDNA3.1ϩ
-His-NHERF1
was cut with KpnI and XhoI, and a 1.1-kb fragment without
epitope was subcloned into the pcDNA4/TO vector (Invitrogen),
whichhastwotetracyclineoperatorsequencesbetweentheTATA
box of the cytomegalovirus promoter and the transcriptional start
site. pET30A-sPDZ2 was cut with KpnI and XhoI and was sub-
cloned into the pCDNA3.1ϩ
vector.
HA-tagged human PTH1R in pcDNA1 (33) (provided by Dr.
T. J. Gardella (Massachusetts General Hospital, Boston) was cut
by HindIII and XbaI and subcloned into the mammalian
expression vector pcDNA3.1ϩ
.
Mutation of the terminal amino acid of HA-PTH1R from
methionine to alanine (M593A) was performed by PCR using
the QuikChange site-directed mutagenesis kit (Stratagene, La
Jolla, CA) according to the manufacturer’s instructions.
HA-PTH1R-480stop was prepared by amplifying the 1–480
sequence by PCR amplified using the forward primer with a
HindIII restriction site, GCG TTT AAA CTT AAG CTT GGT
ACC GAG CTC, and the reverse primer with an XbaI restric-
tion site, GCG GCG TCT AGA TCA TGC CAG TGT CCA
GCG. The purified PCR fragment was cut by HindIII and XbaI
and subcloned into the pcDNA3.1ϩ
.
The fidelity of the plasmids was confirmed by sequencing
(ABI PRISM 377; Applied Biosystems, Foster City, CA) and
subsequent sequence alignment (NCBI BLAST) with rabbit
NHERF1 and human PTH1R (GenBankTM
accession numbers
U19815 and L04308, respectively) to assure the fidelity of the
above constructs.
Stable Expression of pcDNA6-TR, pcDNA4/TO-NHERF1,
and HA-PTH1R—T-REx-CHO cells were transfected with
pcDNA4/TO-NHERF1 or pcDNA/TO vector (control) using
Lipofectamine 2000 following the manufacturer’s instructions
and screening with zeocin (0.4%) and immunoblot. Two cell
lines were obtained. The first, CHO-N10 cells, express
NHERF1 when Tet is added to the cell culture medium. The
other, CHO-EV6, is a control cell line, where NHERF1 cannot
be induced. CHO-N10, CHO-EV6, and HEK293 cells were sta-
bly transfected with pcDNA3.1ϩ
-HA-PTH1R using Lipo-
fectamine 2000 or FuGENE 6 and screened by Geneticin (1.5%)
and immunoblot to generate cell lines (CHO-N10-R3, CHO-
EV6-R4, and HEK293-R25, respectively).
Transient Transfection—Cells, as indicated, were transiently
transfected with 4.0 g of DNA/well in 6-well plates or 1.0 g of
DNA/well in 24-well plates or with empty vector (pcDNA3.1),
plasmids of wild type NHERF1, truncated NHERF1-(1–326)
(NHERF1⌬MERM) (13, 34), mutant NHERF1, in which PDZ1,
PDZ2 or both PDZ1 and PDZ2 domains are scrambled (sPDZ1-
NHERF1, sPDZ2-NHERF1, or sPDZ1/2-NHERF1) (35), wild-
type receptor (HA-PTH1R), mutant receptor (HA-M593A),
truncated receptor (HA-480stop), -arrestin-(319–418), or
HA-K44A dynamin by use of Lipofectamine 2000. Cells were
used 48 h after transfection.
NHERF1 Silencing—Constitutive NHERF1 expression in
HEK293-R25 cells was silenced using RNA interference. Short
hairpin RNA (shRNA) constructs against human NHERF1
sequence GGAAACTGACGAGTTCTTCAAGAAATGCA
mediated by pRS shRNA vector were purchased from OriGene
(TR316855; Rockville, MD). HEK293-R25 cells were trans-
PTH Receptor Recycling
DECEMBER 14, 2007•VOLUME 282•NUMBER 50 JOURNAL OF BIOLOGICAL CHEMISTRY 36215
byguest,onApril6,2011www.jbc.orgDownloadedfrom
3. fected with NHERF1 shRNA or scrambled shRNA, which has
no homology to any human sequence. Transfections were
established following the manufacturer’s protocol. Briefly,
Opti-MEM (Invitrogen) containing 0.5 g of the respective
plasmid and 1.5 l of FuGENE 6 was added to each well of a
24-well plate (about 50% cell confluence). Transfected cells
were cultured for 48 h and then used for receptor recycling or
immunoblot.
