VACCINES that provide immunity to the humans

1. Presentation
Topic : Vaccines
NIDHI GOSSAI

2. Introduction
•The word “vaccine” originates from the Latin Variolae vaccinae
(cowpox), which Edward Jenner demonstrated in 1798 could
prevent smallpox in humans.
•Today the term ‘vaccine’ applies to all biological preparations,
produced from living organisms, that enhance immunity against
disease and either prevent (prophylactic vaccines) or, in some
cases, treat disease (therapeutic vaccines).
•Vaccine, suspension of weakened, killed, or fragmented
microorganisms or toxins or of antibodies or lymphocytes that is
administered primarily to prevent disease.
•A vaccine is a biological preparation that provides active
acquired immunity to a particular disease.
•Vaccines are administered in liquid form, either by injection, by
oral, or by intranasal routes.

3. The History Of Vaccination
• The first attempts to prevent disease by using the disease–causing
organism against itself are reported from 7th century India where
Buddhist monks drank snake venom in order to develop immunity
against snake bites.
• Variolation, the practice of inoculating the dried pustules of
smallpox (caused by the Variolae virus) from a sick individual into a
healthy individual, to prevent the healthy individual from
developing the disease, developed in Central Asia in the second
millennium. The practice then spread east to China and West to
Turkey, Africa, and Europe.
• In 1798, in England, Edward Jenner published the results of his
experiments on “vaccination”, the practice of inoculating the
cowpox virus (closely related to the human smallpox virus), Variolae
vaccinae, to prevent smallpox in humans. The term vaccination was
derived from vaccinae virus. The practice became widely
popularized.

4. The history of vaccination contd….
• At the end of the 19th century, Louis Pasteur began to apply the concept
of vaccination to other diseases. He demonstrated that the harmful nature
of disease-causing organisms could be weakened (or attenuated) in the
laboratory. He first demonstrated the effectiveness of vaccines against
chicken cholera and anthrax in animals, before developing his vaccine
against rabies for use in humans in 1885.
• In 1886, in the US, Daniel Elmer Salmon and Theobald Smith
demonstrated that vaccines could be produced not just from live
organisms, but also from killed disease-causing organisms. Their discovery
would lead to the subsequent development of inactivated vaccines against
several human diseases.
• In the early 20th century, it was discovered that some diseases were
caused not by bacteria themselves, but by the toxins that they produced.
Inactivated toxins acted like vaccines by providing protection against these
toxininduced diseases. These vaccines are known as toxoids.
• By the end of the 20th century, a spurt of innovation led to the
development of several new methods of producing vaccines including by
recombinant organisms, by conjugation of polysaccharides to carrier
proteins, and by the assembly of virus-like particles.

6. Types of Vaccines
• Attenuated :From living organisms that have been weakened,
usually from cultivation under sub-optimal conditions (also called
attenuation), or from genetic modification, which has the effect of
reducing their ability to cause disease.
• Inactivated : From whole organisms that have been inactivated by
chemical, thermal or other means.
• Recombinant subunit : From components of the disease-causing
organism, such as specific proteins and polysaccharides, or nucleic
acids.
• Toxoid : From inactivated toxins of toxin-producing bacteria.
• Conjugate polysaccharide- protein : From the linkage (conjugation)
of polysaccharides to proteins (this increases the effectiveness of
polysaccharide vaccines in young children) .

7. Construction of vaccines

8. TABLE 1. EXAMPLES OF VACCINES BY TYPE

9. Vaccine combinations
• In addition to combining several serotypes of a disease causing
organism in a single vaccine, vaccines against different disease-
causing organisms can be combined to provide protection against
several different diseases.
• These combination vaccines may contain different types of
vaccines.
• Combination vaccines against different diseases such as diphtheria,
tetanus, pertussis, Heamophilus influenzae type b, Hepatitis B, and
polio, are commonly used in childhood immunization schedules.
• These vaccines incorporate both viral and bacterial vaccines and
contain toxoids, purified protein sub-unit vaccine, conjugated
polysaccharide vaccine, recombinant protein vaccine, and
inactivated viral vaccine respectively.

