The document summarizes research on the effects of ocean acidification on Pacific oyster larval development and physiology. Key findings include:
1) Higher CO2 levels (lower pH) delayed larval development and reduced the proportion of larvae that had started calcifying at 24 hours.
2) Larvae reared at higher CO2 were smaller, as measured by hinge length and shell height, indicating reduced growth and calcification.
3) By 72 hours, a lower proportion of larvae had fully calcified shells under the highest CO2 treatment, suggesting acidification impairs the calcification process.
A copy of the presentation I gave at the AusIMM 2009 International Uranium Conference. It outlines some testing I conducted on a Brannerite ore to beneficiate it.
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Smart TV Buyer Insights Survey 2024 by 91mobiles.pdf91mobiles
91mobiles recently conducted a Smart TV Buyer Insights Survey in which we asked over 3,000 respondents about the TV they own, aspects they look at on a new TV, and their TV buying preferences.
UiPath Test Automation using UiPath Test Suite series, part 4DianaGray10
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The UiPath Test Manager overview with SAP heatmap webinar offers a concise yet comprehensive exploration of the role of a Test Manager within SAP environments, coupled with the utilization of heatmaps for effective testing strategies.
Participants will gain insights into the responsibilities, challenges, and best practices associated with test management in SAP projects. Additionally, the webinar delves into the significance of heatmaps as a visual aid for identifying testing priorities, areas of risk, and resource allocation within SAP landscapes. Through this session, attendees can expect to enhance their understanding of test management principles while learning practical approaches to optimize testing processes in SAP environments using heatmap visualization techniques
What will you get from this session?
1. Insights into SAP testing best practices
2. Heatmap utilization for testing
3. Optimization of testing processes
4. Demo
Topics covered:
Execution from the test manager
Orchestrator execution result
Defect reporting
SAP heatmap example with demo
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Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
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This video focuses on the notifications, alerts, and approval requests using Slack for Bonterra Impact Management. The solutions covered in this webinar can also be deployed for Microsoft Teams.
Interested in deploying notification automations for Bonterra Impact Management? Contact us at sales@sidekicksolutionsllc.com to discuss next steps.
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State of global ICS asset and network exposure
Sectoral targets and attacks as well as the cost of ransom
Global APT activity, AI usage, actor and tactic profiles, and implications
Rise in volumes of AI-powered cyberattacks
Major cyber events in 2024
Malware and malicious payload trends
Cyberattack types and targets
Vulnerability exploit attempts on CVEs
Attacks on counties – USA
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In-depth analysis of the cyber threat landscape across North America, South America, Europe, APAC, and the Middle East
Why are attacks on smart factories rising?
Cyber risk predictions
Axis of attacks – Europe
Systemic attacks in the Middle East
Download the full report from here:
https://sectrio.com/resources/ot-threat-landscape-reports/sectrio-releases-ot-ics-and-iot-security-threat-landscape-report-2024/
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All these questions and more will be explored as we talk about matching clients’ needs with what your agency offers without pulling teeth or pulling your hair out. Practical tips, and strategies for successful relationship building that leads to closing the deal.
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The key trends across hardware, cloud and open-source; exploring how these areas are likely to mature and develop over the short and long-term, and then considering how organisations can position themselves to adapt and thrive.
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LF Energy Webinar: Electrical Grid Modelling and Simulation Through PowSyBl -...DanBrown980551
Do you want to learn how to model and simulate an electrical network from scratch in under an hour?
Then welcome to this PowSyBl workshop, hosted by Rte, the French Transmission System Operator (TSO)!
During the webinar, you will discover the PowSyBl ecosystem as well as handle and study an electrical network through an interactive Python notebook.
PowSyBl is an open source project hosted by LF Energy, which offers a comprehensive set of features for electrical grid modelling and simulation. Among other advanced features, PowSyBl provides:
- A fully editable and extendable library for grid component modelling;
- Visualization tools to display your network;
- Grid simulation tools, such as power flows, security analyses (with or without remedial actions) and sensitivity analyses;
The framework is mostly written in Java, with a Python binding so that Python developers can access PowSyBl functionalities as well.
What you will learn during the webinar:
- For beginners: discover PowSyBl's functionalities through a quick general presentation and the notebook, without needing any expert coding skills;
- For advanced developers: master the skills to efficiently apply PowSyBl functionalities to your real-world scenarios.
