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An overview of agricultural applications of genome editing: Farm animals

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The presentation will give an overview of genome editing applications in relation to farm animals. It will give an overview of some examples of agricultural applications that may be on or close to the market or under research and development. It will also consider the possibility of foreseeing future applications (e.g. variations in CRISPR/Cas applications, DNA-free
application, agricultural pest control), if possible.

Published in: Environment
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An overview of agricultural applications of genome editing: Farm animals

  1. 1. Simon Lillico Agricultural applications of genome editing: farmed animals
  2. 2. Why modify livestock?
  3. 3. DNA sequencing
  4. 4. Breeding pyramid Pedigree stock GGP Grandparent Parent Production
  5. 5. GGP Grandparent Parent Production Pedigree stock Breeding pyramid Pedigree stock Pedigree stock
  6. 6. We are now in the era of re-writing the genome
  7. 7. What does genome editing bring to the table? • Conventional breeding – Generation time is long, so progress is slow – Restricted to existing traits – Difficult to introgress new traits into elite stock • Editors – Generation time is still long… … cell based approaches allow rapid trait introgression – Traits can be introgressed into elite stock without genetic dilution – Multiple edits introduced concurrently – New traits can be “designed” Trait X
  8. 8. Multiple applications of genome editing New Scientist
  9. 9. Various routes to genome edited livestock
  10. 10. Avian primordial germ cells Macdonald et al., 2010
  11. 11. Fish NOAA Fisheries
  12. 12. Mammals Park et al., 2017 Spermatagonial stem cells CNIC Cloning or direct manipulation
  13. 13. CNIC Mammalian zygotes
  14. 14. CNIC Mammalian zygotes
  15. 15. CNIC Mammalian zygotes
  16. 16. CNIC Mammalian zygotes
  17. 17. Embryo transfer
  18. 18. What sort of changes can we engineer? • Production traits – monogenic or polygenic • Environmental adaptation • Disease resistance • Welfare
  19. 19. African swine fever
  20. 20. Moving genetic variation across species barriers T448A S485P S531P RHD TADNH2 COOH Transfer allelic variation between species
  21. 21. 1.5B € annually Vaccines not cross-protective HIPRA Porcine Reproductive and Respiratory Syndrome
  22. 22. PRRSV CD163 gene Designing novel traits
  23. 23. CD163 gene PRRSV Designing novel traits
  24. 24. CD163 gene PRRSV Designing novel traits
  25. 25. Wild Type Heterozygous Homozygous WT Het Hom Edited pigs
  26. 26. PRRSV-resistant pigs FDA
  27. 27. What’s next for editing • Cas9-like enzymes from new species (toolbox) • Base editors • Targeted RNA degradation • Transcriptomic modifiers

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