Monique Denijs (WUR, The Netherlands) Roundtable of aflatoxin experts on “Building a multi-stakeholder approach to mitigate aflatoxin contamination of food and feed” Brussels, Monday 25th January 2016

1. Wageningen UR approach to aflatoxins
One Wageningen
Monique de Nijs, January 2016 Round Table Aflatoxin

2. Wageningen UR
To explore the potential of nature to improve the quality of
life
Wageningen UR is a collaboration between Wageningen
University and the DLO foundation
 6500 staff
 30 locations
 10,000 students
 >2000 PhD students
www.wageningenur.nl
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Crop
Production
System
Climate effects
Predictive modelling
---
Financial & Social impact; Chemical Analysis (on-site); Sampling Strategies
Mycotoxin
production ecology
New products;
rest materials
Food/Feed
Processing
Human nutrition
Allergies
Wageningen UR - aflatoxins
Milk production
systems
EU & National Reference Laboratory; Capacity Building; Legislation
Toxicology
Human &
Animal
Post Harvest
Losses
Diagnostics
Crop protection
management

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RIKILT Wageningen UR
RIKILT contributes to safe and healthy food and animal
feed in the Netherlands and Europe through high-quality
independent research and laboratory & consultancy
services.
Chemical contaminants in food, feed and environment
(mycotoxins, pesticides, dioxins, antibiotics, GMO, ...) in
the supply chain. EU-RL mycotoxins in animal products
 Part of Wageningen UR
 200 staff
Monique de Nijs
www.wageningenur.nl/en/rikilt

6. RIKILT Wageningen UR
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Processes and models
Composition Effects
EURL & NRL
Analytical services
Luminex detection methods
Effect essay trichothecens
Safe Food Chains
Early warning; predictive
modelling mycotoxins
Training & advice
Authenticity (Palm oil)
GMO detection
Allergens
EURL mycotoxins
in food of animal
origin

7.  Targeted quantitative analysis; full-
scan accurate mass for multi-target
screening, biomarker detection and
unknown hazards
 UPLC-ToF / UPLC-FT MS; GC×GC-
MS(MS) Data (magnet sector, triple
quad); GC X GC–TOF-MS; LC-MS(MS)
(triple quad); NMR; FT-IR
 Development and testing of on-site
tests Dipstick / ELISA/LUMINEX
 Chipbased UPLC (NanoTile), green
chemistry
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RIKILT Wageningen UR

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RIKILT Wageningen UR
 Forecasting models
 Sampling strategies

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Trainings unit Food and Feed Analysis:
● Governmental
● Industry
● EURL network
Fit for purpose training:
● Recognised experts
● Proficiency tests
● Laboratory visits / on site training
Capacity Building
RIKILT Wageningen UR

10. RIKILT Wageningen UR
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RIKILT International projects on food safety, a.o.
mycotoxins
 Chile project – Advice Government on National Food
Safety Control System and training future NRLs
 MyToolBox – H2020 – One of the goals develop /
implement biocontrol to reduce aflatoxin contamination
 Selamat – www.selamat.net - International network to
share knowledge, expertise, methodology, best practices
and policy developments related to food production (a.o.
South Africa, Nigeria, Ghana, Mauritius)

11. Plant Research International (PRI)
Wageningen UR
Use the qualities and possibilities of plants to solve
societal issues around food, raw materials and energy
Plants give life
 Part of Wageningen UR
 ~600 staff
 Plant-based raw materials; Healthy foods; Sustainable
production and climate change; Systems biology
www.wageningenur.nl/en/pri
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Biointeractions and Plant Health
Healthy plants to feed the world
Your partner in preventive control measures
and intervention strategies:
 Diagnostics of plant pathogens
 Disease and pest management
 Food safety & phytosanitary issues
 Bees
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PRI Wageningen UR

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Specialized monitoring in the field
• Field sampling strategies
• Processing of sample
• Assay implementation
• Evaluation/decision support
PRI Wageningen UR

14. Available platforms for identification/detection
• ELISA plant dieases
• Colony staining
• LFD/dipsticks
• Real-time PCR
• Luminex
• Next Generation Sequencing
• Isothermal amplification: LAMP
PRI Wageningen UR

15. PRI Wageningen UR
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Collaborations in Africa
 IITA, Nigeria
Aflatoxin control using AflaSafe®
 South Africa (Stellenbosch & ARC)
Mycotoxins/fungi in wheat and maize
 South Africa (Capetown & Eastern Cape)
Mycotoxins and oesophageal cancer

16. PRI Wageningen UR
 MycoKey– H2020 – Reduction of aflatoxin contamination
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What is
required by
users
What is
possible by
technology
What is
viable on the
market
INNOVATION
Wageningen UR

18. Thank you for
your attention
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Wageningen UR
For Quality of Life