Coimmunoprecipitation and Immunoblot Analysis—Inter-
actions of NHERF1 with the indicated PTH1R constructs were
analyzed as described (21). Briefly, 6-well plates of the indicated
cells were transiently transfected with wild-type NHERF1,
sPDZ1-NHERF1, sPDZ2-NHERF1, sPDZ1/2-NHERF1, HA-
PTH1R, M593A-PTH1R, 480s-PTH1R, or the respective
empty vector. Tet (50 ng/ml) was added where indicated. 48 h
later, the cells were lysed with Nonidet P-40 (50 mM Tris, 150
mM NaCl, 5 mM EDTA, 0.5% Nonidet P-40) supplemented with
protease inhibitor mixture I and incubated for 15 min on ice.
Solubilized materials were incubated overnight at 4 °C with
HA.11 monoclonal affinity matrix. Total lysates and immuno-
precipitated protein, eluted by the addition of SDS sample
buffer, were analyzed by SDS-polyacrylamide gels and trans-
ferred to Immobilon-P membranes (Millipore) using the semi-
dry method (Bio-Rad). Membranes were blocked overnight at
4 °C with 5% nonfat dried milk in Tris-buffered saline plus
Tween 20 (TBST) and incubated with different antibodies
(polyclonal anti-NHERF1 antibody at 1:1000 and HA.11 ascites
monoclonal antibody at 1:1000) for 2 h at room temperature.
The membranes were then washed and incubated with goat
anti-rabbit IgG or sheep anti-mouse IgG conjugated to horse-
radish peroxidase at a 1:5000 dilution for 1 h at room temper-
ature. Protein bands were visualized with a luminol-based
enhanced chemiluminescence substrate.
Receptor Binding, Internalization, and Recycling—Receptor
binding, internalization, and recycling assays were performed
as described previously (21, 30) using high pressure liquid
chromatography-purified [125
I][Nle8,18
,Tyr34
]PTH-(1–34)NH2.
PTH1R binding was measured on cells plated on 24-well plates
and grown to confluence. PTH-(1–34) or vehicle was added to
the culture medium and incubated at 37 °C for 30 min. Residual
cell surface binding was removed by rinsing with ice-cold PBS
and acid washing twice with 50 mM glycine, 100 mM NaCl (pH
3). The medium was replenished with Ham’s F-12 containing
10% fetal bovine serum. Cells were put on ice for 15 min and
incubated on ice for an additional 2.5 h with ϳ100,000 cpm of
[125
I][Nle8,18
,Tyr34
]PTH-(1–34)NH2 in 250 l of fresh media.
Nonspecific binding was determined in parallel incubations of
nontransfected CHO-N10 cells with [125
I][Nle8,18
,Tyr34
]PTH-
(1–34)NH2 and was subtracted from total binding to calculate
specific binding or measured in parallel experiments carried
out in the presence of 1 M unlabeled PTH-(1–34) (21, 29).
After incubation, cells were rinsed two times by cold PBS and
then solubilized in 0.2 N NaOH. Cell surface-bound [125
I]PTH-
(1–34) was assessed by ␥ spectrometry. Fractional plasma
membrane PTH1R binding was calculated as ((cpm of unstimu-
lated control Ϫ cpm of PTH)/cpm of unstimulated control) ϫ
100%.
Receptor recycling was determined in a similar manner.
However, after the 30-min PTH treatment and washout, the
cells were returned to 37 °C for the indicated time, after
which PTH binding was measured. PTH1R recycling was
calculated as (cpm of PTH group/cpm of unstimulated
group) ϫ 100%. Receptor number (Bmax) and affinity (Kd)
were measured by competitive binding as described, and
parameters were determined by Scatchard analysis (Prism)
(36).
