This document summarizes experiments testing the efficacy of fungicides against different strains of potato late blight. Leaf discs from potato plants were sprayed with different fungicides and inoculated with four Phytophthora infestans isolates. Disease severity was assessed by measuring necrotic foliage and sporulation. Results showed that the efficacy of fungicides depended on both the fungicide used and the P. infestans isolate. Metalaxyl was less effective against an isolate resistant to the fungicide. A fluazinam isolate showed higher disease levels than others under that fungicide. In general, all fungicides reduced disease compared to the untreated control.
Assessing the Risk of Resistance in Pseudoperonospora cubensis to the Fungici...dinomasch
The study assessed the risk of resistance developing in the oomycete fungus Pseudoperonospora cubensis, which causes cucumber downy mildew, to the fungicide flumorph. Researchers compared flumorph to dimethomorph and azoxystrobin in terms of the ease of isolating resistant mutants, the level of resistance induced, and the fitness of resistant mutants. Attempts to induce resistance to flumorph and dimethomorph by adapting sporangia on treated leaves were unsuccessful. Moderately resistant mutants were isolated using UV mutagenesis for flumorph and dimethomorph, but resistance levels were much lower than for azoxystrobin-resistant mutants.
This study investigated the antimicrobial activity of ethanol and chloroform extracts of Spirulina platensis against clinical isolates of Salmonella enterica serovar Typhi and Paratyphi. The ethanol extract showed variable zones of inhibition against the isolates ranging from 9.5-16 mm for S. Typhi and 9.7-14 mm for S. Paratyphi. However, the chloroform extract did not show any inhibitory effect. While the zones of inhibition were smaller than the antibiotic ciprofloxacin, this study provides promising evidence for developing a natural treatment for infections caused by these Salmonella serovars using S. platensis extracts.
a) Methods used in sampling, extraction of motile stages and cysts,
b) Different groups of plant parasitic nematodes found in soil samples,
c) Calculate the ratio of eggs and cysts in soil samples as well as number of nematodes in a 100ml of soil,
d) Effects of plant parasitic nematodes on a susceptible and resistant variety of fodder radish.
Kasahun's presentation on garlic tissue cultureAmare Kassahun
The document summarizes experiments conducted to optimize a protocol for in vitro propagation of garlic (Allium sativum L.) variety Tseday 92 using basal plate culture. Key findings include:
1) The highest shoot initiation percentage (86.66%) was achieved on medium supplemented with 0.5 or 1.5 mg/L BAP.
2) The maximum number of shoots per explant (9 shoots) was obtained from medium with 1 mg/L BAP and 0.5 mg/L NAA.
3) The highest rooting percentage (100%) and number of roots per explant (10.67) was achieved on medium with 0.5 mg/L NAA.
Role of halonil in reducing contamination in culture mediaAlexander Decker
This document discusses the role of halonil in reducing contamination in culture media. It describes three experiments conducted to determine the effect of various concentrations of halonil (0.5%, 1%, 1.5%) on reducing fungal growth when added to potato dextrose agar plates. The results showed that halonil effectively prevented the growth of contaminants and the test fungus Alternaria alternata when used as an amendment to culture media. Halonil is thus concluded to be effective in controlling laboratory contamination without the need for a laminar flow unit.
Sensitivity of pseudomonas species expressing extended spectrumAlexander Decker
The document summarizes a study that investigated the sensitivity of Pseudomonas species expressing Extended Spectrum Beta Lactamase (ESBL) to different solvent fractions of the plant Milletia aboensis.
Key findings:
- 10 isolates of Pseudomonas species expressing ESBL were obtained from animal samples.
- The ethanol, chloroform and methanol fractions of M. aboensis showed antibacterial activity against the ESBL-producing Pseudomonas isolates. The ethanol extract had the lowest minimum inhibitory concentration.
- The ethyl acetate fraction did not show significant antibacterial effects.
The results suggest M. aboensis contains anti-pseudomonal agents that could treat infections caused by
1) The study evaluated the antimicrobial activity of crude culture filtrate and methanol extract of the mushroom Stereum ostrea against both Gram-positive and Gram-negative bacteria.
2) Crude culture filtrate showed higher inhibitory activity compared to methanol extract, as evidenced by larger inhibition zones. The crude filtrate was most effective against Bacillus subtilis and least effective against Klebsiella pneumoniae.
3) The minimal inhibitory concentration of both crude and methanol extracts was 20 μl for Bacillus subtilis and 30 μl for the other bacteria tested, indicating Stereum ostrea was most potent against Bacillus subtilis.
Assessing the Risk of Resistance in Pseudoperonospora cubensis to the Fungici...dinomasch
The study assessed the risk of resistance developing in the oomycete fungus Pseudoperonospora cubensis, which causes cucumber downy mildew, to the fungicide flumorph. Researchers compared flumorph to dimethomorph and azoxystrobin in terms of the ease of isolating resistant mutants, the level of resistance induced, and the fitness of resistant mutants. Attempts to induce resistance to flumorph and dimethomorph by adapting sporangia on treated leaves were unsuccessful. Moderately resistant mutants were isolated using UV mutagenesis for flumorph and dimethomorph, but resistance levels were much lower than for azoxystrobin-resistant mutants.
This study investigated the antimicrobial activity of ethanol and chloroform extracts of Spirulina platensis against clinical isolates of Salmonella enterica serovar Typhi and Paratyphi. The ethanol extract showed variable zones of inhibition against the isolates ranging from 9.5-16 mm for S. Typhi and 9.7-14 mm for S. Paratyphi. However, the chloroform extract did not show any inhibitory effect. While the zones of inhibition were smaller than the antibiotic ciprofloxacin, this study provides promising evidence for developing a natural treatment for infections caused by these Salmonella serovars using S. platensis extracts.
a) Methods used in sampling, extraction of motile stages and cysts,
b) Different groups of plant parasitic nematodes found in soil samples,
c) Calculate the ratio of eggs and cysts in soil samples as well as number of nematodes in a 100ml of soil,
d) Effects of plant parasitic nematodes on a susceptible and resistant variety of fodder radish.
Kasahun's presentation on garlic tissue cultureAmare Kassahun
The document summarizes experiments conducted to optimize a protocol for in vitro propagation of garlic (Allium sativum L.) variety Tseday 92 using basal plate culture. Key findings include:
1) The highest shoot initiation percentage (86.66%) was achieved on medium supplemented with 0.5 or 1.5 mg/L BAP.
2) The maximum number of shoots per explant (9 shoots) was obtained from medium with 1 mg/L BAP and 0.5 mg/L NAA.
3) The highest rooting percentage (100%) and number of roots per explant (10.67) was achieved on medium with 0.5 mg/L NAA.
Role of halonil in reducing contamination in culture mediaAlexander Decker
This document discusses the role of halonil in reducing contamination in culture media. It describes three experiments conducted to determine the effect of various concentrations of halonil (0.5%, 1%, 1.5%) on reducing fungal growth when added to potato dextrose agar plates. The results showed that halonil effectively prevented the growth of contaminants and the test fungus Alternaria alternata when used as an amendment to culture media. Halonil is thus concluded to be effective in controlling laboratory contamination without the need for a laminar flow unit.
Sensitivity of pseudomonas species expressing extended spectrumAlexander Decker
The document summarizes a study that investigated the sensitivity of Pseudomonas species expressing Extended Spectrum Beta Lactamase (ESBL) to different solvent fractions of the plant Milletia aboensis.
Key findings:
- 10 isolates of Pseudomonas species expressing ESBL were obtained from animal samples.
- The ethanol, chloroform and methanol fractions of M. aboensis showed antibacterial activity against the ESBL-producing Pseudomonas isolates. The ethanol extract had the lowest minimum inhibitory concentration.
- The ethyl acetate fraction did not show significant antibacterial effects.
The results suggest M. aboensis contains anti-pseudomonal agents that could treat infections caused by
1) The study evaluated the antimicrobial activity of crude culture filtrate and methanol extract of the mushroom Stereum ostrea against both Gram-positive and Gram-negative bacteria.
