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SyMAP Synteny Mapping and Analysis Program Austin Shoemaker
SyMAP Team ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Background ,[object Object],[object Object],[object Object],[object Object]
Comparative Genomics ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Genome Rearrangements Rearrangement  Scenario  Result Inversion Duplication Insertion Deletion A BCD E  A DCB E A B C  AC AB  A C B A B  CD  A B   B CD A B C  A B C B , A BB C AB   AB   AB
Whole-Genome Duplication ,[object Object],Last Common Ancestor rice maize diverged 50-70 mya 70 mya duplication 11 mya duplication
Synteny ,[object Object],[object Object],[object Object],[object Object],Genome A c d e f g c d e f g Genome B
Physical Map ,[object Object],[object Object]
FPC Map ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Making a BAC Clone Library ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Clones ,[object Object],[object Object]
Clone Fingerprinting ,[object Object],[object Object],[object Object]
Clone Fingerprinting ,[object Object],[object Object],[object Object],[object Object]
FPC ,[object Object],[object Object],[object Object],[object Object],[object Object]
Markers ,[object Object],[object Object],[object Object],[object Object]
BESs ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Anchors ,[object Object],[object Object],[object Object],[object Object],G G C C G T G G T G C T C T T T G C A A T G G G G G C T G T G G T G C T C T T C G C A A T G G G
Component Summary
Finding Chains
Key Synteny Finding Algorithms ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Other Synteny Finding Algorithms ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
FPC to Genome Synteny ,[object Object],[object Object],[object Object],[object Object],[object Object]
FPC Synteny Properties 1  x 2  o 3  x  4  x  5  o 6  8  #  x  9  x a  x b  x c  x 7  x 1  2  3  4  5  6  7  8  9  a  b  c Genome A (FPC map) Genome B (sequenced genome)
Noise
SyMAP Algorithm ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
SyMAP Algorithm ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
SyMAP Algorithm ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Sytry ,[object Object],[object Object],[object Object],[object Object],[object Object]
Automated Parameter Setting ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Sub-Chains ,[object Object]
Sub-Chain Finder ,[object Object],[object Object],[object Object]
Sub-Chains ,[object Object],[object Object],[object Object],[object Object]
BES Clone End Assignments ,[object Object],[object Object],[object Object],[object Object]
BES Clone End Assignments ,[object Object],2  x 3  o 4  5  6  o 7  x 8  x 1  2  3  4  5  6  7  8 A B 1  x B A 2 3 4 5 6 7 8 2 3 4 5 6 7 8 1 1
BES Clone End Assignments ,[object Object],7  x 6  o 5 4  3  o 2  x 1  x 1  2  3  4  5  6  7  8 A 8  x B B A 1 2 3 4 5 6 7 1 2 3 4 5 6 7 8 8
SyMAP Views ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
All Blocks ↔ Sequenced Chromosomes
Blocks ↔ Sequenced Chromosome
Genome ↔ Genome Dot Plot
Chromosome ↔ Chromosome Dot Plot
Block ↔ Sequenced Chromosome
Subset Flipped
Contig ↔ Sequenced Chromosome
Filters and Controls
FPC ↔ Sequenced Chromosome ↔ FPC
FPC ↔ FPC
Close-up of Gene
SyMAP Implementation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Results ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Acknowledgements ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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SyMAP Master's Thesis Presentation

Editor's Notes

  1. Hello. I am Austin Shoemaker and I’m going to be presenting SyMAP, a synteny mapping and analysis program.