FIGURE 1. PTH-induced PTH1R internalization. A, time course of PTH1R
internalization in response to 10Ϫ7
M PTH-(1–34). Receptor internalization
was measured as the rate of disappearance of cell membrane binding of
[125
I]PTH-(1–34) as detailed under “Experimental Procedures.” B, effects of
NHERF1 on PTH1R endocytosis and recycling. Plasma membrane PTH1R
abundance was measured in CHO-N10-R3 cells after a 30-min internalization
induced by PTH in the absence or presence of NHERF1. Where indicated, cells
were treated with Tet (50 ng/ml) to induce maximal NHERF1 expression (38).
After ligand washout, cells were allowed to recover for the indicated time.
PTH1Rrecyclingwasmeasuredastherecoveryofmembrane-boundreceptor
binding as described under “Experimental Procedures.” Data are summarized
as the mean Ϯ S.E. of five independent experiments.
PTH Receptor Recycling
36216 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 282•NUMBER 50•DECEMBER 14, 2007
byguest,onApril6,2011www.jbc.orgDownloadedfrom
4. In Vivo Receptor Phosphorylation—In vivo receptor phos-
phorylation was measured as described previously (22) using
[32
P]orthophosphate.
Adenylyl Cyclase Activity—Cyclic AMP accumulation was
determined in subconfluent cells in the presence of the
phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine as
described previously (37).
Statistics—Data are presented as the mean Ϯ S.E., where n
indicates the number of independent experiments. Multiple
comparisons were evaluated by analysis of variance with post-
test repeated measures analyzed by the Bonferroni procedure
(Prism; GraphPad). Differences greater than p Յ 0.05 were
assumed to be significant.
RESULTS
NHERF1 Effects on PTH1R Internalization and Recycling—
Initial characterization of NHERF1 effects on PTH1R traffick-
ing were conducted on an engineered CHO cell model in which
NHERF1 levels could be controlled at a constant and biologi-
cally relevant number of PTH1R. These cells express 6.0 ϫ 105
PTH1R/cell, with an average Kd of 14.2 nM. Tet (50 ng/ml)
induces maximal NHERF1 expression (38).
PTH promoted time- and concentration-dependent PTH1R
internalization. Receptor endocytosis stimulated by PTH began
within 5 min and plateaued by 30 min (Fig. 1A). Therefore, 30
min was used for subsequent determinations of steady-state
and maximal effects. Receptor endocytosis proceeded in a
concentration-dependent manner
with half-maximal PTH1R internal-
ization at 1.2 ϫ 10Ϫ10
M PTH.
Internalized PTH1Rs are either
recycled to the plasma membrane
or degraded (30–32). The influence
of NHERF1 on PTH1R stabilization
and recycling is unknown. In the
absence of NHERF1, a 30-min
challenge with PTH decreased
[125
I]PTH binding by 60%, corre-
sponding to internalization of 40%
of membrane-bound PTH1Rs (Fig.
1B). This degree of PTH1R endocy-
tosis corresponds favorably to that
in other cells and using different
techniques (21, 30, 39). Following
induction of NHERF1 expression,
PTH1R internalization decreased
with a corresponding doubling of
membrane-delimited PTH1R from
40 to 78% (Fig. 1B).
PTH1R recycling was measured
as the rate of recovery of PTH1R
binding following maximal inter-
nalization. Recycling proceeded in a
time-dependent manner with T1⁄2 ϭ
52 min in the absence of NHERF1
and 106 min in the presence of
NHERF1. Virtually complete recy-
cling was achieved by 2 h in either
case. Thus, NHERF1 retards endocytosis and delays PTH1R
recycling.
The addition of Tet to control CHO-EV-R4 cells lacking the
Tet repressor had no effect on receptor internalization or recy-
cling (data not shown). Thus, Tet effects result from induction
of NHERF1 expression and not from toxic or nonspecific
actions. Further, the rate and extent of PTH1R recycling was
indistinguishable in the presence or absence of 200 M cyclo-
heximide (data not shown), suggesting that de novo PTH1R
synthesis does not contribute importantly to the observed
findings.
