The document discusses quotes and topics from past Lake Arrowhead Microbial Genomes (#LAMG16) meetings from 1998-2016. It provides quotes from various speakers over the years that range from descriptions of scientific concepts to more lighthearted comments. It also charts the relative frequency of topics discussed at the meetings over time, such as the rise of metagenomics and microbiome studies in recent years. The document serves to give a sense of the history and evolution of the #LAMG16 meetings through highlights from two decades.

1. #LAMG16 @phylogenomics
Arrowhead and Microbial Genomes:
Update on the Tree of Life
September 18, 2016
Lake Arrowhead Microbial Genomes
#LAMG16
Jonathan A. Eisen
University of California, Davis
@phylogenomics

2. TWO KEY THINGS TO REMEMBER
#LAMG16 @phylogenomics
Arrowhead and Microbial Genomes:
Update on the Tree of Life
September 18, 2016
Lake Arrowhead Microbial Genomes
#LAMG16
Jonathan A. Eisen
University of California, Davis
@phylogenomics

3. TWO KEY THINGS TO REMEMBER
#LAMG16 @phylogenomics
Arrowhead and Microbial Genomes:
Update on the Tree of Life
September 18, 2016
Lake Arrowhead Microbial Genomes
#LAMG16
Jonathan A. Eisen
University of California, Davis
@phylogenomics

4. #LAMG16 @phylogenomics
Arrowhead and Microbial Genomes:
Update on the Tree of Life
September 18, 2016
Lake Arrowhead Microbial Genomes
#LAMG16
Jonathan A. Eisen
University of California, Davis
@phylogenomics

5. #LAMG16 @phylogenomics
Arrowhead and Microbial Genomes:
Update on the Tree of Life
September 18, 2016
Lake Arrowhead Microbial Genomes
#LAMG16
Jonathan A. Eisen
University of California, Davis
@phylogenomics

6. #LAMG16 @phylogenomics
The Tree of Life
http://phylogenomics.blogspot.com

7. #LAMG16 @phylogenomics
Arrowhead and Microbial Genomes:
Update on the Tree of Life
September 18, 2016
Lake Arrowhead Microbial Genomes
#LAMG16
Jonathan A. Eisen
University of California, Davis
@phylogenomics

8. Arrowhead and Microbial Genomes:
Update on the Tree of Life
September 18, 2016
Lake Arrowhead Microbial Genomes
#LAMG16
Jonathan A. Eisen
University of California, Davis
@phylogenomics
THREE KEY THINGS TO REMEMBER
#LAMG16 @phylogenomics

9. Origin of the Meeting
• Who knows how the meeting started?
#LAMG16 @phylogenomics

10. Origin of the Meeting
• Who knows how the meeting started?
• How could you find out?
#LAMG16 @phylogenomics

11. Origin of the Meeting
• Who knows how the meeting started?
• How could you find out?
• The Google …
#LAMG16 @phylogenomics

12. Using the Google
#LAMG16 @phylogenomics

13. Using the Google
#LAMG16 @phylogenomics

14. Using the Google
#LAMG16 @phylogenomics

15. Possible Source of Confusion
#LAMG16 @phylogenomics
Jeffrey van Leeuwenhoek Miller

16. History of the Meeting
• See http://
www.lamg.info/
history
#LAMG16 @phylogenomics
Jeffrey van Leeuwenhoek Miller

17. History of the Meeting
• And see http://microbe.net/lake-arrowhead-
microbial-genomes-history/ for 1998-2016 notes
• http://tinyurl.com/LAMG16
#LAMG16 @phylogenomics

18. Sampling from 1998-2014
#LAMG16 @phylogenomics

19. Sampling from 1998
#LAMG16 @phylogenomics

20. Sampling from 1998
#LAMG16 @phylogenomics

21. Sampling from 1998
#LAMG16 @phylogenomics

22. The Real Story of 1998?
#LAMG16 @phylogenomics

23. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

24. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

25. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

26. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

27. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

28. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

29. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

30. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

31. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

32. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

33. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

34. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

35. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

36. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

37. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

38. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

39. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

40. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

41. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

42. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

43. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

44. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

45. 1998 - Doodles Reveal All
#LAMG16 @phylogenomics

46. Real History of Arrowhead
QUOTES, QUOTES, QUOTES
#LAMG16 @phylogenomics

47. 2014 …
#LAMG16 @phylogenomics

48. 2014 …
• I am spending bioinformatic time up the
wazoo
#LAMG16 @phylogenomics

49. 2014 …
• I am spending bioinformatic time up the
wazoo
• It may be sh*t to you but it's my bread and
butter
#LAMG16 @phylogenomics

50. 2002
#LAMG16 @phylogenomics

51. 2002
• Leave it to Jeff Miller to take a shot at the
Gordon Conferences in his intro
#LAMG16 @phylogenomics

52. 2002
• Leave it to Jeff Miller to take a shot at the
Gordon Conferences in his intro
• It looks like someone with a bad cold
sneezed against the wall
#LAMG16 @phylogenomics

