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Ultraviolet & flourescence spectroscopy instrumentation
This document discusses UV-VIS and fluorescence instrumentation. It explores UV-VIS instrumentation, including single beam and double beam UV-VIS spectrophotometers. It also discusses fluorescence instrumentation and the components of a fluorescence spectrophotometer, including the light source, excitation and emission wavelength selectors, sample holder, and transducer for detecting emission. The document also briefly mentions phosphorescence and different types of luminescence.
Ultraviolet & flourescence spectroscopy instrumentation
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- 2. WE ARE GOINGTO EXPLORE: UV-VIS Instrumentation aspects of FLOURESCENCE PHOSPHORESCENCE
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- 4. Single beam UV-VISspectrophotometer Double beam UV-VIS spectrophotometer chem .iit bombay Indiamart.com
- 5. UV-VIS INSTRUMENTATION LIGHT SOURCEMIRRORSMONOCHROMATORBEAM SPLITTER* SAMPLECELLS / CUVETTES DETECTORAS SIMPLE AS THAT
- 6. Jd.com Tuopeek.com Tunsten halogenlamp Deuterium discharge lamp
- 7. An ideal monochromatershould possess: 1)high peak transmittance 2)low band pass absorption filters Filters interference filters refractive type prisms reflective type Gratings are preferred over prisms because they are resistant to moisture and also because of their low band pass of 0.1nm. Also there is no energy loss due to absorption in gratings.
- 8. CAN U HELP MEREACH THE DETECTOR?
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- 10. DOUBLE BEAM –INSPACE SPECTROPHOTOMETER mientayvn.com
- 11. DOUBLE BEAM –INTIME SPECTROPHOTOMETER Chemguide.in
- 12. Differences b/w singleand double beam spectrophotometers: 1) Number of beams 2) Reliable absorbance values 3) High quality components • Handling voltage fluctuations • Handling time dependent irregularities 4) Complexity of instrument 5) Speed
- 13. APPLICATIONS • In calculationof Molar absorptivity (Ꜫ) of a substance • Detection of functional groups • In Dye degradation studies • Detemination of unknown concentration using calibration plot • In Ion sensing studies ( e.g. fluoride sensing)
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- 19. “TEST IS MYTASTE” says