Similar experiments were performed on MC4 osteoblastic
cells (subclone 4 of MCT3-E1 (40)) to determine if NHERF1
exerts comparable effects in cells constitutively expressing the
PTH1R. MC4 cells express 1.4 ϫ105
PTH1R/cell with an aver-
age Kd of 9.4 nM. After a 30-min exposure to 10Ϫ7
M PTH-(1–
34), 48% of PTH1Rs were located at the cell surface (Fig. 2A).
Receptor recycling proceeded in a time-dependent manner
with complete recycling by 2 h. Endogenous NHERF1 expres-
sion in MC4 cells is negligible (Fig. 2B). Transient transfection
of NHERF1 inhibited PTH-induced internalization without
affecting the rate of receptor recycling.
Complementary experiments were performed on HEK-293
cells, which constitutively express NHERF1 and were stably
transfected with the PTH1R. Here, after a 30-min pretreatment
with 10Ϫ7
M PTH, only 25% of PTH1Rs were internalized, with
75% remaining on the cell surface (Fig. 2C). shRNA targeted to
FIGURE 2. NHERF1 effects on PTH1R tethering and recycling in osteoblasts and HEK-293 cells pre-
treated with PTH. A, MC4 osteoblasts were transiently transfected with vector (control) or wild-type
NHERF1. After 48 h, PTH1R binding and recycling were measured after a 30-min incubation and washout
of PTH. Data are summarized as the mean Ϯ S.E. of three experiments. B, representative immunoblot of
NHERF1 expression in MC4 cells transfected with empty vector or wild-type NHERF1. C, effect of NHERF1
shRNA on cell surface PTH1R binding and recycling. HEK293-R25 cells were transiently transfected with
NHERF1 shRNA or scrambled shRNA (control). After 48 h, the cells were preincubated for 30 min with PTH.
PTH1R binding and recycling were measured as described. Data are summarized as the mean Ϯ S.E. of four
experiments. D, immunoblot showing knockdown of NHERF1 expression by shRNA.
PTH Receptor Recycling
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5. NHERF1 substantially reduced PTH1R binding, consistent
with a role of NHERF1 to stabilize receptors at the plasma
membrane. shRNA reduced endogenous NHERF1 levels by
76% compared with scrambled control (Fig. 2D). The rate con-
stants for recycling (control, 0.03367 minϪ1
; shRNA, 0.02131
minϪ1
) were statistically indistinguishable.
NHERF1 Domains Involved in PTH1R Internalization and
Recycling—NHERF1 possesses two tandem type 1 PDZ
domains. To determine which of these are involved in receptor
recycling, we employed NHERF1 constructs harboring muta-
tions of the core-binding sequences in PDZ-1 (sPDZ1-
NHERF1), PDZ-2 (sPDZ2-NHERF1), or PDZ-1 plus PDZ-2,
(sPDZ1/2-NHERF1). Transient transfection of wild-type
NHERF1 in CHO-EV-R4 cells inhibited receptor internaliza-
tion in response to PTH (Fig. 3A). Interestingly, both sPDZ1-
NHERF1 and sPDZ2-NHERF1 decreased PTH1R internaliza-
tion (Fig. 3A) and interact with the PTH1R (Fig. 3B). However,
double PDZ1 plus PDZ2 mutations (sPDZ1/2) did not affect
PTH1R membrane localization and, as might be expected, did
not associate with the receptor (Fig. 3B). These data show that
interaction of the PTH1R with either PDZ domain is sufficient
to stabilize membrane localization of the PTH1R.
NHERF1 also possess a carboxyl-terminal MERM domain,
linking it with the actin-associated proteins, merlin, ezrin,
radixin, and moesin. We examined the effect of a NHERF1
construct lacking the MERM domain (NHERF1⌬MERM) on
PTH1R internalization and its inter-
action with the PTH1R. As shown in
Fig. 3C, NHERF1⌬MERM had no
effect on PTH1R endocytosis or
recycling despite robust interaction
with the PTH1R (Fig. 3D). Thus, the
effects of NHERF1 on receptor
endocytosis require both intact
PDZ and MERM domains.