10. What does a vaccine contain?
• Vaccines may also contain antigens against several types (or serotypes) of
the same disease-causing organism, providing protection against each
type.
• For example, Polio and influenza vaccines each protect against 3 types of
virus, and some bacterial vaccines like pneumococcal vaccine protect
against up to 23 different serotypes of Streptococcus pneumoniae.
• In addition to the bulk antigen that goes into a vaccine, vaccines are
formulated (mixed) with other fluids (such as water or saline), additives or
preservatives, and sometimes adjuvants.
• Collectively, these ingredients are known as the excipients. These ensure
the quality and potency of the vaccine over its shelf-life.
• Vaccines are always formulated so as to be both safe and immunogenic
when injected into humans. Vaccines are usually formulated as liquids, but
may be freeze-dried (lyophilized) for reconstitution immediately prior to
the time of injection.

11. Preservatives
• Preservatives ensure the sterility of the vaccine over
the period of its shelf-life. Preservatives may be used to
prevent contamination of multi-dose containers.
• Or, in some cases, preservatives may be added during
manufacture to prevent microbial contamination.
• Preservatives used in vaccines are non-toxic in the
amounts used and do not diminish the potency of
vaccines.
• But not all preservatives can be used in all vaccines.
Some preservatives will alter the nature of some
vaccine antigens.

12. EXAMPLES OF VACCINES WITH
PRESERVATIVES
Preservative
Phenol
Benzithonium Chloride
2- phenoxyethanol
Thimerosal
Vaccines
Typhoid, pneumococcol
polysaccharide
Anthrax
Inactivated polio
Multi-dose influenza

13. Adjuvants
• In addition to preservatives, some vaccines
contain adjuvants.
• Adjuvants enhance the immune effect of the
vaccine antigen, but do not themselves act as
antigens.
• Aluminum salts are the most commonly used
adjuvant for vaccines.
• Adjuvanted vaccines may have a slightly higher
rate of adverse reactions, including pain at the
injection site, malaise and fever

14. EXAMPLES OF ADJUVANTED VACC
Adjuvanted Vaccine
Hepatitis A
Hepatitis B
Diphtheria, Tetanus, acellular
Pertussis combinations (DTaP
or Tdap)
Haemophilus influenzae type
b (Hib)
Human Papilloma Virus (HPV)
Pneumococcal conjugate
Japanese encephalitis
H1N1 influenza
Type of Adjuvant
Aluminum salt
Aluminum salt
Aluminum salt
Aluminum salt
Aluminum salt or AS04
(aluminum salt and
monophospholipid A)
Aluminum salt
Aluminum salt
MF59 (oil in water emulsion)
[one vaccine]

15. How do vaccines work?
• When inactivated or weakened disease-
causing microorganisms enter the body, they
initiate an immune response.
• This response mimics the body’s natural
response to infection.
• But unlike disease-causing organisms,
vaccines are made of components that have
limited ability, or are completely unable, to
cause disease

16. How do vaccines work? Contd..
• The components of the disease-causing
organisms or the vaccine components that
trigger the immune response are known as
“antigens”.
• These antigens trigger the production of
“antibodies” by the immune system.
• Antibodies bind to corresponding antigens
and induce their destruction by other immune
cells

18. How do vaccines work?Contd…
• The induced immune response to either a disease-causing
organism or to a vaccine configures the body’s immune
cells to be capable of quickly recognizing, reacting to, and
subduing the relevant disease-causing organism.
• When the body’s immune system is subsequently exposed
to a same disease-causing organism, the immune system
will contain and eliminate the infection before it can cause
harm to the body.
• Some disease-causing organisms, such as influenza, change
from year to year, requiring annual immunization against
new circulating strains. In very young children, the immune
system is immature and less capable of developing
memory. In this age group, duration of protection can be
very short-lived for polysaccharide antigens.

20. Don’t wait-Vaccinate !
Thank you…