Dev Dives: Train smarter, not harder – active learning and UiPath LLMs for do...UiPathCommunity
💥 Speed, accuracy, and scaling – discover the superpowers of GenAI in action with UiPath Document Understanding and Communications Mining™:
See how to accelerate model training and optimize model performance with active learning
Learn about the latest enhancements to out-of-the-box document processing – with little to no training required
Get an exclusive demo of the new family of UiPath LLMs – GenAI models specialized for processing different types of documents and messages
This is a hands-on session specifically designed for automation developers and AI enthusiasts seeking to enhance their knowledge in leveraging the latest intelligent document processing capabilities offered by UiPath.
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Watch this recorded webinar about real-time monitoring of application performance. See how to integrate Apache JMeter, the open-source leader in performance testing, with InfluxDB, the open-source time-series database, and Grafana, the open-source analytics and visualization application.
In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
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During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
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https://www.rttsweb.com/jmeter-integration-webinar
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All of this illustrated with link prediction over knowledge graphs, but the argument is general.
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Send an interactive Slack channel message (using buttons)
Have the message received by managers and peers along with a test email for review
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In a second workflow supporting the same use case, you’ll see:
Your campaign sent to target colleagues for approval
If the “Approve” button is clicked, a Jira/Zendesk ticket is created for the marketing design team
But—if the “Reject” button is pushed, colleagues will be alerted via Slack message
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And...
Speakers:
Akshay Agnihotri, Product Manager
Charlie Greenberg, Host
Connector Corner: Automate dynamic content and events by pushing a button
Timmins schiffman pcsga 2011
1. The Effects of Ocean Acidification on
Pacific Oyster Larval Development
and Physiology
Emma Timmins-Schiffman
Steven Roberts
Carolyn Friedman
Michael O’Donnell
University of Washington
PCSGA
Salem, OR, September, 2011
2. How does OA affect larvae?
Effect of OA Organism Reference
Decreased shell Oyster, mussel, 1, 2, 3, 4, 9, 12
size, strength, barnacle, crab
calcification
Transcriptome/ Urchin 5, 6, 10
physiology
Protein Barnacle 7
Developmental Urchin, shrimp, 8, 9, 13
delay and change brittle star
in energy budget
Increased growth Sea star 11
rate
Abnormal Brittle star, urchin, 12, 2
morphology oyster
Response to other Urchin, barnacle, 14, 3
stressors crab
3. Which physiological mechanisms are
changing?
¤ Calcification
¤ Hydrogen ion balance across membranes
¤ Energy metabolism
¤ Timing of developmental processes
¤ Stress response
4. How does ocean acidification affect
development and physiology of Pacific oyster
larvae (Crassostrea gigas)
5. CO2-free air
CO2 (canister)
Honeywell Controller
Treatment-
equilibrated
water
DuraFET pH probe Venturi injector
6. Experimental Design
Equilibrate treatment water
Fertilization 1 hpf 6 hpf 24 hpf 72 hpf 96 hpf
Fix samples for
Sample for transcriptomics
developmental stage, size,
and calcification
11. Results: Larval Development, Growth,
and Calcification
¤ Larvae were fixed for later microscopy
¤ Developmental stage was assessed
¤ Growth was measured: hinge length, shell
height
¤ Calcification: double polarization of light
12. Proportion Larvae at Pre-Hatching Stage at 6hpf
1.0
400 !atm
700 !atm
1000 !atm
0.8
Proportion at Pre-Hatching
Significantly
0.6
fewer larvae are
in an advanced
0.4
developmental
stage at higher
pCO2 (lower pH)
0.2
0.0
400 700 1000
Treatment
14. Larval Calcification at 24h
More larvae
1.0
400 !atm
700 !atm have started
1000 !atm calcification
0.8
at higher
Proportion Calcified
pCO2
0.6
0.4
0.2
0.0
400 700 1000
Treatment
15. Larval Calcification at 72h
Fewer larvae
1.0
are fully
calcified in
the highest
0.8
pCO2
Proportion Calcified
(lowest pH)
0.6
treatment