PTH1R Determinants of NHERF1
EffectsonInternalization—Thestruc-
tural elements within the PTH1R
involved in the membrane stabi-
lizing effects of NHERF1 and on
receptor recycling were character-
ized. Wild-type PTH1R with an
intact, carboxyl-terminal PDZ rec-
ognition domain (PTH1R-ETVM),
PTH1R with a mutated PDZ recog-
nition motif (PTH1R-ETVA), or a
truncated form of the receptor lack-
ing determinants for stable -arres-
tin association (PTH1R-480stop)
(29) were transiently transfected
into CHO-N10 cells. PTH-stimu-
lated receptor internalization was
60% for both wild-type PTH1R and
PTH1R-ETVA receptors (Fig. 4A).
However, PTH-induced endocyto-
sis was only 35% for the truncated
receptor. These data are consistent
with previous reports implicating
-arrestin in PTH1R internalization (28, 29, 41).
We next determined the effects of NHERF1 on recycling of
the different receptor constructs. NHERF1 did not affect recy-
cling of the truncated or PDZ mutant receptors (Fig. 4A). These
results are compatible with the finding that NHERF1 interacts
only with wild-type PTH1R but not with mutant or truncated
receptors (Fig. 4B) and confirms the requirement for an intact
PDZ recognition motif for the interaction and effects of
NHERF1 on PTH1R stabilization.
NHERF1 Modulates -Arrestin and Dynamin-dependent
Receptor Internalization—PTH translocates -arrestin 2 (21,
28), and PTH1R endocytosis requires dynamin (21). However,
the functional interaction between NHERF1 on -arrestin and
dynamin-dependent PTH1R internalization is unknown. To
investigate the potential involvement of -arrestins in PTH1R
endocytosis, CHO-N10-R3 cells were transiently transfected
with -arrestin 1 or 2. CHO cells exhibit high constitutive levels
of -arrestin expression (42). As expected, overexpression of
-arrestin 1 or -arrestin 2 only minimally increased receptor
internalization (Fig. 5A) compared with control. However,
NHERF1 inhibited receptor internalization in control cells as
well as in cells overexpressing -arrestin 1 or 2. Comparable
effects have been noted in CHO cells stably transfected with the
-opioid receptor and transiently transfected with -arrestin
(43). We reasoned that because CHO cells constitutively
express high levels of -arrestins, dominant negative -arrestin
FIGURE 3. Structural analysis of NHERF1 domains involved in PTH1R trafficking. A, CHO-EV-R4 cells
were transiently transfected with empty vector (control), wild-type NHERF1, or NHERF1 harboring muta-
tions in the PDZ1 and/or PDZ2 core-binding domains (sPDZ1-NHERF1, sPDZ2-NHERF1, or sPDZ1/2-
NHERF1). After 48 h, the cells were preincubated with PTH for 30 min to induce maximal receptor inter-
nalization. PTH1R binding and recycling were measured as before. B, interaction of PTH1R with wild-type
NHERF1, sPDZ1-NHERF1, sPDZ2-NHERF1, or sPDZ1/2-NHERF1. C, membrane tethering of PTH1R requires
an intact NHERF1 MERM domain. Cells were transfected with wild-type or truncated NHERF1 lacking the
MERM domain (NHERF1⌬MERM). PTH1R binding was measured after a 30-min preincubation with PTH.
*, p Ͻ 0.01 versus control. D, representative coimmunoprecipitation experiment showing that PTH1R
interacts with wild-type NHERF1 and NHERF1⌬MERM. IP, immunoprecipitation; IB, immunoblot.