2) Crude culture filtrate showed higher inhibitory activity compared to methanol extract, as evidenced by larger inhibition zones. The crude filtrate was most effective against Bacillus subtilis and least effective against Klebsiella pneumoniae.
3) The minimal inhibitory concentration of both crude and methanol extracts was 20 μl for Bacillus subtilis and 30 μl for the other bacteria tested, indicating Stereum ostrea was most potent against Bacillus subtilis.
Antimicrobial Activity of Leaf Extracts of Asparagus Racemosus Willd–A Medici...IJSTA
The document summarizes a study that evaluated the antimicrobial activity of leaf extracts of Asparagus racemosus Willd, a medicinal plant, against various bacteria and fungi. Crude extracts were obtained from the plant's leaves using solvents like petroleum ether, methanol, chloroform, acetone, ethyl acetate, and water. The effect of these extracts was tested on gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus), gram-negative bacteria (E. coli, Pseudomonas), and the yeast Candida utilis using an agar well diffusion method. The methanol extract showed the highest antimicrobial activity. The study supports the traditional use of the whole plant as a
ABSTRACT- In the present study, sandwich method was used to study the allelopathic interactions of Ipomoea carnea
on two test weed seeds i.e. Amaranthus spinosus and Cassia fistula. Pot experiments were also conducted where
Ipomoea extracts were applied on germinated seedlings in bags and the effect was observed after regular application of
Ipomoea extract as a weedicide. Both methods showed inhibition of the weeds with respect to growth of seedlings.
However, the results were more significant in Sandwich method as compared to Spray Bioassay, indicating the
allelopathic properties of Ipomoea carnea are more significant on un-germinated seeds compared to grown plantlets.
HPTLC analysis revealed the presence of flavonoids, phenols, tannins and terpenoids in Ipomoea carnea. Since all the
four phytochemicals were present in Ipomoea carnea, these could be responsible for allelopathic properties of Ipomoea
carnea on Amaranthus spinosus and Cassia fistula.
Key-words- Ipomoea carnea, Allelopathy, Weedicide, HPTLC
Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Allium...researchagriculture
Cabbage aphid (Brevicoryne brassicae L.) is one of the most problematic pests in smallholder vegetable production, causing significant yield losses in heavy infestations. Current control strategy focuses on use of synthetic pesticides that consequently lead to decimation of natural enemies, development of insect resistance and resurgence and upset biodiversity. Botanical pesticides have been used widely in smallholder farmers but not much documented literature exists on efficacy of these products. A field trial was done to assess the efficacy of crude aqueous extracts of Tephrosia vogelii, Allium sativum and Solanum incanum in controlling Brevicoryne brassicae in Brassica napus production. The trial was laid in a randomized complete block design (RCBD) with five treatments replicated four times. The five treatments used in the experiment were T. vogelii, A. sativum, S. incanum, dimethoate and control. Wingless adult female aphids were inoculated three weeks after transplanting of seedlings. Spraying and data collection were done weekly for four weeks. Data was collected on aphid nymph and adult counts on the third leaf from the aerial plant part of randomly selected plants from each treatment for 24 hours after the application of treatments and total plant fresh weight per each treatment. There were significant differences (p<0.05)><0.05) on the yield of rape. It was concluded that T. vogelii, S. incanum and A. sativum aqueous crude extracts have some pesticidal effects on aphid in rape production.
Article Citation:
Shepherd Mudzingwa, Simbarashe Muzemu and James Chitamba.
Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Allium sativum L. and Solanum incanum L. in controlling aphids (Brevicoryne brassicae L.) in rape (Brassica napus L.)
Journal of Research in Agriculture (2013) 2(1): 157-163.
Full Text:
http://www.jagri.info/documents/AG0040.pdf
Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Alli...researchagriculture
Cabbage aphid (
Brevicoryne brassicae
L.) is one of the most problematic
pests in smallholder vegetable production, causing significant yield losses in heavy
infestations. Current control strategy focuses on use of synthetic pesticides that
consequently lead to decimation of natural enemies, development of insect
resistance and resurgence and upset biodiversity. Botanical pesticides have been used
widely in smallholder farmers but not much documented literature exists on efficacy
of these products. A field trial was done to assess the efficacy of crude aqueous
extracts of
Tephrosia vogelii
,
Allium sativum
and
Solanum incanum
in controlling
Brevicoryne brassicae
in
Brassica napus
production. The trial was laid in a randomized
complete block design (RCBD) with five treatments replicated four times. The five
treatments used in the experiment were
T
.
vogelii
,
A
.
sativum
,
S
.
incanum
,
dimethoate and control. Wingless adult female aphids were inoculated three weeks
after transplanting of seedlings. Spraying and data collection were done weekly for
four weeks. Data was collected on aphid nymph and adult counts on the third leaf
from the aerial plant part of randomly selected plants from each treatment for
24 hours after the application of treatments and total plant fresh weight per each
treatment. There were significant differences (p<0.05)><0.05) on the yield of rape. It was concluded that
T. vogelii
,
S
.
incanum
and
A
.
sativum
aqueous crude extracts have some pesticidal
effects on aphid in rape
production.
In vitro Antimicrobial Activity Screening of Rheum rhabarbarum Rootsinventionjournals
Rheum rhabarbarum, which is commonly known as rhubarb, has been used as a medicinal herb in different countries. Especially its roots are known to be a traditional medicine in different cultures. Mesir paste was prepared about 500 years ago during Ottoman period as a medicinal paste and R. rhabarbarum was one of its ingredients. In this study the in vitro antimicrobial activity of ethanol extract of R. rhabarbarum roots was investigated against 17 bacterial and 1 fungal strain, namely, Bacillus subtilis DSMZ 1971, Candida albicans DSMZ 1386, Enterobacter aerogenes ATCC 13048, Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Klebsiella pneumoniae, Listeria innocula, Listeria monocytogenes ATCC 7644, Pseudomonas aeruginosa DSMZ 50071, Pseudomonas fluorescence P1, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923 and Staphylococcus epidermidis DSMZ 20044 by using the disk diffusion method. It is observed that ethanol extracts of R. rhabarbarum root extracts has antimicrobial activity against all microorganims tested
In vitro studies on Efflux pump Inhibition of Catharanthus roseus and piperin...inventionjournals
This study evaluated the antimycobacterial activity and efflux pump inhibition of Catharanthus roseus and piperine from Piper nigrum against ofloxacin-resistant Mycobacterium tuberculosis. GC-MS analysis identified major components in C. roseus including furfural, 2(5H)-furanone, and hydrastininic acid. Both plant extracts showed inhibitory activity against M. tuberculosis at a minimum inhibitory concentration of 50 μg/ml. Piperine significantly reduced the MIC of ofloxacin, demonstrating efflux pump inhibition, while C. roseus did not affect efflux pumps. The results suggest piperine and C. roseus have antimycobacterial effects and pip
This study investigated the inhibitory effects of aqueous onion and garlic extracts on the growth and keratinase activity of Trichophyton mentagrophytes, a fungus that causes skin infections. Thirty isolates of T. mentagrophytes were tested for their ability to produce keratinase, a key enzyme for fungal invasion. The isolates demonstrated a range of keratinase production levels under optimal culture conditions. Both onion and garlic extracts significantly inhibited the growth of T. mentagrophytes at various concentrations, with up to 100% inhibition at a 10% extract concentration. The extracts also inhibited keratinase activity of the fungus in a dose-dependent manner. The results suggest that onion and garlic extracts have potential for development into anti
Testing the efficacy of fungicides on spore germination of Alternaria solaniUPL
Early blight is caused by Alternaria solani. Although late blight, caused by Phytophthora infestans, is the most important disease in potato production early blight is regularly found in potato fields in the second half of the season. Farmers spray potatoes 2 to 4 times during a season to control early blight.
