1. TDM OF DIGOXIN
By,
Surya Prakash singh
ROLL: 12117D3003
pharmD IV th
year

2. IT'S A CARDIAC GLYCOSIDE
THE SOURCE'S ARE
DIGITALIS LANNATA
DIGITALIS PURPUREA
ITS STRUCTURE
Basic cardenolide with
three digitoxose untis
bound to streroidal ring
by a glycosidic linkage.
DRUG PROFILE OF DIGOXIN

3. DOSE AND INDICATION
DOSE:
Tablet : 0.125 - 0.25 mg TAB
Injection solution : 0.1mg/ML - 0.25mg/ML
INDICATION:
heart failure
&
atrial fibrillation.

4. ABSORPTION
BA: 55-90%
ONSET OF ACTION
PO : WITHIN 2 HR
IV : WITHIN 30 MIN
DURATION OF ACTION : 3-4 DAYS
PEAK PLASMA TIME : 1 HR

5. DISTRIBUTION
PROTEIN BOUND: 20-25%
VD : 4 – 7 L/KG
METABOLISM
LIVER
ELIMINATIOIN
T 1/2 : 1-3 DAYS
EXCRETION
URINE : 80%
FEACES: 10-15%

6. MECHANISM OF ACTION OF DIGOXIN

7. OTHER EFFECTS :
Neuro hormonal effects
• Decrease sympathic out flow
• Increase parasympathetic outflow
• Improve baro receptor functioning
DECREASE HEART
RATE
(NEGATIVE
CHRONOTROPIC
EFFECT)

11. REASONS FOR TDM OF
DIGOXIN ??????

13. METHOD OF ASSAY
Radioimmunoassay (RIA) involves the quantitation of drug using a
radioactive probe by measuring the radioactivity correlated to
amount of drug.
The technique was introduced in 1960 by Berson and Yalow

14. Mixture of radioactive labeled
antigen "hot"
+
antibody
Adding the sample unlabeled
antigen (drug)"cold"
The unlabelled antigen
displaces the labeled ones
(Ag-Ab)Complexes are
removed
Supernatant remains are
analyzed

15. Gamma Counter

16.  From these data, a
standard binding
curve, like the one
shown in red, can
be drawn.

18. OTHER METHODS OF ASSAYS
 Nucleic acid labeling
 Fluorescence labeling
 ELISA
 High throughput screening wells coated with
antibodies.

19. THANK YOU