PTH Receptor Recycling
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6. 1-(319–418) (44, 45), which blocks arrestin-clathrin interac-
tions, should inhibit PTH1R endocytosis. Introduction of -ar-
restin 1-(319–418) reduced PTH-stimulated receptor endocy-
tosis by 35% (Fig. 5B). Induction of NHERF1 further decreased
PTH1R internalization by an additional 50% but no greater
than that observed with NHERF1 alone. We also examined the
effect of -arrestin overexpression and inhibition on NHERF1-
sensitive PTH1R recycling. Overexpression of -arrestin 1 or 2
did not affect the magnitude or extent of PTH1R recycling, and
induction of NHERF1 had no greater effect in the presence of
-arrestin 1 or 2 than alone.
Dynamin is a GTPase that regulates the formation of clath-
rin-coated vesicles (46). To determine NHERF1 effects on
dynamin-dependent PTH1R receptor internalization, CHO-
N10-R3 cells were transiently transfected with dominant nega-
tive dynamin (K44A-dynamin). K44A-dynamin inhibited
PTH1R endocytosis (Fig. 5B). The inhibitory effect of K44A-
dynamin was somewhat greater than that of -arrestin-(319–
418). The presence of NHERF1 augmented the inhibitory
action of K44A-dynamin on PTH1R endocytosis by an
additional 50%. These data suggest
that NHERF1 modulates -arres-
tin and dynamin-sensitive PTH1R
internalization.
NHERF1 Does Not Affect PTH1R
Cell Surface Expression, Ligand
Binding, Activation, or Phosphory-
lation—Because NHERF1 regu-
lates PTH1R signaling (18, 20), we
wished to determine if the inhibi-
tory actions of NHERF1 on PTH1R
endocytosis were secondary to an
effect on receptor abundance, li-
gand binding, receptor activation,
or phosphorylation.
Cell surface PTH1R expression
was measured by Scatchard analy-
sis in CHO-N10-R3 cell in the
absence or presence of Tet-in-
duced NHERF1. Bmax was 5.68 ϫ
105
receptors/cell in the absence
of NHERF1 and 6.35 ϫ 105
in the
presence of NHERF1. Kd values
were 10–11 nM in both cases.
As shown in Fig. 6A, [125
I]PTH-
(1–34) binding in the presence and
absence of increasing concentra-
tions of cold PTH (10Ϫ11
to 10Ϫ6
M)
were not altered in the presence of
NHERF1. Thus, NHERF1 does not
inhibit PTH1R endocytosis by
blocking ligand binding to the
PTH1R.
Mahon et al. (18) reported that
NHERF2, a NHERF1 homolog,
markedly inhibited adenylyl cyclase
by stimulating inhibitory Gi pro-
teins in PS120 cells transfected with
the PTH1R. We examined the effect of NHERF1 on PTH-stim-
ulated adenylyl cyclase activity. Neither NHERF1 nor pertussis
toxin affected PTH-stimulated cAMP formation in CHO-
N10-R3 cells (Fig. 6B) or protein kinase A activity (data not
shown).
Finally, we determined the effect of NHERF1 on PTH-stim-
ulated receptor phosphorylation. CHO EV-R4 cells were tran-
siently transfected with HA-tagged NHERF1 and labeled with
[32
P]orthophosphate. PTH-induced receptor phosphorylation
was not enhanced or reduced in the presence of NHERF1 (Fig.
6C). Taken together, these findings show that the effect of
NHERF1 on PTH1R recycling is not due to an alteration of
PTH1R expression, an action on receptor binding, activation,
or phosphorylation.
DISCUSSION
The present studies were initiated to understand better the
regulatory effect of the adapter protein NHERF1 on trafficking
of the PTH1R, a Class B GPCR. NHERF1 is a multifunctional
protein involved in the regulation of signaling and trafficking of
FIGURE 4. PTH1R requires an intact PDZ recognition motif for NHERF1 effects. CHO-N10 cells were tran-
siently transfected with wild-type PTH1R, PTH1R with the PDZ-binding domain mutated to ETVA (PTH1R-
ETVA), or truncated receptor lacking most of the intracellular tail (PTH1R-480stop). A, NHERF1 stabilizes cell
membrane receptors only in cells expressing full-length, wild-type PTH1R with an intact PDZ-binding domain.
Data are summarized as the mean Ϯ S.E. of three experiments. B, representative coimmunoprecipitation
showing that only wild-type PTH1R interacts with NHERF1. HA-tagged receptors were expressed at equivalent
abundance. IP, immunoprecipitation; IB, immunoblot.