Antimicrobial activity of callistemon citrinusDeborah Bauer
This document reports on a study that investigated the antimicrobial activity of methanolic extracts of Callistemon citrinus and Callistemon salignus against various bacteria and fungi. The extracts showed inhibitory effects against both gram-positive and gram-negative bacteria, with gram-positive bacteria generally being more susceptible. C. citrinus flower extract inhibited the growth of 64% of bacteria tested and C. salignus flower extract inhibited 43% of bacteria tested. Time-kill assays demonstrated growth inhibition within 1 hour for some bacteria. The extracts displayed low toxicity in an Artemia franciscana toxicity assay. The results validate traditional Aboriginal use of these plants as antiseptic agents and confirm their medicinal potential.
ABSTRACT- The present study was planned to study the antimicrobial activity of different plant extract against selected microorganisms. The plants used in the present study were Ocimum sanctum (Tulsi), Withania somnifera (Ashwgandha), Santalum album (Chandan), Aloe vera (Aloe barbadensis), and shatavari (Asparagus racemosus). The extract from the leaves of these plants (are) used in malaria, bronchitis, gastric disorders, cough, cold etc. To test efficiency of some common plants extract against E. coli, Salmonella typhi, Proteus vulgaris, Staphylococcus aureus. Contrary to the synthetic drugs, antimicrobials of plant origin are not associated with many side effects and have an enormous therapeutic potential to heal many infectious diseases. The present investigation is therefore, undertaken to test the efficiency of some of the common plant extracts against some plants and human pathogens, i.e. E. coli and S. aureus. In this project work, we studied the different parts of medicinal plants of Latur, Osmanabad region used for curing different type of diseases specially skin diseases. Some plants have active components which show antimicrobial activity. These Herbal plants are beneficial to human being in therapeutic practice. Skin diseases are difficult conditions to live with, to save the very least. Though some skin diseases may cause minimal discomfort, the visual effects of the conditions can cause significant self esteem and confidence issues. The majority of skin diseases cause scarring or disfigurement. Skin diseases run the gambit from barely noticeable to fatal.
Key-words- Medicinal plants, Antimicrobial activity, Antifungal activity
Isolation and Screening of Soil Actinomycetes for Antimicrobial Activity OlakunleAjibola
Antimicrobial resistance in pathogens has greatly increased of late and now pose a serious public health problem globally. New antimicrobials are continuously needed to inhibit the growth of these resistant strains.
The aim of this study was to isolate and screen soil actinomycetes and evaluate their secondary metabolites for antimicrobial activities against selected pathogenic bacteria and fungi.
This document discusses a study that investigated the antifungal and anti-mycelium activities of biogenic silver, copper, zinc oxide, and gold nanoparticles. The nanoparticles were tested against four fungal strains (Candida albicans, Cryptococcus neoformans, Aspergillus niger, Fusarium oxysporum). The minimum inhibitory concentrations of silver, copper, and zinc oxide nanoparticles were determined to be ≤8 μg/ml for the non-spore forming fungi and ≤16 μg/ml for the spore forming fungi. Anti-mycelium effects were observed for A. niger and F. oxysporum, with silver nanoparticles showing the highest effect at 72.8%. The
Isolation and Characterization of Bacteria from Tropical SoilsNicole Rivera
This document describes a study that aimed to isolate bacteria from tropical soils in Puerto Rico that have the potential to produce antibiotics. Soil samples were collected and bacteria were isolated through serial dilution and culturing. Four isolated bacteria were purified and characterized through gram staining, testing for antibiotic production against E. coli and M. luteus, PCR amplification with 16S rRNA primers, and DNA sequencing. Two of the bacteria showed potential as antibiotic producers against M. luteus. The isolation and characterization of soil bacteria with antibiotic properties could help address the growing problem of antibiotic resistance.
Management of potato virus Y (PVY) in potato by some biocontrol agents under ...Open Access Research Paper
The study was conducted to test the activity of Pseudomonas fluorescens, Rhodotorula sp and fermented neem extract to protect potato plants against potato virusY disease development under field conditions. Infected potato tubers were soaked in P. fluorescens, Rhodotorula sp suspensions and in fermented neem extracts separately and sown in the field in completely randomized block design. The development of virus symptoms and the accumulation of virus in the plant based on Enzyme Linked Immunosorbent Assay (ELISA) were followed. The results obtained showed that the treatment of potato tubers with the three agents have significantly accelerated plant emergence, 5-6 days early than non treated ones, and improved plant growth, the plant dry weights ranged from 120-177 g/plant compared to 42 g/plant in non treated plants. The enhancement of plant growth was found associated with reduction in disease severity based on symptoms development and restriction of virus concentration as proved by ELISA absorbance of 405 nm, 0.14-0.23 compared with 2.50 in non treated plants. The results indicated that the use of bioagent to induce systemic resistance provide an efficient tool, as insecticide alternative to manage potato virus Y in potato. Check out more by following link https://innspub.net/management-of-potato-virus-y-pvy-in-potato-by-some-biocontrol-agents-under-field-conditions/
Testing the efficacy of fungicides to control different strains of Phytophtho...UPL
Late blight, caused by Phytophthora infestans, is the most important disease in potato production because
of its capacity to destroy foliage very rapidly and its ability to infect tubers. To avoid infection of the foliage,
fungicides are used frequently. In the Netherlands, in some years up to 15 sprays are necessary to prevent
the crop from infection by the pathogen.
a) Methods used in sampling, extraction of motile stages and cysts,
b) Different groups of plant parasitic nematodes found in soil samples,
c) Calculate the ratio of eggs and cysts in soil samples as well as number of nematodes in a 100ml of soil,
d) Effects of plant parasitic nematodes on a susceptible and resistant variety of fodder radish.
Early blight of potato caused by Alternaria alternata (Fries) Keissler is one of the factors that affect
potato production. Using chemical control to reduce disease severity represent another risk for
agriculture. Biological control, using microorganisms, as well as plant extract and compost are safe
measures that give reliable control. Applying either measure of control reduced disease incidence to less
than 2% during two successive seasons. Microbial spray of diseased potato reduced the disease to various
extents. Trichoderma sp. was the most effective among all tested microorganisms, followed by
Penicillium sp. On the other hand, alcoholic extract of three plants was more effective than their water
extract. However, water extract showed highly significant reduction of disease incidence. Polygonum
gave the highest reduction of disease incidence in both cases. Also water extract of compost greatly
suppressed early blight when applied as spray to potato leaves. Duration of extraction may affect the
ability of compost extract to suppress disease. All results were comparable to that of fungicides.
The Effect of Dipel and Spruzit Biopesticides on Metcalfa pruinosa (Say, 1830...IJEAB
Metcalfa pruinosa (Say) is one of the important harmful insect species of the coastal areas of Eastern Black Sea Region. This insect poses a danger by feeding on the juices of hundreds of plants in the region. This study was designed to create a fight strategy against M.pruinosa, which has posed an intense danger in Artvin - Kemalpaşa in recent years, and the effect of Spruzit Neu and Dipel biopesticides on the nymphs and adults of the insect was investigated, and the applicability of these biopesticides was revealed. The study was conducted in the summer season of 2016 when the nymphs and adults of the insect are abundant in the region. In in vitro conditions, the Spruzit Neu (Pyrethrum) and Dipel DF (Bacillu thuringiensis) biopesticides were sprayed at different doses (DiPel® DF BT 100gr / 100lt, Dipel® DF BT 300gr / 100lt, Dipel® DF BT 500gr / 100lt ve Spruzit® Neu) on the nymphs and adults of the insect. The adults and nymphs were checked with 2-day intervals, and the results were assessed according to the One-Way Variance Analysis (ANOVA) and the Duncan Test. It was determined that the most effective applications for the nymphs were Dipel DF 300gr/100lt and 500gr/100lt. It was also determined that the most effective applications for the adult individuals were Spruzit Neu 600ml/100lt and Dipel DF 500gr/100lt doses. The highest death rates in the nymphs were determined in Spruzit Neu 600ml/100lt dose as 72,5%; and in Dipel DF dose as 80%. These rates were determined in Pyrethrum 600 ml/100lt dose as 78%, and in Dipel DF 500 gr/100lt dose as 75%. As a conclusion, it was determined that both biopesticides are influential on the nymphs and adults of M.pruinosa. However, it was also determined that the fight will be more influential in the nymph period of the insect.