53. 2002
• Leave it to Jeff Miller to take a shot at the
Gordon Conferences in his intro
• It looks like someone with a bad cold
sneezed against the wall
• Wild mistakes - brilliant guesses
#LAMG16 @phylogenomics

54. 2002
• Leave it to Jeff Miller to take a shot at the
Gordon Conferences in his intro
• It looks like someone with a bad cold
sneezed against the wall
• Wild mistakes - brilliant guesses
• He lied about the size of anthrax
#LAMG16 @phylogenomics

55. 2002
• Leave it to Jeff Miller to take a shot at the
Gordon Conferences in his intro
• It looks like someone with a bad cold
sneezed against the wall
• Wild mistakes - brilliant guesses
• He lied about the size of anthrax
• If it grows you win, if it doesn’t you have
to give me your soul for all eternity
#LAMG16 @phylogenomics

56. 2002
• Leave it to Jeff Miller to take a shot at the
Gordon Conferences in his intro
• It looks like someone with a bad cold
sneezed against the wall
• Wild mistakes - brilliant guesses
• He lied about the size of anthrax
• If it grows you win, if it doesn’t you have
to give me your soul for all eternity
• That’s because she has the arrogance of
the aristocrats
#LAMG16 @phylogenomics

57. 2004
#LAMG16 @phylogenomics

58. 2004
• He wanted to keep his finger in the
Chlamydia stuff
#LAMG16 @phylogenomics

59. 2004
• He wanted to keep his finger in the
Chlamydia stuff
• Engineering other systems that don’t
suck
#LAMG16 @phylogenomics

60. 2004
• He wanted to keep his finger in the
Chlamydia stuff
• Engineering other systems that don’t
suck
• It’s like a tootsie pop
#LAMG16 @phylogenomics

61. 2004
• He wanted to keep his finger in the
Chlamydia stuff
• Engineering other systems that don’t
suck
• It’s like a tootsie pop
• Space time continuum of genes and
genomes
#LAMG16 @phylogenomics

62. 2004
• He wanted to keep his finger in the
Chlamydia stuff
• Engineering other systems that don’t
suck
• It’s like a tootsie pop
• Space time continuum of genes and
genomes
• The wormhole that allows us to tunnel
into today’s world
#LAMG16 @phylogenomics

63. Other years
• Space-time continuum of genes and
genomes
#LAMG16 @phylogenomics

64. Quotes
• Space-time continuum of genes and
genomes
• Microbes not only have a lot of sex, they
have a lot of weird sex
#LAMG16 @phylogenomics

65. Quotes
• Space-time continuum of genes and
genomes
• Microbes not only have a lot of sex, they
have a lot of weird sex
• Gene sequences are the wormhole that
allows one to tunnel into the past
#LAMG16 @phylogenomics

66. Quotes
• Space-time continuum of genes and
genomes
• Microbes not only have a lot of sex, they
have a lot of weird sex
• Gene sequences are the wormhole that
allows one to tunnel into the past
• This is how you do metagenomics on 50
dollars, and that’s Canadian dollars
#LAMG16 @phylogenomics

67. Quotes
• Space-time continuum of genes and
genomes
• Microbes not only have a lot of sex, they
have a lot of weird sex
• Gene sequences are the wormhole that
allows one to tunnel into the past
• This is how you do metagenomics on 50
dollars, and that’s Canadian dollars
• The human guts are a real milieu of stuff
#LAMG16 @phylogenomics

68. Quotes
• Space-time continuum of genes and
genomes
• Microbes not only have a lot of sex, they
have a lot of weird sex
• Gene sequences are the wormhole that
allows one to tunnel into the past
• This is how you do metagenomics on 50
dollars, and that’s Canadian dollars
• The human guts are a real milieu of stuff
• Antibiotics do not kill things, they corrupt
them #LAMG16 @phylogenomics

69. Quotes
• There comes a point in life when you have
to bring chemists into the picture
#LAMG16 @phylogenomics

70. Quotes
• There comes a point in life when you have
to bring chemists into the picture
• The rectal swabs are here in tan color
#LAMG16 @phylogenomics

71. Quotes
• There comes a point in life when you have
to bring chemists into the picture
• The rectal swabs are here in tan color
• If I have time I will tell you about a dream
#LAMG16 @phylogenomics

72. Quotes
• There comes a point in life when you have
to bring chemists into the picture
• The rectal swabs are here in tan color
• If I have time I will tell you about a dream
• Another thing you need to know" pause
"Actually you don't NEED to know any of
this
#LAMG16 @phylogenomics

73. Quotes
• There comes a point in life when you have
to bring chemists into the picture
• The rectal swabs are here in tan color
• If I have time I will tell you about a dream
• Another thing you need to know" pause
"Actually you don't NEED to know any of
this
• I have been influenced by Fisher Price
throughout my life
#LAMG16 @phylogenomics

74. Quotes
• There comes a point in life when you have
to bring chemists into the picture
• The rectal swabs are here in tan color
• If I have time I will tell you about a dream
• Another thing you need to know" pause
"Actually you don't NEED to know any of
this
• I have been influenced by Fisher Price
throughout my life
• This is going to be ironic coming from
someone who studies circumcision#LAMG16 @phylogenomics