0.4
400 !atm
0.2
700 !atm
1000 !atm
0.0
400 700 1000
Treatment
16. Larval Size: Methods
¤ Size measured in 2
parameters – hinge
length and shell
height
¤ Measurements are
from 24 and 72
hours post
fertilization
17. Hinge Length by Treatment and Day Shell Height by Treatment and Day
80
70
70
60
Hinge Length (!m)
Shell Height (!m)
50
60
40
50
30
40
D1 400 D1 700 D1 1000 D3 400 D3 700 D3 1000
D1 400 D1 700 D1 1000 D3 400 D3 700 D3 1000
Day and pCO2 (!atm)
Day and pCO2 (!atm)
Larvae are smaller at higher pCO2 at 3
days post-fertilization
18. Growth Rate by Treatment
15
Hinge
Height
Shell height
Growth Rate/Day (!m)
10
growth rate is
slower at the
highest pCO2
5
0
400 700 1000
Treatment (!atm)
19. Gene Expression
¤ 2 microcosms from
each treatment at
96 hpf
¤ Oxidative stress
genes (SOD,Prx6)
and molecular
chaperone (Hsp70)
20. Hsp70
Stress Response
STRESS Protein damage/
unfolding
Hsp70
Chaperones bind to proteins to either repair or remove
21. Heat Shock Protein 70
25
Fold Over Minimum Expression
Increased
20
expression
of hsp70
with
15
increased
pCO2 could
indicate
10
cellular
stress
5
400 700 1000
Treatment (!atm)
25. Oxidative Stress Genes
Greater expression of SOD and Prx6 may
indicate increased oxidative stress during
exposure to ocean acidification
26. Conclusions
¤ pCO2 of 700 and 1000 µatm caused decreased growth
and calcification in C .gigas larvae through 72 hpf
¤ There is evidence of physiological stress
¤ Significant for exposure to other stressors
¤ Significant for continued growth, development, and survival
27. Thank you
Emily Carrington•Matt George•Michelle Herko•Laura
Newcomb•Ken Sebens•Richard Strathmann•Adam
Summers•Billie Swalla•Brent Vadopalas
Chelsea Farms LLC
Little Skookum Shellfish Growers
Rock Point Oyster Co.
Seattle Shellfish
Taylor Shellfish
NOAA Aquaculture
NSA, Pacific Coast Section
28. References
¤ 1Watson et al. 2009. Early larval development of the Sydney rock oyster Saccostrea glomerata under near-future predictions of CO2-driven ocean acification. Journal of Shellfish Research. 28
(3)): 431-437.
¤ 2 Gaylord et al. 2011 Functional impacts of ocean acidification in an ecologically critical foundation species. J Exp Biol. 214: 2586-2594.
¤ 3 Parker et al. 2010. Comparing the effect of elevated pCO2 and temperature on the fertilization and early development of 2 species of oyster. Marine Biology. 157(11): 2435-2452.
¤ 4 Findlay et al. 2009. Post-larval development of 2 intertidal barnacles at elevated CO2 and temperature. Mar Biol. 157: 725-735.
¤ 5 Todham & Hofmann 2009 Transcriptomic response of sea urchin larvae Strongylocentrotus purpuratus to CO2-driven seawater acidification. J Exp Biol. 212: 2579-2594.
¤ 6 Stumpp et all. 2011. CO2 induced seawater acidification impacts sea urchin larval development II: Gene expression patterns in pluteus larvae. Comparative Biochemistry and Physiology – Part
A. 160(3): 320-330.
¤ 7 Wong et al. 2011. Response of larval barnacle proteome to CO2-driven seawater acidification. Comparative Biochemistry and Physiology – Part D. 6(3): 310-321.
¤ 8 Stumpp et al. 2011. CO2 induced seawater acidification impacts sea urchin larval development I: Elevated metabolic rates decrease scope for growth and induce developmental delay.
Comparative Biochemistry and Physiology – Part A. 160(3): 331-340.
¤ 9 Bechmann et al 2011. Effects of ocean aciidification on early life stages of shrimp (Pandalus borealis) and mussel (Mytilus edulis). J Toxicol Environ Health. 74(7-9): 424-438.
¤ 10 Martin et al. 2011. Early development andmolecular plasticity in the Mediterranean sea urchin Paracentrotus lividus exposed to CO2-driven aciidification. J Exp Biol. 214(8): 1357-1368.
¤ 11 DuPont et al. 2010. Near future ocean acidification increases growth of lecithotrophic larvae and juveniles of the sea star Crossaster papposus. J Exp Biol Part B. 314B(5): 382-389.
¤ 12 Kurihara et al. 2007. Effects of increased seawater pCO2 on early development of the oyster C.rassostrea gigas. Aquat Biol. 1:91-98.
¤ 13 Dupont et al. 2008. Near-future level of CO2-driven ocean acidification radically affects larval survival and development in the brittlestar Ophiothrix fragilis. Mar Ecol Prog Ser. 373: 285-294.
¤ 14 O’Donnell et al. 2009. Predicted impact of ocean acidification on marine invertebrate larvae: elevated CO2 alters response to thermal stress in sea urchin larvae. 156(3): 439-446.