— The damping off disease of tobacco seedlings caused by the pathogen Rhizoctonia solani causes a huge damage. The limited numbers of products are used in protection from the disease, but lately new fungicides appeared on the market. The aim of these investigations was to determine the effectiveness of new fungicides in control of this pathogen, compared with commonly used. The tests were carried out in conditions in vitro, with 8 fungicides. Two fungicides were evaluated in 2 or 3 concentrations. All tested fungicides showed extremely high effectiveness in the control of this pathogenic fungus –the percentage of inhibition of radial growth ranges from 80, 45 to 100%. The preparate Orvego (300 g / l ametoctradin + 225 g / l dimetomorph) is exception –it showed 48.05%. The best effectiveness showed contact fungicides Manfil 80WP (800 g/kg mancozeb) and Enervin WG (120 g/kg ametotradin +440 g/kg metiram) as well as systemic Signum 33 WG (267 g/kg boscalid +67g/kg pyraclostrobin) and Quadris 25 SC (250 g/l azoxystrobin)with100%inhibition of pathogenʼs development. Fungicides with such high effectiveness in control of R.solani will ensure their use in protection of tobacco seedling from the damping off disease.
A pot experiment was conducted to evaluate the nematicidal efficacy of a biocontrol agent, Pseudomonas flourescens for the management of root-knot nematode, Meloidogyneincognita on chickpea (Cicer arietinum L.) cv.‘Avarodhi’ under glasshouse conditions. All the treatments were found to significantly improve the growth and physiological parameters of chickpea and reduction in pathological parameters as compare to untreated inoculated control. The highest improvement was observed in those plants treated with P. flourescens alone. Concomitant and sequential inoculation of P. flourescens with M.incognita also showed significant improvement in growth parameters of chickpea. Least enhancement in growth parameters was observed in those plants inoculated with nematode alone. It may be due to the nematcidal behaviour of P. flourescens against root-knot nematode, M. incognita. Hence, it may be concluded that P. flourescens as biocontrol agent is better substitute against chemical nematicides for the sustainable management of M. incognita and reduce environmental hazards.
ABSTRACT- Mosquito-borne diseases have intruded the globe since immemorial time. The present scenario for
commanding the mosquitoes is aimed at application of target and stage-specific, cost-effective and biodegradable
phytoproducts. Plant extracts are safer for non-target organisms including man. Plant based formulations would be more
feasible environmental products with proven potential as insecticide. Therefore, in the present study of larvicidal
activity of biologically active compound Apigenin extracted from leaf of Jatropha gossypifolia against the filarial
vector, Culex quinquefasciatus was studied. Standard WHO protocols with minor modifications was adopted for the
larvicidal bioassay. The active compound Apigenin extracted through ethyl alcohol solvent from the leaf of Jatropha
gossypifolia plant of family Euphorbiaceae was administered for 24h or 96h to the larvae of Culex quinquefasciatus.
Exposure of larvae over 24h to sub-lethal doses (40% and 80% of LC50) of apigenin, significantly (P<0.05) altered the
level of total protein, total free amino acid, glycogen and activity of enzymes acetyl cholinesterase, acid and alkaline
phosphatase activity in whole body tissue of Culex quinquefasciatus larvae. The alterations in all these biochemical
parameters were significantly (P<0.05) time and dose dependent.
Key-words- Jatropha gossypifolia, Euphorbiaceae, Culex quinquefasciatus, biochemical effects, Wuchereria bancrofti
Antimicrobial Activity of Leaf Extracts of Asparagus Racemosus Willd–A Medici...IJSTA
The document summarizes a study that evaluated the antimicrobial activity of leaf extracts of Asparagus racemosus Willd, a medicinal plant, against various bacteria and fungi. Crude extracts were obtained from the plant's leaves using solvents like petroleum ether, methanol, chloroform, acetone, ethyl acetate, and water. The effect of these extracts was tested on gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus), gram-negative bacteria (E. coli, Pseudomonas), and the yeast Candida utilis using an agar well diffusion method. The methanol extract showed the highest antimicrobial activity. The study supports the traditional use of the whole plant as a
ABSTRACT- In the present study, sandwich method was used to study the allelopathic interactions of Ipomoea carnea
on two test weed seeds i.e. Amaranthus spinosus and Cassia fistula. Pot experiments were also conducted where
Ipomoea extracts were applied on germinated seedlings in bags and the effect was observed after regular application of
Ipomoea extract as a weedicide. Both methods showed inhibition of the weeds with respect to growth of seedlings.
However, the results were more significant in Sandwich method as compared to Spray Bioassay, indicating the
allelopathic properties of Ipomoea carnea are more significant on un-germinated seeds compared to grown plantlets.
HPTLC analysis revealed the presence of flavonoids, phenols, tannins and terpenoids in Ipomoea carnea. Since all the
four phytochemicals were present in Ipomoea carnea, these could be responsible for allelopathic properties of Ipomoea
carnea on Amaranthus spinosus and Cassia fistula.
Key-words- Ipomoea carnea, Allelopathy, Weedicide, HPTLC
Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Allium...researchagriculture
Cabbage aphid (Brevicoryne brassicae L.) is one of the most problematic pests in smallholder vegetable production, causing significant yield losses in heavy infestations. Current control strategy focuses on use of synthetic pesticides that consequently lead to decimation of natural enemies, development of insect resistance and resurgence and upset biodiversity. Botanical pesticides have been used widely in smallholder farmers but not much documented literature exists on efficacy of these products. A field trial was done to assess the efficacy of crude aqueous extracts of Tephrosia vogelii, Allium sativum and Solanum incanum in controlling Brevicoryne brassicae in Brassica napus production. The trial was laid in a randomized complete block design (RCBD) with five treatments replicated four times. The five treatments used in the experiment were T. vogelii, A. sativum, S. incanum, dimethoate and control. Wingless adult female aphids were inoculated three weeks after transplanting of seedlings. Spraying and data collection were done weekly for four weeks. Data was collected on aphid nymph and adult counts on the third leaf from the aerial plant part of randomly selected plants from each treatment for 24 hours after the application of treatments and total plant fresh weight per each treatment. There were significant differences (p<0.05)><0.05) on the yield of rape. It was concluded that T. vogelii, S. incanum and A. sativum aqueous crude extracts have some pesticidal effects on aphid in rape production.
Article Citation:
Shepherd Mudzingwa, Simbarashe Muzemu and James Chitamba.
Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Allium sativum L. and Solanum incanum L. in controlling aphids (Brevicoryne brassicae L.) in rape (Brassica napus L.)
Journal of Research in Agriculture (2013) 2(1): 157-163.
Full Text:
http://www.jagri.info/documents/AG0040.pdf
Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Alli...researchagriculture
Cabbage aphid (
Brevicoryne brassicae
L.) is one of the most problematic
pests in smallholder vegetable production, causing significant yield losses in heavy
infestations. Current control strategy focuses on use of synthetic pesticides that
consequently lead to decimation of natural enemies, development of insect
resistance and resurgence and upset biodiversity. Botanical pesticides have been used
widely in smallholder farmers but not much documented literature exists on efficacy
of these products. A field trial was done to assess the efficacy of crude aqueous
extracts of
Tephrosia vogelii
,
Allium sativum
and
Solanum incanum
in controlling
Brevicoryne brassicae
in
Brassica napus
production. The trial was laid in a randomized
complete block design (RCBD) with five treatments replicated four times. The five
treatments used in the experiment were
T
.
vogelii
,
A
.
sativum
,
S
.
incanum
,
dimethoate and control. Wingless adult female aphids were inoculated three weeks
after transplanting of seedlings. Spraying and data collection were done weekly for
four weeks. Data was collected on aphid nymph and adult counts on the third leaf
from the aerial plant part of randomly selected plants from each treatment for
24 hours after the application of treatments and total plant fresh weight per each
treatment. There were significant differences (p<0.05)><0.05) on the yield of rape. It was concluded that
T. vogelii
,
S
.
incanum
and
A
.
sativum
aqueous crude extracts have some pesticidal
effects on aphid in rape
production.