PTH Receptor Recycling
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7. GPCRs possessing a requisite PDZ binding domain. In the case
of the PTH1R, for instance, NHERF1 dictates G protein signal-
ing (18), ligand sensitivity or recognition (22), and internaliza-
tion (21). Based on the observations that NHERF1 modulates
recycling of other GPCRs known to interact with NHERF1, we
hypothesized that PTH1R membrane retention and recycling
are regulated by NHERF1. The results establish that NHERF1,
acting both through PDZ domains and the MERM domain,
stabilizes receptor docking at the plasma membrane. However,
recycling of internalized receptors was not appreciably altered.
These findings are qualitatively comparable with those of the
epidermal growth factor receptor, where NHERF1 stabilized
membrane-delimited receptors without affecting the rate of
recycling (14) but contrast with results for -adrenergic and
-opioid receptors, where NHERF1 promoted membrane
retention and increased the rate of recycling (12, 13).
In the case of the 2-adrenergic and -opioid receptors,
NHERF1 promotes rapid recycling to the plasma membrane
(12, 13). ␦-Opioid receptors lack a PDZ-binding domain. How-
ever, the addition of the DSLL PDZ recognition motif from the
2-adrenergic receptor to the ␦-opioid receptor conferred
rapid receptor recycling. These observations provide strong
evidence that NHERF1 accelerates recycling of select Family A
GPCRs. In the case of the PTH1R, a Family B receptor also
possessing a PDZ recognition domain, recycling is inherently
slow and was not substantially affected by NHERF1. Thus, in
this setting, NHERF1 stabilizes the PTH1R at the cell surface
without altering its rate of recycling, which was effectively com-
plete within 2 h in the presence or absence of NHERF1.
A unifying view that may reconcile these divergent observa-
tions is that in the case of the PTH1R (21) and the epidermal
growth factor receptor, a structurally unrelated tyrosine kinase
receptor, NHERF1 interacts constitutively with the receptor,
whereas in the case of 2-adrenergic (12) and -opioid (13)
receptors, interaction with NHERF1 occurs only upon ligand
occupancy of the receptor. Moreover, both 2-adrenergic and
-opioid receptors exhibit rapid dissociation from arrestin and
fast recycling, typical of Class A receptor recycling. These fea-
tures may provide a means for discerning the actions of
NHERF1 on GPCR membrane retention and recycling. Other
aspects of NHERF1 effects may arise from the presence or
absence of additional cis-interacting proteins that account for
the cell-specific actions of NHERF1 on GPCR trafficking.
A novel finding described here is the bifunctional require-
ment for both PDZ and MERM domains in the regulation of
receptor recycling by NHERF1. Wild-type NHERF1 or mutants
harboring a single intact PDZ domain substantially augmented
PTH1R membrane retention. When both PDZ domains were
mutated, the tethering effect of NHERF1 was lost. Consistent
with these functional observations, PTH1R coimmunoprecipi-
tated with intact or single PDZ NHERF mutants but not when
both PDZ core-binding domains were mutated. Thus, PDZ-1
and PDZ-2 NHERF1 domains are operationally redundant in
effecting PTH1R tethering. The NHERF1 MERM domain is
also required for the stabilizing action on PTH1R. When
deleted, NHERF1 lost this effect. Thus, both PDZ and MERM
domains are required for the biological action of NHERF1 on
PTH1R membrane tethering. This conclusion is fortified by
results from the complementary strategy, where the carboxyl-
terminal PDZ-binding domain of the PTH1R was altered.
