3. Transgenic animals
Why?
Selective breeding is performed
centuries.
Breeding is time consuming.
• since
•
•
•
Crossing in properties is time consuming.
Only a limited number of properties
available.
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4. Transgenic animals
For what reason?
o Introduction of a desired property
o Fast generation of animal lines carrying desired property
o Animal model for human diseases
o Animal system to produce biomolecules
o Xeno-Transplantates
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6. Transgenesis in Mice
Retroviral Vector
•
•
•
•
Efficient mechanism of transgene
Transfer of genes < 8 kb possible
random insertion of the transgene
integration
Retroviral contamination of the transgenic
animal
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7. Transgenes in mice
Microinjection
• Low efficient mechanism of transgene
integration (5-10%)
Transfer of large genes possible
random insertion of the transgene
Variable expression levels
no selection of the transgene necessary
•
•
•
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19. First clone cat•
• Derived from
fibroblast cell by
nucleus transfer.
• Coat color differs
between the
genetic mother
and the offspring
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