In vitro Antimicrobial Activity Screening of Rheum rhabarbarum Rootsinventionjournals
Rheum rhabarbarum, which is commonly known as rhubarb, has been used as a medicinal herb in different countries. Especially its roots are known to be a traditional medicine in different cultures. Mesir paste was prepared about 500 years ago during Ottoman period as a medicinal paste and R. rhabarbarum was one of its ingredients. In this study the in vitro antimicrobial activity of ethanol extract of R. rhabarbarum roots was investigated against 17 bacterial and 1 fungal strain, namely, Bacillus subtilis DSMZ 1971, Candida albicans DSMZ 1386, Enterobacter aerogenes ATCC 13048, Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Klebsiella pneumoniae, Listeria innocula, Listeria monocytogenes ATCC 7644, Pseudomonas aeruginosa DSMZ 50071, Pseudomonas fluorescence P1, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923 and Staphylococcus epidermidis DSMZ 20044 by using the disk diffusion method. It is observed that ethanol extracts of R. rhabarbarum root extracts has antimicrobial activity against all microorganims tested
In vitro studies on Efflux pump Inhibition of Catharanthus roseus and piperin...inventionjournals
This study evaluated the antimycobacterial activity and efflux pump inhibition of Catharanthus roseus and piperine from Piper nigrum against ofloxacin-resistant Mycobacterium tuberculosis. GC-MS analysis identified major components in C. roseus including furfural, 2(5H)-furanone, and hydrastininic acid. Both plant extracts showed inhibitory activity against M. tuberculosis at a minimum inhibitory concentration of 50 μg/ml. Piperine significantly reduced the MIC of ofloxacin, demonstrating efflux pump inhibition, while C. roseus did not affect efflux pumps. The results suggest piperine and C. roseus have antimycobacterial effects and pip
This study investigated the inhibitory effects of aqueous onion and garlic extracts on the growth and keratinase activity of Trichophyton mentagrophytes, a fungus that causes skin infections. Thirty isolates of T. mentagrophytes were tested for their ability to produce keratinase, a key enzyme for fungal invasion. The isolates demonstrated a range of keratinase production levels under optimal culture conditions. Both onion and garlic extracts significantly inhibited the growth of T. mentagrophytes at various concentrations, with up to 100% inhibition at a 10% extract concentration. The extracts also inhibited keratinase activity of the fungus in a dose-dependent manner. The results suggest that onion and garlic extracts have potential for development into anti
Testing the efficacy of fungicides on spore germination of Alternaria solaniUPL
Early blight is caused by Alternaria solani. Although late blight, caused by Phytophthora infestans, is the most important disease in potato production early blight is regularly found in potato fields in the second half of the season. Farmers spray potatoes 2 to 4 times during a season to control early blight.
Antimicrobial activity of callistemon citrinusDeborah Bauer
This document reports on a study that investigated the antimicrobial activity of methanolic extracts of Callistemon citrinus and Callistemon salignus against various bacteria and fungi. The extracts showed inhibitory effects against both gram-positive and gram-negative bacteria, with gram-positive bacteria generally being more susceptible. C. citrinus flower extract inhibited the growth of 64% of bacteria tested and C. salignus flower extract inhibited 43% of bacteria tested. Time-kill assays demonstrated growth inhibition within 1 hour for some bacteria. The extracts displayed low toxicity in an Artemia franciscana toxicity assay. The results validate traditional Aboriginal use of these plants as antiseptic agents and confirm their medicinal potential.
ABSTRACT- The present study was planned to study the antimicrobial activity of different plant extract against selected microorganisms. The plants used in the present study were Ocimum sanctum (Tulsi), Withania somnifera (Ashwgandha), Santalum album (Chandan), Aloe vera (Aloe barbadensis), and shatavari (Asparagus racemosus). The extract from the leaves of these plants (are) used in malaria, bronchitis, gastric disorders, cough, cold etc. To test efficiency of some common plants extract against E. coli, Salmonella typhi, Proteus vulgaris, Staphylococcus aureus. Contrary to the synthetic drugs, antimicrobials of plant origin are not associated with many side effects and have an enormous therapeutic potential to heal many infectious diseases. The present investigation is therefore, undertaken to test the efficiency of some of the common plant extracts against some plants and human pathogens, i.e. E. coli and S. aureus. In this project work, we studied the different parts of medicinal plants of Latur, Osmanabad region used for curing different type of diseases specially skin diseases. Some plants have active components which show antimicrobial activity. These Herbal plants are beneficial to human being in therapeutic practice. Skin diseases are difficult conditions to live with, to save the very least. Though some skin diseases may cause minimal discomfort, the visual effects of the conditions can cause significant self esteem and confidence issues. The majority of skin diseases cause scarring or disfigurement. Skin diseases run the gambit from barely noticeable to fatal.
Key-words- Medicinal plants, Antimicrobial activity, Antifungal activity
Isolation and Screening of Soil Actinomycetes for Antimicrobial Activity OlakunleAjibola
Antimicrobial resistance in pathogens has greatly increased of late and now pose a serious public health problem globally. New antimicrobials are continuously needed to inhibit the growth of these resistant strains.
The aim of this study was to isolate and screen soil actinomycetes and evaluate their secondary metabolites for antimicrobial activities against selected pathogenic bacteria and fungi.
This document discusses a study that investigated the antifungal and anti-mycelium activities of biogenic silver, copper, zinc oxide, and gold nanoparticles. The nanoparticles were tested against four fungal strains (Candida albicans, Cryptococcus neoformans, Aspergillus niger, Fusarium oxysporum). The minimum inhibitory concentrations of silver, copper, and zinc oxide nanoparticles were determined to be ≤8 μg/ml for the non-spore forming fungi and ≤16 μg/ml for the spore forming fungi. Anti-mycelium effects were observed for A. niger and F. oxysporum, with silver nanoparticles showing the highest effect at 72.8%. The
Isolation and Characterization of Bacteria from Tropical SoilsNicole Rivera
This document describes a study that aimed to isolate bacteria from tropical soils in Puerto Rico that have the potential to produce antibiotics. Soil samples were collected and bacteria were isolated through serial dilution and culturing. Four isolated bacteria were purified and characterized through gram staining, testing for antibiotic production against E. coli and M. luteus, PCR amplification with 16S rRNA primers, and DNA sequencing. Two of the bacteria showed potential as antibiotic producers against M. luteus. The isolation and characterization of soil bacteria with antibiotic properties could help address the growing problem of antibiotic resistance.
Management of potato virus Y (PVY) in potato by some biocontrol agents under ...Open Access Research Paper
The study was conducted to test the activity of Pseudomonas fluorescens, Rhodotorula sp and fermented neem extract to protect potato plants against potato virusY disease development under field conditions. Infected potato tubers were soaked in P. fluorescens, Rhodotorula sp suspensions and in fermented neem extracts separately and sown in the field in completely randomized block design. The development of virus symptoms and the accumulation of virus in the plant based on Enzyme Linked Immunosorbent Assay (ELISA) were followed. The results obtained showed that the treatment of potato tubers with the three agents have significantly accelerated plant emergence, 5-6 days early than non treated ones, and improved plant growth, the plant dry weights ranged from 120-177 g/plant compared to 42 g/plant in non treated plants. The enhancement of plant growth was found associated with reduction in disease severity based on symptoms development and restriction of virus concentration as proved by ELISA absorbance of 405 nm, 0.14-0.23 compared with 2.50 in non treated plants. The results indicated that the use of bioagent to induce systemic resistance provide an efficient tool, as insecticide alternative to manage potato virus Y in potato. Check out more by following link https://innspub.net/management-of-potato-virus-y-pvy-in-potato-by-some-biocontrol-agents-under-field-conditions/
Testing the efficacy of fungicides to control different strains of Phytophtho...UPL
Late blight, caused by Phytophthora infestans, is the most important disease in potato production because
of its capacity to destroy foliage very rapidly and its ability to infect tubers. To avoid infection of the foliage,
fungicides are used frequently. In the Netherlands, in some years up to 15 sprays are necessary to prevent
the crop from infection by the pathogen.
a) Methods used in sampling, extraction of motile stages and cysts,
b) Different groups of plant parasitic nematodes found in soil samples,
c) Calculate the ratio of eggs and cysts in soil samples as well as number of nematodes in a 100ml of soil,
d) Effects of plant parasitic nematodes on a susceptible and resistant variety of fodder radish.