NHERF1 interacted only with wild-type receptor (PTH1R-
ETVM) to regulate PTH1R membrane retention. NHERF1 had
no effect on the mutant (PTH1R-ETVA) or truncated (PTH1R-
480stop) receptors. Together, these findings strongly support
the view that the effects of NHERF1 on receptor endocytosis
require intact PDZ and MERM domains and require an integral
carboxyl-terminal PDZ recognition motif on the receptor. A
variant of this scheme, where GPCR endocytosis and recycling
were NHERF1-independent but MERM-dependent, has been
described. Stanasila et al. (47) found that ezrin directly binds
␣1b adrenergic receptors, which lack a PDZ recognition
domain, thereby interacting directly with cortical actin without
FIGURE 5. -Arrestin- and dynamin-sensitive NHERF1 inhibition of
PTH1R internalization and recycling. CHO-N10-R3 cells in 24-well plates
were transiently transfected with: A, empty vector, -arrestin 1, or -arrestin
2; B, -arrestin-(319–418) or K44A-dynamin. NHERF1 was induced (Tet, 50
ng/ml) where indicated. After 48 h, the cells were incubated in the presence
or absence of PTH-(1–34) for 30 min. Receptor internalization was assayed as
described in the legend to Fig. 1. Data are summarized as ϮS.E. of three
experiments. **, p Ͻ 0.01 versus PTH-(1–34).
PTH Receptor Recycling
36220 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 282•NUMBER 50•DECEMBER 14, 2007
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8. the involvement of NHERF1 or other intermediary scaffolding
proteins.
Upon ligand binding to the PTH1R, signaling is activated,
and the receptor is phosphorylated by G protein-coupled
receptor kinases (48). We found that NHERF1 did not affect
PTH-(1–34)-stimulated receptor phosphorylation. The PDZ-
binding motif (DSLL) of the 2 adrenergic receptor contains a
serine at the Ϫ2 position that is phosphorylated in response to
receptor activation. Mutation of Ser411
with a phosphomimic
(S411D) or alanine (S411A) blocked interactions with
NHERF1, resulting in targeting of receptors to lysosomes (12).
Thus, in the case of 2-adrenergic receptors, G protein-coupled
receptor kinase-mediated phosphorylation is important for
receptor recycling. The PTH1R lacks an acid residue in the PDZ
recognition domain. This is consistent with the absence of an
effect of NHERF1 on PTH1R phosphorylation and may repre-
sent another level at which receptor-specific regulation of traf-
ficking may be regulated by NHERF1.
The carboxyl terminus of GPCRs is a major regulatory
domain controlling receptor interaction with -arrestins (49).
Truncation of this segment of the PTH1R, for instance, mark-
edly reduced the magnitude and rate of its endocytosis.
NHERF1 further enhanced the inhibitory effects of dominant
negative mutants of arrestin and dynamin. This strongly sug-
gests that the PTH1R is internalized by arrestin- and dynamin-
dependent and -independent processes and that NHERF1
expression affects both mechanisms to similar extents. This
interpretation is consistent with
previously reported observations
that NHERF1 significantly reduces
the lateral diffusion of the recep-
tor (38). Receptor internalization
requires the accumulation of recep-
tors at the site of formation of the
endocytic vesicle. By reducing the
rate of diffusion of the receptor,
NHERF1 therefore slows the accu-
mulation of the PTH1R at these
sites. Interestingly, however, tran-
sient overexpression of -arrestin 1
or -arrestin 2 only minimally
enhanced receptor internalization,
which was still inhibited by
NHERF1. These observations sug-
gest that upon occupancy of the
PTH1R by PTH-(1–34), -arrestins
are engaged and direct the receptor
to endosomes that allow for recep-
tor resensitization and recycling.
These findings confirm a role for
-arrestins in PTH1R internaliza-
tion independent of NHERF1.
In summary, NHERF1 promotes
membrane retention of the PTH1R
in several cell models both endog-
enously and exogenously expressing
NHERF1. The effect of NHERF1 on
receptor endocytosis requires both
intact NHERF1 PDZ and MERM domains. Likewise, an intact
carboxyl-terminal PTH1R PDZ recognition motif is needed.
This action is not due to altered ligand binding, receptor acti-
vation, or phosphorylation. NHERF1 had a negligible effect on
PTH1R recycling. Thus, NHERF1 stabilizes the PTH1R at the
cell membrane and increases the fraction of receptor at the cell
surface. This effect may prevent PTH resistance and PTH1R
down-regulation of PTH1R in cells expressing NHERF1.
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