The TNAU Seed Portal is developed to provide a comprehensive and user-friendly database on seed standards, varietal characteristics, and quality certification, utilizing SSR marker fingerprinting to ascertain seed purity and classification. It includes a three-layer architecture with an HTML/CSS user interface, PHP as an intermediary, and MySQL for data storage, facilitating dynamic updates. Future developments aim to enhance accessibility through mobile applications and expand the SSR marker data available.

1. TNAU Seed Portal
A.Dineshwaran BTI-09-030
TNAU Genomics – http://www.tnaugenomics.com/seedportal

2. Introduction
• Seed play an important role in any technology embedding
and we have to produce pure, quality, resistant, healthy
seeds for production of healthy food to satisfy our needs.
• The vast variety of information regarding seeds is scattered
and no such organized one stop database available at present
• Hence information regarding quality seed production,
maintenance of seed quality and purity, minimum seed
standards of the crop, Varietal characters, DUS characters and
many are incorporated in the user friendly interface.
• To provide the search based on SSR Marker fingerprinting in
ten major cultivated paddy varieties in TamilNadu.
TNAU Genomics – http://www.tnaugenomics.com/seedportal

3. Review on Seed Certification
• Available Seed Certification Database such as SeedNet India,
(www.seednet.gov.in) and Database provided by TamilNadu
Seed Department, (http://www.seedtamilnadu.com/)
provides the details on Indian Minimum Seed Certification
Standards and Quality Control.
• These databases are not equipped with an user-friendly
search option to enable the users to access the information in
a easier manner.
• With a view to provide a user friendly interface for users,
TNAU Seed Portal has been developed.
TNAU Genomics – http://www.tnaugenomics.com/seedportal

4. Varietal Identification
• There are many varieties of crop
available.
• Growers take advantage of these
differences and choose the specific
varieties that best suit the
requirements and conditions of their
farm and the market.
• Market forces are increasingly
demanding pure (or single) varieties of
grains for specific purposes.
• This means varietal testing must
become a standard practice in order to
guarantee the sale of grain.
TNAU Genomics – http://www.tnaugenomics.com/seedportal

5. Marker Fingerprint Identification
• Conventionally, morphological
descriptors are routinely used
for establishing the identities
of varieties.
• But these morphological
descriptors suffer from many
drawbacks such as influence
of environment on trait
expression.
• To overcome these defects ,
Marker Fingerprint
Identification has been
adopted.
TNAU Genomics – http://www.tnaugenomics.com/seedportal

6. SSR Marker Fingerprinting
• The Morphological and DUS Characters of 62 varieties has already
been integrated in the database.
• In a view to distinguish the varieties on the molecular basis level SSR
marker fingerprinting has been done.
( Ms.Vigneshwari , Genomics Lab, CPMB).
• DNA was extracted from ten major varieties cultivated in TamilNadu
such as ADT37, ADT43, ADT46, ASD16, Bhavani, CO43,
CO48,CO49,CO50, CR1009, IR20, IR50 and White Ponni.
• The SSR Primers used are RM5638, RM3515, RM231, RM252,
RM163, RM225, RM6728, RM8226, RM219, RM228, RM144 and
RM511.
• The PCR products are run on agarose gel and then result is
documented.
• For the easy access to the documented data , an database has been
developed which can be updated.
TNAU Genomics – http://www.tnaugenomics.com/seedportal

7. Database Architecture
• Three layers
– Web page-User Interface
– Interface-Intermediating Platform
– Database-Data Storage
• User Interface is designed using HTML/CSS.
• PHP (Hypertext Pre Processor) acts as an Intermediary
Platform.
• MySQL database serves as an data storage server.
• PHP is an server side scripting language which enables us to
maintain an dynamic database.
TNAU Genomics – http://www.tnaugenomics.com/seedportal

8. Database Schema
HTML
PHP
MYSQL
TNAU Genomics – http://www.tnaugenomics.com/seedportal

9. Page View – Search Page
TNAU Genomics – http://www.tnaugenomics.com/seedportal

10. Page View- Result
TNAU Genomics – http://www.tnaugenomics.com/seedportal

11. Page View – Marker data page
TNAU Genomics – http://www.tnaugenomics.com/seedportal

12. Future Development
• TNAU Seed Portal is an is an organized database that provides the
information about all crops seed standards, Varietal characters, DUS
characters seed production, processing, testing techniques and
seed legislations.
• Since, SSR Marker Fingerprinting data enables the researchers to
classify the various types of cultivars in a efficient way. It can lead to
seed purity.
• Due to recent developments in IT, steps has been taken to make the
database available to the users in the form of Android application
so that data can be accessed using mobile phones.
• The dynamic nature of the PHP enables us for the further updates
in marker data.
• More SSR markers for each chromosome of paddy will be added in
the days to come.
TNAU Genomics – http://www.tnaugenomics.com/seedportal

13. Reference
• Tunwar N.S. ,Singh S.V .Indian Minimum Seed Certification
Standards ;The central seed certification board July 1988.
• Mario Latendresse and Peter D. Karp (2010). An advanced
web query interface for biological databases. Database, Vol.
2010.
• Brad Bulger, Jay Greenspan and, David Wall (2004).
MySQL/PHP Applications. Wiley Publications.
• http://www.php.net/
• http://www.tnaugenomics.com/duster/
• http://www.w3schools.com/
TNAU Genomics – http://www.tnaugenomics.com/seedportal