Early blight of potato caused by Alternaria alternata (Fries) Keissler is one of the factors that affect
potato production. Using chemical control to reduce disease severity represent another risk for
agriculture. Biological control, using microorganisms, as well as plant extract and compost are safe
measures that give reliable control. Applying either measure of control reduced disease incidence to less
than 2% during two successive seasons. Microbial spray of diseased potato reduced the disease to various
extents. Trichoderma sp. was the most effective among all tested microorganisms, followed by
Penicillium sp. On the other hand, alcoholic extract of three plants was more effective than their water
extract. However, water extract showed highly significant reduction of disease incidence. Polygonum
gave the highest reduction of disease incidence in both cases. Also water extract of compost greatly
suppressed early blight when applied as spray to potato leaves. Duration of extraction may affect the
ability of compost extract to suppress disease. All results were comparable to that of fungicides.
The Effect of Dipel and Spruzit Biopesticides on Metcalfa pruinosa (Say, 1830...IJEAB
Metcalfa pruinosa (Say) is one of the important harmful insect species of the coastal areas of Eastern Black Sea Region. This insect poses a danger by feeding on the juices of hundreds of plants in the region. This study was designed to create a fight strategy against M.pruinosa, which has posed an intense danger in Artvin - Kemalpaşa in recent years, and the effect of Spruzit Neu and Dipel biopesticides on the nymphs and adults of the insect was investigated, and the applicability of these biopesticides was revealed. The study was conducted in the summer season of 2016 when the nymphs and adults of the insect are abundant in the region. In in vitro conditions, the Spruzit Neu (Pyrethrum) and Dipel DF (Bacillu thuringiensis) biopesticides were sprayed at different doses (DiPel® DF BT 100gr / 100lt, Dipel® DF BT 300gr / 100lt, Dipel® DF BT 500gr / 100lt ve Spruzit® Neu) on the nymphs and adults of the insect. The adults and nymphs were checked with 2-day intervals, and the results were assessed according to the One-Way Variance Analysis (ANOVA) and the Duncan Test. It was determined that the most effective applications for the nymphs were Dipel DF 300gr/100lt and 500gr/100lt. It was also determined that the most effective applications for the adult individuals were Spruzit Neu 600ml/100lt and Dipel DF 500gr/100lt doses. The highest death rates in the nymphs were determined in Spruzit Neu 600ml/100lt dose as 72,5%; and in Dipel DF dose as 80%. These rates were determined in Pyrethrum 600 ml/100lt dose as 78%, and in Dipel DF 500 gr/100lt dose as 75%. As a conclusion, it was determined that both biopesticides are influential on the nymphs and adults of M.pruinosa. However, it was also determined that the fight will be more influential in the nymph period of the insect.
— The damping off disease of tobacco seedlings caused by the pathogen Rhizoctonia solani causes a huge damage. The limited numbers of products are used in protection from the disease, but lately new fungicides appeared on the market. The aim of these investigations was to determine the effectiveness of new fungicides in control of this pathogen, compared with commonly used. The tests were carried out in conditions in vitro, with 8 fungicides. Two fungicides were evaluated in 2 or 3 concentrations. All tested fungicides showed extremely high effectiveness in the control of this pathogenic fungus –the percentage of inhibition of radial growth ranges from 80, 45 to 100%. The preparate Orvego (300 g / l ametoctradin + 225 g / l dimetomorph) is exception –it showed 48.05%. The best effectiveness showed contact fungicides Manfil 80WP (800 g/kg mancozeb) and Enervin WG (120 g/kg ametotradin +440 g/kg metiram) as well as systemic Signum 33 WG (267 g/kg boscalid +67g/kg pyraclostrobin) and Quadris 25 SC (250 g/l azoxystrobin)with100%inhibition of pathogenʼs development. Fungicides with such high effectiveness in control of R.solani will ensure their use in protection of tobacco seedling from the damping off disease.
A pot experiment was conducted to evaluate the nematicidal efficacy of a biocontrol agent, Pseudomonas flourescens for the management of root-knot nematode, Meloidogyneincognita on chickpea (Cicer arietinum L.) cv.‘Avarodhi’ under glasshouse conditions. All the treatments were found to significantly improve the growth and physiological parameters of chickpea and reduction in pathological parameters as compare to untreated inoculated control. The highest improvement was observed in those plants treated with P. flourescens alone. Concomitant and sequential inoculation of P. flourescens with M.incognita also showed significant improvement in growth parameters of chickpea. Least enhancement in growth parameters was observed in those plants inoculated with nematode alone. It may be due to the nematcidal behaviour of P. flourescens against root-knot nematode, M. incognita. Hence, it may be concluded that P. flourescens as biocontrol agent is better substitute against chemical nematicides for the sustainable management of M. incognita and reduce environmental hazards.
ABSTRACT- Mosquito-borne diseases have intruded the globe since immemorial time. The present scenario for
commanding the mosquitoes is aimed at application of target and stage-specific, cost-effective and biodegradable
phytoproducts. Plant extracts are safer for non-target organisms including man. Plant based formulations would be more
feasible environmental products with proven potential as insecticide. Therefore, in the present study of larvicidal
activity of biologically active compound Apigenin extracted from leaf of Jatropha gossypifolia against the filarial
vector, Culex quinquefasciatus was studied. Standard WHO protocols with minor modifications was adopted for the
larvicidal bioassay. The active compound Apigenin extracted through ethyl alcohol solvent from the leaf of Jatropha
gossypifolia plant of family Euphorbiaceae was administered for 24h or 96h to the larvae of Culex quinquefasciatus.
Exposure of larvae over 24h to sub-lethal doses (40% and 80% of LC50) of apigenin, significantly (P<0.05) altered the
level of total protein, total free amino acid, glycogen and activity of enzymes acetyl cholinesterase, acid and alkaline
phosphatase activity in whole body tissue of Culex quinquefasciatus larvae. The alterations in all these biochemical
parameters were significantly (P<0.05) time and dose dependent.
Key-words- Jatropha gossypifolia, Euphorbiaceae, Culex quinquefasciatus, biochemical effects, Wuchereria bancrofti
Anti-Oxidant and Antimicrobial Studies of Tinospora cordifolia (Guduchi/Giloy...SUS GROUP OF INSTITUTIONS
Plants produce a diverse range of bioactive molecules, making them a rich source of
different types of medicines and healing properties. The present study was aimed to
evaluate the anti-oxidant and antimicrobial properties of stem and root of T. cordifolia.
Total phenolic contents of different solvent extracts were determined and found that ethanol
extract had the highest phenolic content of 0.3213 mg g-1. Antioxidant assays were also
carried out by using different in vitro models such as total reducing power, hydrogen
peroxide scavenging activity assay and hydroxyl redical scavenging activity. The Ethanol
extract showed the highest total antioxidant activity. The H2O2 scavenging and hydroxyl
free radical scavenging activity was maximum 87.2 % and 91.0% found in case of ethanolic
steam extract respectively. The antimicrobial activity of ethanolic and methanolic extract of
root and stem of T. cordifolia were also evaluated against some pathogenic microorganisms
viz. E. coli, B. subtilis, A. niger and Candida sp. it was found that the various concentration
of extract viz. 50, 100, 150 and 200 mg ml-1 were tested. It was observed that the
increasing in concentration there was also increasing in antimicrobial activity reveled by
increase in size of zone of inhibition. The methanolic stem extract exhibits highest
antimicrobial activity against all four pathogens. The study shown that the extract of T.
cordifolia has a wide range of anti-oxidant as well as antimicrobial activity against bacterial
as well as fungal pathogens.