75. Quotes
• And we will bring out the unused cheese
from yesterday
#LAMG16 @phylogenomics

76. Quotes
• And we will bring out the unused cheese
from yesterday
• A paper came out next year
#LAMG16 @phylogenomics

77. Quotes
• And we will bring out the unused cheese
from yesterday
• A paper came out next year
• It takes 1000 nanobiologists to make one
microbiologist
#LAMG16 @phylogenomics

78. Quotes
• And we will bring out the unused cheese
from yesterday
• A paper came out next year
• It takes 1000 nanobiologists to make one
microbiologist
• In an engineering sense, the vagina is a
simple plug flow reactor
#LAMG16 @phylogenomics

79. Quotes
• And we will bring out the unused cheese
from yesterday
• A paper came out next year
• It takes 1000 nanobiologists to make one
microbiologist
• In an engineering sense, the vagina is a
simple plug flow reactor
• The human mind can conceive of things
with no basis in physical reality
#LAMG16 @phylogenomics

80. Quotes
• And we will bring out the unused cheese
from yesterday
• A paper came out next year
• It takes 1000 nanobiologists to make one
microbiologist
• In an engineering sense, the vagina is a
simple plug flow reactor
• The human mind can conceive of things
with no basis in physical reality
• Thoughts can go faster than the speed of
light #LAMG16 @phylogenomics

81. Quotes
• We are the environment. We live the
phenotype.
#LAMG16 @phylogenomics

82. Quotes
• We are the environment. We live the
phenotype.
• We have been using this word for many
years without actually realizing it was
correct
#LAMG16 @phylogenomics

83. Quotes
• We are the environment. We live the
phenotype.
• We have been using this word for many
years without actually realizing it was
correct
• A little bit about time, but I am going to
spend a lot less time on time than on
space
#LAMG16 @phylogenomics

84. Quotes
• We are the environment. We live the
phenotype.
• We have been using this word for many
years without actually realizing it was
correct
• A little bit about time, but I am going to
spend a lot less time on time than on
space
• I am going to wrap up as I hear the
crickets chirping
#LAMG16 @phylogenomics

85. 2004
#LAMG16 @phylogenomics

86. Sampling from 2004
#LAMG16 @phylogenomics

87. Sampling from 2004
#LAMG16 @phylogenomics

88. Sampling from 2004
#LAMG16 @phylogenomics

89. Sampling from 2004
#LAMG16 @phylogenomics

90. Sampling from 2004
#LAMG16 @phylogenomics

91. Sampling from 2004
#LAMG16 @phylogenomics

92. Sampling from 2004
#LAMG16 @phylogenomics

93. Sampling from 2004
#LAMG16 @phylogenomics

94. Sampling from 2004
#LAMG16 @phylogenomics

95. Trends in Topics
#LAMG16 @phylogenomics

96. Topics Over Time
0
25
50
75
100
1998 2000 2002 2004 2006 2008 2010 2012 2014 2016
Genomes Communities
Metagenomes Microbiomes

97. Topics Over Time
0
25
50
75
100
1998 2000 2002 2004 2006 2008 2010 2012 2014 2016
Genomes Communities
Metagenomes Microbiomes

98. Topics Over Time
0
25
50
75
100
1998 2000 2002 2004 2006 2008 2010 2012 2014 2016
Genomes Communities
Metagenomes Microbiomes

99. Topics Over Time
0
25
50
75
100
1998 2000 2002 2004 2006 2008 2010 2012 2014 2016
Genomes Communities
Metagenomes Microbiomes

100. Topics Over Time
0
25
50
75
100
1998 2000 2002 2004 2006 2008 2010 2012 2014 2016
Genomes Communities
Metagenomes Microbiomes

101. Topics Over Time
0
25
50
75
100
1998 2000 2002 2004 2006 2008 2010 2012 2014 2016
Genomes Communities
Metagenomes Microbiomes

102. Topics Over Time
0
25
50
75
100
1998 2000 2002 2004 2006 2008 2010 2012 2014 2016
Genomes Communities
Metagenomes Microbiomes

103. Topics Over Time
0
25
50
75
100
1998 2000 2002 2004 2006 2008 2010 2012 2014 2016
Genomes Communities
Metagenomes Microbiomes

104. Topics Over Time
0
25
50
75
100
1998 2000 2002 2004 2006 2008 2010 2012 2014 2016
Genomes Communities
Metagenomes Microbiomes

105. Topics Over Time
0
25
50
75
100
1998 2000 2002 2004 2006 2008 2010 2012 2014 2016
Genomes Communities
Metagenomes Microbiomes

106. Possible Talks for 2016?
#LAMG16 @phylogenomics

107. Possible Talks for 2018?
#LAMG16 @phylogenomics

108. Microbiome Will GIVE The Talks
#LAMG16 @phylogenomics

109. #LAMG16 Speakers
#LAMG16 @phylogenomics