Antimicrobial screening of Alchemilla vulgaris herbs and flowersinventionjournals
Medical herbs have many bioactive component and they are used in microbial treatment since ancient time. Alchemilla vulgaris is one of them and it is important for folkloric medicine in Turkey. A. vulgaris related antimicrobial research isn’t common, therefore herbs and flowers of this medical plant investigation were applied against 17 bacteria and 1 fungi by using disk diffusion method. These microbial strains include Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Staphylococcus and Candida geniuses. The results were presented that A. vulgaris ethanol extract has antimicrobial activity against all tested microbial strains.
— Post-harvest diseases are a major problem for banana yield. Despite treatments with chemical fungicides, a persistence of diseases is noticed. This study aims at proposing a biological control method against banana post-harvest diseases by using banana rachis leachate. The effect of leachate has been tested in vitro on mycelial growth, conidial germination and in vivo on pathogenic fungi virulence. All leachate concentrations (5, 15 and 20%) tested showed antifungal activity on the tested fungi. However, the 20% concentration was more effective with complete inhibition of mycelial growth and conidial germination of all fungi. No symptoms of crown rot and anthracnose were observed after treatment of bananas with leachate. However, with azoxystrobin, the prevalence of crown rot and anthracnose was 60% and 30%, respectively. Banana rachis leachate recorded highly significant reduction of banana finger rot prevalence compared to azoxystrobin. Banana rachis leachate have strong antifungal properties that may be useful to control banana post-harvest disease as a safe alternative option to chemical fungicides Keywords— banana; post-harvest diseases; banana rachis leachate, antifungal activity.
Effect of Compost Extract Fortified with Tempe on Chili Mosaic Virus DiseaseIJEABJ
Mosaic disease caused by multiple infections of viruses in one of the most devastating virus diseases of chili pepper (Capsicum annuum) in Indonesia. Improving plant resistance by treatment with exogenous bioactive compounds is promisingly developed for plant protection in organic chili production. We demonstrated the suppressive effects of a fermented water extract of compost fortified with over-fermented tempe (TCE) on mosaic disease and its aphid vector. TCE was applied weekly by foliar spraying at 0.2 and 2.0 % on potted Capsicum growing in field. The result showed that treated plant sprayed with TNF preparation exhibited a significantly slower disease progression as represented by a lower area under disease progress curve compared to control plant. Disease suppression was obtained at concentration as low as 0.2%. TCE-treated plants were significantly less colonized by Aphis gossypii than control plant.
Antimicrobial efficacy of tulsi leaf extract on periodontalBT91DHRUVPARMAR
This document discusses periodontitis, a severe gum infection caused by certain pathogens like Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. It presents a study that tested the antimicrobial properties of Tulsi extract against these pathogens. The study found that higher concentrations of Tulsi extract (5-10%) inhibited the growth of A. actinomycetemcomitans similarly to the antibiotic doxycycline. However, Tulsi extract showed weaker inhibition of P. gingivalis and Prevotella intermedia compared to doxycycline. The study concludes that Tulsi extract shows promise for treating some oral infections but requires more research to evaluate
This document summarizes a study that tested the effectiveness of fumigating storage rooms for citrus fruits with smoke from two plants (Artemisia herba alba and Peganum harmala) and a chemical product (Deccofenato pot) at inhibiting the viability of spores from the fungus Penicillium spp. The results showed that smoke from A. herba alba at 25g/m3 and P. harmala at 35g/m3 completely inhibited the spores, as did Deccofenato pot at 1g/m3. The plants and chemical product were concluded to be effective alternatives to existing fungicides for disinfecting citrus storage rooms.
The document summarizes a study that tested a novel antifungal drug (Drug A) in a murine model of invasive pulmonary aspergillosis. Mice were infected with Aspergillus fumigatus and then received various doses of Drug A or a positive control, Posaconazole. The mice were divided into groups for assessing fungal burden or survival. Higher doses of Drug A and Posaconazole reduced fungal counts in the lungs, showing the drug's antifungal activity. However, Drug A unexpectedly increased mouse mortality compared to controls, suggesting it may be toxic. The results point to an experimental error requiring the study to be repeated.
Seed treatment with Pseudomonas fluorescens was found to be the most effective in minimizing disease incidence and maximizing yield in chickpeas. It recorded the lowest incidence of wilt disease (11.26%) and root rot (1.43%), as well as a high yield of 7.02 q/ha. Seed treatment with the fungicide Tebuconazole also performed well with low disease incidence and a yield of 6.62 q/ha. Maximum germination (95%) and seedling vigor were observed with Benomyl and Tebuconazole treatments. Overall, treatments with bioagents like P. fluorescens or fungicides effectively controlled seed-borne diseases, improved seed quality parameters, and
Effectiveness of organic substances in the control of powdery mildew (Sphaero...Innspub Net
Powdery mildew (Sphaerotheca fuligenia) is an economically important disease of cucurbits requiring sustainable means of management. A field experiment was set up to evaluate the effectiveness of cattle milk, sodium bicarbonate, dilute acetic acid and garlic in controlling powdery mildew in butternut (Cucurbita moschata pepo). The trial was laid out as a randomized complete block design with 3 replicates and 6 treatments. The treatments were applied at the following concentrations; 1. Cattle milk at 100ml/liter of water, 2. Dilute acetic acid at 6ml/litre of water, 3. Bicarbonate of soda at 10g/litre of water, 4. Garlic at 20g/litre of water, 5. Tubuconazole in the form of folicur at 0.5ml per liter of water and 6. Control which was not sprayed. A significant difference (P<0.01) was recorded at 60days after planting with respect to disease incidence and milk had the lowest incidence of 12.9% and the highest incidence of 37.3% was recorded in the control treatment. Milk and sodium bicarbonate managed to suppress both disease incidence and severity. This study confirms the fungicidal properties of milk and sodium bicarbonate, garlic, and dilute acetic, suggesting their use as fungicides for disease control for sustainable powdery mildew management. Get the full articles at: https://goo.gl/A2g1tv
Comparative studies of Zingiber officinale leaves and rhizomes on the antibac...pharmaindexing
This study compared the antibacterial effects of ginger (Zingiber officinale) leaves and rhizomes. Extracts were prepared from the leaves and rhizomes using soxhlet extraction. The extracts were tested against four bacterial strains at three concentrations using disc diffusion assays. The rhizome extract showed stronger antibacterial activity than the leaf extract, with the highest zone of inhibition observed for the rhizome extract against Bacillus spp. at a concentration of 100mg/ml. Overall, the rhizome extract demonstrated more potential as an antibacterial agent compared to the leaf extract.
Effect of phytobiocides in controlling soft rot of tomatoAlexander Decker
Turmeric was the most effective phytobiocide for controlling soft rot of tomato caused by Erwinia carotovora carotovora in both laboratory and screen house experiments. In laboratory tests, turmeric produced the largest zone of inhibition against the bacteria, followed by neem and garlic. In screen house studies, tomato plants treated with turmeric grew taller and produced more fresh shoot weight than those treated with other phytobiocides or the control, indicating turmeric was best at controlling the disease and promoting plant growth. Specifically, turmeric treated plants reached 61.67 cm in height and produced 35.68 g of fresh shoot weight, more than any other treatment.
Effect of phytobiocides in controlling soft rot of tomatoAlexander Decker
1) The study tested various phytobiocides (plant-derived pesticides), including turmeric, neem, garlic, mint, oleander, and chili, for their ability to inhibit the growth of Erwinia carotovora carotovora bacteria, which causes soft rot disease in tomatoes.
2) Turmeric produced the largest zone of bacterial growth inhibition in laboratory tests, followed by neem and garlic. Screen house tests found turmeric-treated tomato plants had the highest disease resistance and growth.
3) The results suggest that phytobiocides like turmeric could serve as effective and natural agents for controlling soft rot disease in tomatoes. Turmeric was the most potent inhibitor of the bacteria.
Similar to Testing the efficacy of different fungicides to control different strains of potato late bight (20)
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Now, it’s time to choose new Interline herbicide from UPI. Interline provides a different mode-of-action that controls tough resistant weeds like pigweed, waterhemp, marestail and ragweed.
Este boletim técnico descreve as características do herbicida glufosinato e seu modo de ação, além de fornecer recomendações sobre o manejo de resistência de plantas daninhas. O glufosinato inibe a enzima glutamina sintetase, essencial para o metabolismo do nitrogênio nas plantas, e tem um amplo espectro de controle de daninhas. Seu uso vem aumentando devido à seleção de biótipos resistentes a outros herbicidas e ao desenvolvimento de culturas geneticamente mod
UPL has transformed from a local Indian company into a global agrochemical player over the past 50 years. It has a presence in over 130 countries and revenues of $2.7 billion in FY2018. UPL pursues growth through both organic initiatives such as new product development and strategic acquisitions. It is well positioned in the industry due to its R&D capabilities, global manufacturing and supply chain, extensive registration network, and market access through owned distribution.
At UPL, we place the “Farmer First” in all that we do and continuously strive to innovate products which benet
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Evaluation of mancozeb activity towards various plant fungal pathogen agents ...UPL
Tests were carried out in petri dishes and concern the evaluation of conidial germination of different pathogen populations of Alternaria spp., V. inaequalis and S. vesicarium (for these last two fungi were analyzed two type of strains: sensitive e resistant to strobilurins according to our previous results (Collina et al, 2007 and Fiaccadori et al, 2011). Mancozeb was used as formulated product (solo) dissolved in sterile distillate water.
Importance of multisite fungicides in managing pathogen resistance UPL
The Fungicide Resistance Action Committee (FRAC) is a Specialist Technical Group of CropLife International. The purpose of FRAC is to provide fungicide resistance management guidelines to prolong the effectiveness of "at risk" fungicides and to limit crop losses should resistance occur
Efficacy of fungicides to control Early Blight genotypesUPL
Early Blight is caused by Alternaria solani. Although late blight, caused by Phytophthora infestans, is the most important disease in potato production Early Blight is regularly found in potato fields in the second half of the season. Farmers spray potatoes 2 to 4 times during a season to control Early Blight. Specific fungicides most commonly used in the Netherlands are Signum, Amistar and recently also Narita and Carial Star.
TECHNICAL BULLETIN - FASCINATE THE IDEAL HERBICIDE RESISTANCE PARTNERUPL
This Technical Bulletin provides
information to facilitate the understanding of the growing herbicide resistance of weeds, as well as the consequences of this problem.
Catching Up With Cercospora - Farmers Guide ArticleUPL
Already causing widespread problems on the Continent with resistance to standard sugar beet fungicides, yield robbing disease cercospora has gained momentum in UK beet crops over the past two seasons. Dominic Kilburn reports on this increasing threat as well as the potential availability of a product to help with its control.
UPL Limited and our subsidiaries (together constituting the UPL Group), take initiative to contribute to harmonious and sustainable development of the society through all our business
activities that we carry out in various countries across the globe.
Evolving Lifecycles with High Resolution Site Characterization (HRSC) and 3-D...Joshua Orris
The incorporation of a 3DCSM and completion of HRSC provided a tool for enhanced, data-driven, decisions to support a change in remediation closure strategies. Currently, an approved pilot study has been obtained to shut-down the remediation systems (ISCO, P&T) and conduct a hydraulic study under non-pumping conditions. A separate micro-biological bench scale treatability study was competed that yielded positive results for an emerging innovative technology. As a result, a field pilot study has commenced with results expected in nine-twelve months. With the results of the hydraulic study, field pilot studies and an updated risk assessment leading site monitoring optimization cost lifecycle savings upwards of $15MM towards an alternatively evolved best available technology remediation closure strategy.
Optimizing Post Remediation Groundwater Performance with Enhanced Microbiolog...Joshua Orris
Results of geophysics and pneumatic injection pilot tests during 2003 – 2007 yielded significant positive results for injection delivery design and contaminant mass treatment, resulting in permanent shut-down of an existing groundwater Pump & Treat system.
Accessible source areas were subsequently removed (2011) by soil excavation and treated with the placement of Emulsified Vegetable Oil EVO and zero-valent iron ZVI to accelerate treatment of impacted groundwater in overburden and weathered fractured bedrock. Post pilot test and post remediation groundwater monitoring has included analyses of CVOCs, organic fatty acids, dissolved gases and QuantArray® -Chlor to quantify key microorganisms (e.g., Dehalococcoides, Dehalobacter, etc.) and functional genes (e.g., vinyl chloride reductase, methane monooxygenase, etc.) to assess potential for reductive dechlorination and aerobic cometabolism of CVOCs.
In 2022, the first commercial application of MetaArray™ was performed at the site. MetaArray™ utilizes statistical analysis, such as principal component analysis and multivariate analysis to provide evidence that reductive dechlorination is active or even that it is slowing. This creates actionable data allowing users to save money by making important site management decisions earlier.
The results of the MetaArray™ analysis’ support vector machine (SVM) identified groundwater monitoring wells with a 80% confidence that were characterized as either Limited for Reductive Decholorination or had a High Reductive Reduction Dechlorination potential. The results of MetaArray™ will be used to further optimize the site’s post remediation monitoring program for monitored natural attenuation.
Improving the viability of probiotics by encapsulation methods for developmen...Open Access Research Paper
The popularity of functional foods among scientists and common people has been increasing day by day. Awareness and modernization make the consumer think better regarding food and nutrition. Now a day’s individual knows very well about the relation between food consumption and disease prevalence. Humans have a diversity of microbes in the gut that together form the gut microflora. Probiotics are the health-promoting live microbial cells improve host health through gut and brain connection and fighting against harmful bacteria. Bifidobacterium and Lactobacillus are the two bacterial genera which are considered to be probiotic. These good bacteria are facing challenges of viability. There are so many factors such as sensitivity to heat, pH, acidity, osmotic effect, mechanical shear, chemical components, freezing and storage time as well which affects the viability of probiotics in the dairy food matrix as well as in the gut. Multiple efforts have been done in the past and ongoing in present for these beneficial microbial population stability until their destination in the gut. One of a useful technique known as microencapsulation makes the probiotic effective in the diversified conditions and maintain these microbe’s community to the optimum level for achieving targeted benefits. Dairy products are found to be an ideal vehicle for probiotic incorporation. It has been seen that the encapsulated microbial cells show higher viability than the free cells in different processing and storage conditions as well as against bile salts in the gut. They make the food functional when incorporated, without affecting the product sensory characteristics.
Kinetic studies on malachite green dye adsorption from aqueous solutions by A...Open Access Research Paper
Water polluted by dyestuffs compounds is a global threat to health and the environment; accordingly, we prepared a green novel sorbent chemical and Physical system from an algae, chitosan and chitosan nanoparticle and impregnated with algae with chitosan nanocomposite for the sorption of Malachite green dye from water. The algae with chitosan nanocomposite by a simple method and used as a recyclable and effective adsorbent for the removal of malachite green dye from aqueous solutions. Algae, chitosan, chitosan nanoparticle and algae with chitosan nanocomposite were characterized using different physicochemical methods. The functional groups and chemical compounds found in algae, chitosan, chitosan algae, chitosan nanoparticle, and chitosan nanoparticle with algae were identified using FTIR, SEM, and TGADTA/DTG techniques. The optimal adsorption conditions, different dosages, pH and Temperature the amount of algae with chitosan nanocomposite were determined. At optimized conditions and the batch equilibrium studies more than 99% of the dye was removed. The adsorption process data matched well kinetics showed that the reaction order for dye varied with pseudo-first order and pseudo-second order. Furthermore, the maximum adsorption capacity of the algae with chitosan nanocomposite toward malachite green dye reached as high as 15.5mg/g, respectively. Finally, multiple times reusing of algae with chitosan nanocomposite and removing dye from a real wastewater has made it a promising and attractive option for further practical applications.
Testing the efficacy of different fungicides to control different strains of potato late bight
1. CONFIDENTIAL
A. Evenhuis, G.B.M. van den Bosch & H.T.A.M. Schepers
Testing the efficacy of different fungicides to
control different strains of potato late blight
Praktijkonderzoek Plant & Omgeving (Applied Plant Research), part of Wageningen UR
Business Unit AGV PPO no.
3250281500
August 2013